Acetaminophen & Impurity Analyzed with HPLC – AppNote

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May 14, 2012
Bill Ciccone

AppNotes Cogent Bidentate C18 / AppNotes Library / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

APAP Drug Substance Analysis with HPLC

This Method has been developed for determination of Acetaminophen as a Drug Substance. The Separation was achieved on a C18 Column using Gradient Elution. The Method shown here has excellent Reproducibility of Acetaminophen for 5 consecutive Chromatograms shown had 0.03% RSD. This could be used for Quality Control of Acetaminophen.

Peaks:
1. Impurity
2. Acetaminophen

Method Conditions
Column: Cogent Bidentate C18™, 4μm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase:
—A: DI Water / 0.1% Acetic Acid / 0.005% Trifluoroacetic Acid (TFA)
—B: 100% Acetonitrile / 0.1% Acetic Acid / 0.005% Trifluoroacetic Acid (TFA)
Gradient:

Time (minutes)	%B
0	0
1	0
4	30
6	30
6.01	0
10	0

Injection vol.: 2μL
Flow rate: 1mL / minute
Detection: UV @ 254nm
Sample Preparation: 1mg of the Compound was dissolved in 1mL of 50:50 Solvent A / Solvent B solution. Sample for Injection was diluted 1:15 with 100% Solvent A.

Notes: Acetaminophen (n-acetyl-p-aminophenol, APAP) is an non-steroidal anti-inflammatory drug (NSAID) which is widely used for the management of pain and fever. Safety concerns require analyzing the composition of the pharmaceutical formulations.

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