10 Phenolic Acids Analyzed with LCMS – AppNote

Compounds Shown were Extracted from Commercial Rice. Commercial Rice Extract 3 was spiked with ten standards and analyzed. The Cogent Phenyl Hydride Column was an excellent choice to use for analysis of Phenolic Compounds with Reversed Phase HPLC. The Retention and Separation of all nine available standards was possible using the Method below.   Peaks: […]

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4’-Aminoacetophenone Analysis with HPLC – AppNote

4’-Aminoacetophenone Retained in Acidic Conditions This Method investigated 4’-Acetaminophenone Retention and Analysis. Adequate Retention and Sensitivity was the result and could be useful in Analysis of the Metabolites of this class of Anti-tumor Agents in body fluids (Plasma or Urine) during or after Chemotherapy. Peak: 4’-Aminoacetophenone Method Conditions Column: Cogent Bidentate C18™, 4μm, 100Å Catalog […]

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5 Phenolic Compounds Separated by LCMS – AppNote

Commercial Rice Extract A commercial rice extract was analyzed and peaks were assigned based on retention times and m/z values for the compounds of interest using the Cogent Phenyl Hydride Column . After method validation the developed procedure can be used to evaluate the quality of rice and to develop the best extraction procedure. Peaks: […]

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Acetaminophen & Impurity Analyzed with HPLC – AppNote

APAP Drug Substance Analysis with HPLC This Method has been developed for determination of Acetaminophen as a Drug Substance. The Separation was achieved on a C18 Column using Gradient Elution. The Method shown here has excellent Reproducibility of Acetaminophen for 5 consecutive Chromatograms shown had 0.03% RSD. This could be used for Quality Control of […]

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Acetaminophen Impurities Method with HPLC – AppNote

Acetaminophen – Alternate USP Method Acetaminophen and two of its major impurities were analyzed using a simple Mobile Phase. The Peak Shapes are very good and the Repeatability was good (%RSD = 0.01). Peaks: 1. 4-Aminophenol 1.072 minutes 2. Acetaminophen 4.668 minutes 3. 4-nitrophenol 7.588 minutes Method Conditions: Column: Cogent Bidentate C18™, 4µm, 100Å Catalog […]

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Advantage of Using Cogent TYPE-C Columns for Drugs of Abuse – Extended AppNote

Heroin, MDMA, Morphine, Hydromorphone and 6-MAM Analyzed with LCMS Analysis of Drugs of Abuse encompasses a wide variety of compounds, including controlled substances, prescription drugs, and even over-the-counter formulations. These drugs can be problematic to analyze due to several problems. First, many have similar structures (e.g. morphine and hydromorphone), making adequate selectivity difficult to obtain […]

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Aegeline in Protein Sport Mix – AppNote

LC-MS analysis of harmful substance in food supplement The data demonstrates the use of the Cogent Bidentate C18 2.o™ Column for analysis of dietary supplement samples. Isolation of the Aegeline peak is readily obtained with an extracted ion chromatogram (EIC) in the positive ion mode. Chromatographically, the peak is well retained and highly symmetrical. The […]

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Amlodipine, Valsartan, and Hydrochlorothiazide Tablet USP Assay – AppNote

This AppNote demonstrates the USP Method for Amlodipine, Valsartan, and Hydrochlorothiazide Tablets working with the Cogent RP C18. As shown in the Chromatograms, the Peak efficiency is superb and USP Peak tailing guidelines are easily met with the Column. This demonstrates a great alternative Column for easy plug and play with this USP Method. Method […]

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Amoxicillin USP Assay with HPLC – AppNote

Alternate L1 Column used for the USP Assay Method for Amoxicillin This USP Amoxicillin Assay is performed and a five injection overlay shows Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values less than 0.3%. This demonstrates a good alternate Column for […]

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Amoxicillin, USP Method with HPLC – AppNote

Robust Analysis of Amoxicillin, Antibiotic with HPLC The USP Assay Monograph Method for Amoxicillin often yields poor Peak shapes and low Method reproducibility with L1 Columns based on ordinary Silica. In contrast, the Figures shown here illustrate the high degree of reproducibility obtained from use of the Cogent Bidentate C18 L1 Column, following the USP […]

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Analysis of Alpha and Beta-Acids in Hops with HPLC – AppNote

Robust, Precise Method for Beer Analysis with HPLC The effectiveness of this Method is evident from the fact that data obtained were in a good agreement with the literature values. This Method also has the added bonus of good Precision (%RSD =0.1 and below). Calibration Curves for Alpha and Beta Acids in Beer showed good […]

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Analysis of Harmful Substances in Dietary Supplements – Extended AppNote

Dietary Supplements have had, in notable cases, ingredients added to them that have been implicated in serious health issues and even death. Two such pernicious compounds are Aegeline and 1,3-Dimethylamylamine. These two can be readily separated from the dietary supplement matrix using the Cogent Bidentate C18 2.o™   and Cogent Diamond Hydride™ Columns by LCMS […]

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Analysis of Isobaric Opioids by LCMS – AppNote

Separation of Isobaric Drugs Morphine and Hydromorphone are Isobaric Drugs (the same m/z value) that can be separated using the Cogent Bidentate C18 2.o™ Column. Multiple samples (n=5) were prepared and analyzed. The results showed great reproducibility (RSDs < 3%). The detection limits were 50 to 500 times lower than that required by immunoassays. Peaks: […]

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Analysis of MDMA in Plasma Samples with LCMS – AppNote

Methylenedioxymethamphetamine Analyzed with MS Under the described conditions, MDMA was retained and eluted as a Symmetrical Peak. The Sensitivity of the Method is very good and  comparable to that reported with GCMS Detection [1]. Matrix effects were of minor extent and reproducible and hence should not compromise Quantification. The Method can be used for Forensic […]

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Aspirin & Salicylic Acid Analyzed with HPLC- AppNote

Excellent Peak Shape For a Very Difficult Compound This Method is easy to prepare, use, reproduce and a good Separation of Aspirin from its major hydrolysis product, Salicylic Acid is achieved. Peaks: 1. System Peak 2. Aspirin (Acetylsalicylic Acid) 3. Salicylic Acid Method Conditions Column: Cogent Bidentate C18™, 4μm, 100Å Catalog No.: 40018-75P Dimensions: 4.6 x […]

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Atorvastatin Drug Product Analyzed with HPLC

API Well Separated from Degradation Product Using this Simple Linear Gradient, Atorvastatin is well separated from its main degradation Product. The degradant is formed under acidic conditions and therefore its Separation is important for Stability Indicating Methods for pharmaceutical formulations of Atorvastatin. For Methods requiring non-degrading conditions, it is important to choose diluent and Mobile […]

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Atorvastatin HPLC Method Transfer to UHPLC – AppNote

Transfer to “near-UHPLC” 2.2um Column, for the API Atorvastatin This Application Note demonstrates how data obtained on a 4μm Cogent Bidentate C18 Column (Figure B) can be adapted for a 2.2μm Column (Figure A). The retention times of both analytes are very comparable in each figure. This Method produces excellent Separation of the API and […]

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Atropine Analyzed with a Gradient Method – AppNote

No Ion Pair Reagent Necessary Chromatographic separation and quantification methods of Atropa Alkaloids are often described in the literature and the method of choice is usually Ion-Pair Chromatography (IPC), which requires long equilibration times and it is not very robust. This method shows a symmetrical peak for Atropine using a simple Gradient Method that does […]

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Barbiturates Analyzed with LCMS – AppNote

Standard Solutions in Human Urine Spiked with Barbiturates After minimal sample preparation (dilute-and-shoot approach), spiked urine samples were analyzed using a C18 Column. The analysis was based on a Separation of Standards [1]. The obtained Peaks were Symmetrical (As<1.05) and Efficient (> 106 pl/m). No shift in retention Times was observed after the samples were diluted […]

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Benzonatate Excipients & Degradants Analyzed with HPLC – AppNote

A Simple, Linear Gradient for a Challenging Drug Product This Gradient Method will produce a good separation of Benzonatate from excipients and degradants in a USP Capsule Formulation. Benzonatate contains a secondary amine group, which can cause peak tailing. Peak:  Benzonatate Method Conditions Column: Cogent Bidentate C18™, 4μm, 100Å Catalog No.: 40018-75P Dimensions: 4.6 x […]

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Benzophenone Analyzed with HPLC – AppNote

Isocratic Method Retains Benzophenone with Efficiency and Precision Benzophenone, a hydrophobic non ionic compound, was selected to illustrate the excellent Reversed Phase HPLC capabilities of this Cogent TYPE-C Silica Hydride Stationary Phase. The Reproducibility, Efficiency and Peak Symmetry of this Method is excellent, as presented in each figure below, which represents ten consecutive injections with […]

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Bi-Est® Method Transfer – AppNote

4μm to Near-UHPLC – Separation of Hormones: Estriol, Estradiol, and Progesterone Separation of three components of a hormone replacement formulation is demonstrated in this Application Note using the Cogent Bidentate C18 2.o™ Column. The two figures demonstrate how comparable retention can be obtained for both the near-UHPLC column as well as the standard 4μm Column, […]

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Bisacodyl Tablet Analyzed with HPLC – AppNote

Separation of API from Matrix Peaks from an Over The Counter Tablet This Method for Analysis of a Bisacodyl Tablet formulation Separates the API from several small matrix Peaks. These Peaks must be Resolved from the main Peak in order to ensure accurate Quantitation. The data below illustrates the separation potential of the this Method […]

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Bisphenol A, BPA Analyzed with HPLC – AppNote

Toxic Substance Found in Consumer Products Analyzed with HPLC Bisphenol A (BPA) is a challenging compound for analysis with HPLC. Monitoring of this environmental and health toxicant is a necessary process for surveillance as well as risk assessment. As shown in the 5 Chromatograms overlaid below, this Method produces Peak Shapes that are Symmetrical and […]

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Bisphenol A, S, & F Analyzed with HPLC – AppNote

Separation of Epoxy Resins Found in Consumer Products & Food Many consumer products are using Bisphenol A (BPA) alternatives in recent years due to health concerns regarding toxicity of these compounds.  An analytical HPLC method is shown in this AppNote to distinguish amongst these various Bisphenol compounds potentially in chemicals and foods. Excellent resolution was […]

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Caffeine Content in Coffee Analyzed with HPLC – AppNote

Espresso, Regular, and De-Caffeinated Coffee Caffeine Content Caffeine is separated from other matrix components in three common Coffee beverages in this AppNote. A shot of Espresso (Figure A) had the highest content, followed by regular drip Coffee (Figure B) as expected. In De-Caffeinated Coffee, Caffeine is extracted from the coffee beans but even after numerous […]

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Cancer Prodrug Analyzed with HPLC – AppNote

Separation Achieved with Shape Selectivity and Hydrophobic Interaction This Method provides an example of Reversed Phase Separation containing a number of related compounds. The properties of the solutes are listed in the Table below. When comparing the properties of Compounds 2 and 3, it is interesting to note that 2 is more hydrophobic than 3 […]

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Cannabis Metabolite Analyzed with LCMS – AppNote

THC Metabolite Found in Urine Matrix Analyzed The extraction procedure using an SPE technique allows for successful detection of this Cannabis metabolite with this Method. It is worth noting that the Retention of this Compound was achieved using Methanol rather than Acetonitrile as the Organic component in the Mobile Phase. Peak: (l)-9-Carboxy-11-Nor-Delta-9-Tetrahydrocannabinol, m/z 345.2060 [M+H]+ […]

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Capsaicin in Topical Solution Analyzed with HPLC – AppNote

Separation of Capsaicinoids in an OTC Formulation A  formulation of Capsaicins is used as a topically applied solution for Arthritis pain relief. Analyzing Capsaicin and a similar compound, Dihydrocapsaicin is required. The Method illustrates the ability to resolve structurally similar Capsaicinoids from a pharmaceutical formulation. Peak Shapes and Efficiencies are excellent for both Peaks. Furthermore, […]

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Carisoprodol & Meprobamate Analysis by LCMS – AppNote

Analysis of Closely Related Compounds It has been reported that for the quantitation of Meprobamate and Carisoprodol, (in biological samples plasma, urine, hair etc) by LCMS, excessive analysis time and complex sample treatment (derivatization, solid or liquid phase extraction) may be required. The Method in this AppNote shows a rapid way to quantitate both drugs […]

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Carotenoids Analyzed with HPLC – AppNote

Compounds of the Carotenoid Family Retained These compounds are very lipophilic and often have subtle differences in structure. Resolution of these compounds can be difficult with a standard Reversed Phase C8 or C18 Column because of these Similarities. The Cogent C30 Column on the other hand can further differentiate by analyte Shape in addition to […]

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Cefpodoxime Proxetil Analyzed with HPLC – AppNote

USP Assay Method an Antibiotic Prodrug The USP Assay Method for Cefpodoxime Proxetil specifies a Resolution of not less than 2.5 between the two epimers of the Prodrug. Using this Method, the average Resolution was calculated to be 2.8. In addition, the R-epimer tailing factor must be not more than 1.5 where this Method meets […]

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Cefprozil E & Z Isomers Analyzed with HPLC – AppNote

LCMS Compatible Method The USP Assay Method for Cefprozil uses a phosphate-based Mobile Phase, which is not compatible with LCMS. In this Method, only Formic Acid is needed in the Mobile Phase. The Method meets the isomer resolution criterion of not less than 2.5 with an average calculated value of 4.4. The tailing factor was […]

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Celecoxib Analyzed with HPLC – AppNote

Impurities Method with Improved Peak Shape Celecoxib is Separated from an Impurity in this simple Gradient Method. The Peak shape obtained for this API shows good Peak Symmetry. In addition, the Method shows good repeatability from run to run. The API Retention time %RSD from five runs was 0.025%. Figure A shows the excellent Precision […]

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Chlorpheniramine Maleate Organic Impurities Analyzed with HPLC – AppNote

Separation demonstrated in real commercial formulation extracts Use of the Cogent Bidentate C8™ Column allows for high resolution baseline separation of six specified impurities of Chlorpheniramine Maleate (Fig. A). Fig. B shows how the method can be applied to a real-world formulation, spiked with the N-oxide impurity to demonstrate resolution from the API peak. Currently, […]

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Clindamycin Antibiotic Tablet Analyzed with HPLC – AppNote

Separation of API from Excipients  The Stability and Robustness of this Method makes it an excellent choice for QC of this semi-synthetic antibiotic which is structurally related to Lincomycin. The Chromatogram shown below of Clindamycin is from a complex Pharmaceutical Formulation but note the excellent Peak Shape for it and the Separation from the Excipients. […]

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Codeine and Hydrocodone in Blood Analyzed with LCMS – AppNote

LCMS Separation of isobaric Drugs Found in Urine Codeine and Hydrocodone which are Isobaric Drugs (i.e. the same m/z value) were separated using this AppNote. Multiple Samples (n=5) were prepared and analyzed. The results showed excellent Reproducibility (RSDs < 2.5%). The Method in this AppNote, after validation, can be used in Forensic Toxicological Laboratories to […]

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Comparison of 4um vs. 2.2um Columns – Extended AppNote

Easy Method Transfer from 4µm to 2.2µm HPLC Columns. The Bidentate C18™ is an excellent choice for Reversed Phase Applications. In addition to the standard 4µm material, a near UHPLC 2.o™ (2.2um) Phase is also available. These 2.2µm Columns provide inherently superior Efficiency than a larger particle size. Because the same C18 ligand is used […]

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Coricidin HBP Day Tablet Analyzed with HPLC- AppNote

Formulation of Guaifenesin and Dextromethorphan HBr This Method shows the separation of two active ingredients in a formulation of Guaifenesin and Dextromethorphan. Dextromethorphan often exhibits tailing in many HPLC methods, but here an excellent Peak shape is observed. This Method is suitable for routine assay of these compounds in combination formulations. A change in wavelength […]

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Coricidin HBP Night® Tablet – AppNote

Acetaminophen, Dextromethorphan, and Chlorpheniramine This Method demonstrates the potential of the Cogent Phenyl Hydride 2.o™ Column for analysis of amine-containing analytes in a real formulation. Chlorpheniramine and Dextromethorphan can often be problematic in terms of peak tailing due to the tertiary amines, but this column and method produces excellent peak shapes for both compounds in this […]

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Cough Syrup Ingredient Analysis with HPLC – AppNote

Separation of Antitussives, Analgesics, Decongestants, and Preservatives Cold and cough formulations may contain a number of components such as antitussives (Dextromethorphan), decongestants (Pseudoephedrine, Guaifenesin), analgesics (Acetaminophen), and preservatives (Methyl Paraben, Propyl Paraben, Benzoic Acid). The method illustrates good separation between a variety of these compounds, also that symmetric Peak shapes can be obtained in each […]

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Cyanidin-3-O-Glucoside Analyzed in Fruit – AppNote

Analysis in Fruit and Vegetable Extracts In this analysis of proprietary fruit & vegetable extracts, the presence of Cyanidin-3-O-Glucoside was confirmed in three out of four extracts. The peaks were Symmetrical and Retained beyond the dead volume. The Method after validation can be used to quality control of commercial fruit extracts as well in studies […]

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Cyclobenzaprine Hydrochloride USP Assay – AppNote

This method demonstrates the USP Cyclobenzaprine Hydrochloride Extended-Release Capsules Assay. As shown in the Chromatograms, the peak efficiency is superb and USP peak tailing guidelines are easily met with the Cogent RP C18 5μm column. This demonstrates a great alternative column for easy plug and play and this UPS Assay Method. Method Conditions: Column: Cogent […]

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Cytarabine Assay with HPLC- AppNote

A Reliable Assay for Detection of a Chemotherapeutic   Cytarabine belongs to the category of chemotherapy called antimetabolites. Antimetabolites are very similar to normal substances within the cell. When these substances are incorporated into the cellular metabolism, they are unable to divide. Antimetabolites are cell-cycle specific and target cells at very specific phases in the […]

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Cytochrome C Analyzed with HPLC – AppNote

From Horse and Bovine Heart Using the simple Reversed Phase HPLC Gradient Method shown in this note, it was possible to separate Horse and Bovine heart Cytochrome C. The Peaks were well separated and symmetrical. A linear detector response was observed over 2 orders of magnitude. Peaks: 1. Cytochrome C from Horse heart 2. Cytochrome […]

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Dehydroepiandrosterone (DHEA) Capsule Separation with HPLC – AppNote

Excellent separation from matrix excipients Pharmaceutical formulations may contain a number of excipients that can potentially interfere with a chromatographic analysis. However, using the Cogent Bidentate C8 Column, the DHEA peak is well-resolved from the others, the latter of which all elute as a large peak in the solvent front portion of the run. The […]

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Deoxycholic Acid Assay Analyzed with HPLC – AppNote

A Reliable Method for a Bile Acid This Deoxycholic Acid Assay produces Run to Run consistency and Precision with Retention Time RSD values of 0.1%. A sensitive HPLC-UV Method that may be used for quantification in raw material and liquid pharmaceutical formulation is presented. Figure 1. Full Chromatogram Figure 2. Magnified Chromatogram to show peak […]

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