UDC-Cholesterol FAQ – MicroSolv Technology Corporation

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Cholesterol is a large molecule, is the Cogent UDC-Cholesterol HPLC column stable as a stationary phase?

The UDC Cholesterol phase has proven to be stable in 0.1% v/v TFA and 0.1% v/v H3PO4 at acid pH’s and up through pH 8.00 with 20mM NH4COOH buffer with NH4OH. A pH in excess of pH 8.00 should be avoided as this phase has a mono dentate (single point) attachment to the silica matrix and an intermediate […]

How do I condition new Cogent TYPE-C™ Columns for use?

For all new Cogent TYPE-C™ HPLC columns with the exception of new Diamond Hydride™ columns, all one needs to do is run 7-10 column bed volumes of your mobile phase through the column at your normal flow rate. Then it is best to inject a known standard and repeat the injections until duplicate chromatograms are […]

Category: UDC-Cholesterol FAQ

Cholesterol is a large molecule, is the Cogent UDC-Cholesterol HPLC column stable as a stationary phase?

How do I condition new Cogent TYPE-C™ Columns for use?

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