Bradykinin Analyzed with LCMS – AppNote

The Peptide Bradykinin Analyzed in Saliva Samples A simple, sensitive, and specific Gradient Method was developed for the Quantification of Bradykinin (BDK) in Saliva. In addition to the intact Peptide, the Gradient applied in the developed Method was designed to analyze its impurities and metabolites. Robustness of this Method, against small modifications in pH, flow […]

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Cytochrome C Analyzed with HPLC – AppNote

From Horse and Bovine Heart Using the simple Reversed Phase HPLC Gradient Method shown in this note, it was possible to separate Horse and Bovine heart Cytochrome C. The Peaks were well separated and symmetrical. A linear detector response was observed over 2 orders of magnitude. Peaks: 1. You can either call or see a […]

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Domoic Acid Separation with LCMS without Derivatization – AppNote

Several analytical methods have been developed for the quantitative determination of Domoic Acid (DA). However, many of the methods require tedious and labor-intensive derivatization of this compound in order to detect it. A new method based on HPLC and MS detection which does not require derivatization has been developed. You can either call or see […]

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Hydrophobic and Hydrophilic Peptides Analyzed with HPLC – AppNote

Simultaneous Separation using a Capillary Column The studied analytes comprised some very Hydrophobic and Hydrophilic Peptides with diverse isoelectric points (pI) and thus served as useful probes to explore the potential of this App Note in this field of Bioanalysis. The figure shows how these Peptides of widely disparate physicochemical properties could be separated in […]

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Methionine-Enkephalin Analyzed with LCMS – AppNote

Selective Method for an Opiate like Peptide In this AppNote, the developed Method was used to analyze Methionine-Enkephalin (MEK), the resulting Peak shape was Symmetrical. and the Retention time was more than adequate. In addition to the intact peptide, the Gradient method was also designed to analyze its metabolites and enzyme inhibitors. This Gradient Method […]

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Monoclonal Anti-A1-Glycoprotein – AppNote

Glycoprotein Analysis  For the last few decades Glycoproteins have been a subject of interest to biochemists and biologists in many fields. These proteins are found in plasma and other biological fluids and they serve many functions in nearly every physiological process of living organisms. These macromolecules consist of a peptide chain and one or more […]

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Peptide Mixture Separated with HPLC – AppNote

Precise and Fast Equilibration Time HPLC is a technique used for the characterization of Peptides and Reversed Phase HPLC is employed for the initial analysis and final, large scale purification. The first step of the production of Synthetic Peptides usually involves an initial Separation of these compounds from its mixture, on an analytical scale, then […]

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Synthetic Peptide Analyzed with HPLC – AppNote

Peptide Production Confirmation Using the Cogent HPS© C18 An in-process Method for Qualifying a synthetic peptide is shown with 3 samples of tryptic “digests” were injected on the same Column. The Reproducibility from run to run on the samples is excellent giving the user a high level of confidence. The process and Method was further […]

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