Are the Cogent Silica-C columns stable?

Yes, we have done extensive testing in our labs and in real world use around the world and have proven that the Cogent Silica-C™ columns are stable even in the presence of water. However, these columns are designed and specified for Normal Phase and Aqueous Normal Phase HPLC and not for Reversed Phase. But the only […]

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Can I expect a more predictable retention time with Silica-C columns?

Yes, if you develop a method that is optimized with the Cogent Silica-C™ columns compared with an ordinary silica column, the retention times are generally much more reproducible. The reason is most likely that when using ordinary silica columns the uptake of water changes from run to run and the retention often changes. Since the […]

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Can I use triethylamine (TEA) with a Silica-C column?

The triethylamine (TEA) additive is compatible with Silica-C™ columns in the sample diluent or mobile phase as long as the concentration is not excessive and the mobile phase pH is kept within the specifications of the column (pH must not exceed 7.0). Specifications for Silica-C HPLC columns More information on Cogent Silica-C HPLC columns

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How do I condition new Cogent TYPE-C™ Columns for use?

For all new Cogent TYPE-C™ HPLC columns with the exception of new Diamond Hydride™ columns, all one needs to do is run 7-10 column bed volumes of your mobile phase through the column at your normal flow rate. Then it is best to inject a known standard and repeat the injections until duplicate chromatograms are […]

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Why are ANP HPLC Methods Better to use than HILIC? – FAQ

Why ANP is better than HILIC for Polar Compound Methods Compared to ANP, HILIC will require more time to Equilibrate between gradient runs, takes more salt to retain some compounds, often lacks Retention Time (RT) Precision, cannot retain polar compounds with high amounts of water in the mobile phase and Lifetime of the columns can […]

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