Acid Washing Autosampler Vials has Consequences

What are the Advantages and Disadvantages of “Acid Washing” Autosampler Vials? The main reason to “Acid Wash” Autosampler Vials is to reduce basic compound adsorption, due to native silanols onto the glass. Some of the consequences to Acid Washing include time and materials to wash but also you run the risk of Chloride and Sodium […]

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Acid Washing RSA Autosampler Vials has Consequences.

What are the Advantages and Disadvantages of Acid Washing RSA Vials? There is no reason to Acid Wash RSA Vials. The main reason to Wash ordinary Vials is to reduce adsorption due to native silanols on the glass. Since RSA has virtually no silanols, this is not necessary. Some of the consequences to Acid Washing […]

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Add 10 micro molar EDTA to Mobile Phase for Better Peak Shapes.

Metals from glass Bottles and Autosampler Vials or your Instrument can be one cause of poor Peak Shapes for the polyprotic acids and Nucleotides in LCMS. This varies greatly from instrument to instrument so you should identify the source of your poor shapes. One possible solution in this case would be to add 10 micro-molar […]

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Adsorption can Happen Quickly in an Autosampler Vial.

We have shown from previous experiments of how concentration of certain analytes can be adsorbed to glass surfaces over time which is an indicator of potential problems for the laboratory for many other analytes as well. In this experiment, a sample was taken in small timed increments to display the magnitude of potential sample loss […]

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AQ Brand Polypropylene Vial Compared to Market Leading Vial (LCMS).

Many scientists use a very common 300µL plastic (polypropylene) vial for chromatography (HPLC) when they have compounds that stick to glass. With the advancement of LCMS, HILIC and ANP chromatography these vials are not useable because they produce spurious peaks. The peaks are due to common chemicals used to make these vials at a low […]

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AQ™ Brand PP Inserts Study LC-MS Compared to Market Leading PP Vials.

Many scientists use a very common 300µL plastic (polypropylene) vial for chromatography (HPLC) when they have compounds that stick to glass. With the advancement of LCMS, HILIC and ANP chromatography these vials are not useable because they produce spurious peaks. The peaks are due to common chemicals used to make these vials at a low […]

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Are items 95020-45S-T caps and 95010-0CV vials compatible with SPME?

The following two items are compatible with Solid Phase Micro-Extraction (SPME): Catalog # 95020-45S-T: Magnetic Screw Cap for Headspace. Silver, 8mm Center Hole, Silicone Transparent Blue/PTFE White, 45 Shore A, 1.3mm Thick. Precision Threads. 100/PK. Catalog # 95010-0CV: Vials, head space, 10ml, 46 x 22.5mm, screw top, clear borosilicate glass, round bottom. CTC Compliant. Precision threads 100/PK. However, the […]

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Are MicroSolv’s headspace autosampler vials radiation resistant?

Although not engineered specifically for radiation applications, the borosilicate autosampler glass vials should easily withstand typical laboratory sterility radiation exposure in short duration applications such as irradiating biologicals and similar without suffering structural damage. It is important to note that the glass may discolor or may become cloudy. Any MicroSolv vial should not be used […]

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Are polypropylene screw top vials and caps free of metals (sodium, potassium, magnesium, and calcium)?

Polypropylene screw top vials (Item# 9502S-PP-CLEAR) are not tested for metal content. However, we would expect it to be low since metals are not used in the manufacturing process. Since the vial are not tested and after opening the package in environments with metals we can’t guarantee the vials to be metal free. Screw top caps (Item# 9502S-30C-B) are manufactured free […]

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Are RSA Autosampler Vials Pyrogen Free?

The RSA™ or Reduced Surface Activity autosampler vials are Pyrogen Free when manufactured as they are produced sterile and are never touched by “human hands”. They are then packaged, including shrink wrapped in a sterile, class 100,000 clean room environment. We do not classify these vials as Sterile for sale since the packaging is not sterilized […]

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Are RSA Vials Approved by the FDA for Use in Clinical Testing?

The FDA does not “Approve or Disapprove” of Vials for use in Analytical Testing. Therefore any Vial may be used for this purpose to be Compliant with FDA regulations including the RSA™ Vials. However, if Vials are used for Human Clinical Testing or Packaging of Pharmaceuticals, you might need to Validate and get FDA-certified. Please […]

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Are RSA Vials Suitable for Elemental Analysis?

It depends on the analytical technique you are using. Some sample preparations may contain hydrofluoric acid, in which case you can’t use any type of glass. Polypropylene and Teflon containers are common for ICP-MS because of the lower elemental extractables. In addition to Na, B and Si glass leaches out other elements in trace amounts that […]

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Are the AQ™ Advanced Quality brand of LCMS vials compatible with DMSO?

DMSO is not considered reactive towards any polypropylene vial product. Still, one should always be aware of the possibility when using DMSO with laboratory plastic that under some conditions, extractables from the plastic could result and show up as ghost peaks in the HPLC runs. There are many factors that can affect the likelihood of extractables forming, which […]

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Are the Basik™ brand of headspace vials free of endotoxins?

These vials are not tested for the presence of endotoxins but since they are made at 600°C, bacteria or organic matter will not likely exist after manufacturing. However, while the vials are packed directly after cooling, they are not certified to be “untouched by human hands”. More information about Headspace Vials

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Are the MS-compatible polypropylene vials (Cat No. 9532S-MS) hydrophobic or hydrophilic?

The vial is made of MS-compatible polypropylene, which consists only of repeating hydrocarbon monomer units: These polymers have no polar or ionizable groups so the polypropylene vials are hydrophobic. A primary reason one might use these vials is to avoid undesirable properties of hydrophilic silanols on conventional borosilicate glass vials is their hydrophobicity. The silanolic groups […]

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Are the RSA Vial Write-On Patches Ceramic or Painted?

RSA™ vials, both Amber and Clear are supplied with the Write-On Patches are Ceramic and NOT an Epoxy Paint. Lower quality brands vials are supplied with Painted Patches using Epoxy paint, to keep the cost lower but the problem with it is they out-gas while in the packages which causes contamination for some. If you […]

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Are the RSA™ Vials ISO Certified?

No, the RSA™ Vials and Inserts do not come with an ISO Certification. However, the process with which the vials are manufactured is ISO certified.  A certificate of QC Conformance for RSA Vials or Inserts is available upon request. Please contact Customer Service for Assistance to obtain the Certificate. More information about RSA Vials

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Are your HPLC and GC autosampler vials & caps sealed from outside moisture?

The vials & caps are designed for general HPLC or GC applications. Criteria of quality for BASIK™ and MicroSolv™ Brands include suitable fit for the autosampler (not jamming during injections, etc.) and being contaminant-free from the manufacturing process. Quality criteria for AQ™ Brand includes advanced testing by HPLC and LCMS and criteria for AQ™ Brand with […]

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Autosampler Vials sold by MicroSolv will Fit Your Instruments.

All Autosampler Vials sold by MicroSolv will fit commercially available modern HPLC Autosamplers. During the vial manufacturing processes, opto-electronic devices in the Vial converting machines check within fractions of a second to ensure the processed Vials meet the physical specifications (fit and form, dimension, etc.). In the case of a mismatch, the vial is blown […]

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Basic Compound Concentration and Adsorption Study in Autosampler Vials – AppNote

Comparing Cetylpyridinium Chloride Adsorption on RSA v. Standard Autosampler Vials In this study, the importance of concentration is demonstrated when dealing with adsorption during analyses of basic solutes. Using conventional glass of an industry-leading supplier, the basic test analyte Cetylpyridinium Chloride showed notable loss to adsorption with the vial at the lower concentrations. If this […]

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Can a Crimped Cap be Removed from Fused Insert Vials?

The answer depends on the type of De-Capper used. The type with a Rod that pushes downward onto the cap and into the Vial will not be suitable, as this would damage the Vial Insert. There is a type of De-Capper that grabs the Cap from the sides, which resembles pliers and it quite dissimilar […]

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Can AQR™ Autosampler Vial Caps be Autoclaved?

Yes, studies were conducted on our AQR Autosampler Vial Screw Caps (similar to 9512C-1MP-RS AQR™) in which they were autoclaved for about half an hour; a gravity cycle of 250 °F  with sterilization time 15 min and dry time 10 min. The Caps exhibited no Physical Defects such as warping or other deforming and continued […]

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Can I buy Tubes made with RSA Glass?

RSA™ Glass is not available in any Tube Format and at this time, RSA or Reduced Surface Activity Glass is only available in 12x32mm Autosampler Vials. These vials will not release any contaminants into chloroform or any other solvent.

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Changing analyte recovery when using syringe filters

Question: I am doing a sunscreen analysis which requires that the sample solution is filtered. My diluent is 95/5 methanol/DI water and I am using MTC 0.45um nylon syringe filters. One day I observe consistent peak areas but the next day, when using the same solution, the peak areas are higher. What has happened? Answer: The syringe […]

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Effects of washing and re-using autosampler vials on HPLC data

As reported by Thermo, some laboratories may try to save money by washing their used autosampler vials and reusing them. However, these products are designed to be disposable and reuse can cause numerous QC issues, such as contaminant peaks, sample loss, and physical deformations on the glass surface. Here a clear, clean vial shows virtually no peaks in […]

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Effects of washing and re-using autosampler vials on sample loss

As reported by Thermo, laboratories may try to save money by washing their used autosampler vials and reusing them. However, these products are designed to be disposable and reuse can cause numerous QC issues, such as contaminant peaks, sample loss, and physical deformations on the glass surface. Here a variety of vials are tested for sample loss. Only […]

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Effects of washing and re-using autosampler vials on the glass surface

As reported by Thermo Scientific, some budget minded laboratories may try to save money by washing their used autosampler vials and reusing them. However, these products are designed to be disposable and reuse can cause numerous QC issues, such as contaminant peaks, sample loss, and physical deformations on the glass surface. In these microscopy images, you can […]

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Headspace Septa Temperature Performance Specifications

The following table describes the lower and upper recommended limits for temperature conditions that our Headspace Cap Septa can be used in. This does not reflect the safety of the Headspace system (glass vial, septa, cap and gas) used in practice.   Material Temperature Resistant Range (°C)  Silicone/PTFE Seals (completely PTFE Laminated)  -60 – 200  Butyl/PTFE […]

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How are RSA™ vials different from all other autosampler vials?

Using patent pending and proprietary processes of precision manufacturing, RSA™ autosampler vials are superior to all other vials on the market since they are not only made to exact tolerances for your autosampler, they are manufactured for your sample chemistry as well. When ordinary and “LCMS Certified” commercially available autosampler vials are made, they are […]

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How are your vials packaged to ensure no contamination?

All MicroSolv brand vials that carry a CleanPack label on the front side of the PP-Box have been packed in a certified clean-room class 10,000 after having passed the oven at approximately 600°C.  These highly hygienic conditions are a pharmaceutical standard; however, new for chromatography vials. Thus, the consumer can rely on clean, uncontaminated vials […]

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How can I minimize bubble formation with limited volume vials?

Vials with small volume internal ID are designed to allow the user to use most of the sample which may be too low for the injection needle to reach with standard, flat-bottom vials. One drawback, is that filling microvials can lead to bubble formation in some cases requiring the user to physically shake the bubble […]

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How do autosampler vials cause missed injections in HPLC and LCMS?

HPLC instrument manufacturers require “Fit and Form” specifications for vials used in their autosamplers. These include minimum and maximum vial dimensions for height, neck, shoulder and outer dimensional width to prevent jamming the autosampler. If vials are within these specifications jamming problems due to the vials shouldn’t occur. However, most modern autosamplers have an injection […]

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How do autosampler vials cause missed injections?

HPLC instrument manufacturers require “Fit and Form” specifications for vials used in their autosamplers. These include minimum and maximum vial dimensions for height, neck, shoulder and outer dimensional width to prevent jamming the autosampler. If vials are within these specifications jamming problems due to the vials shouldn’t occur. However, most modern autosamplers have an injection sequence […]

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How resistant are the ultra-fine point markers to smearing?

The markers were tested on standard glass autosampler vials and the writing was found to be completely smear-resistant when using a dry cloth, after waiting a short while for the ink to dry.  If you wish to remove the writing however, this can be accomplished with a solvent such as methanol, acetone, or isopropanol.

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