How Much Volume can the 47mm HPLC Membrane Filters Handle?

The 47mm Membrane Filters can be used for vacuum Filtration of Solvents. In most cases, one of these Filters can be used until it is blocked with Particulate Matter at which time, the flow through the membrane will cease. In most applications you will use the Filters for removing small amounts of Particulate Matter from […]

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How Often do You Need to Change Filters for the Filter/Degasser?

The frequency at which the Filter Membrane has to be replaced is dependent on the sample’s volume of undissolved particles. The more particles, the more frequently it has to be replaced and vice versa. For relatively clean samples (e.g. mobile phases) you could change the membrane every 500 mL or so. Filter/Degasser Product Page

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Ideas on Pressure Increases after Each HPLC Injection – Tip

Question: I am injecting cell extract samples on my HPLC instrument and I find that the back pressure increases after each run. Any ideas on what the problem might be? Answer: There are a number of possible causes. First, always be sure to sonicate and filter your mobile phase before introducing to the LC system especially […]

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Should I filter my HPLC Solvents and Buffers before use? – FAQ

The most common cause of excessive back pressure is due to the accumulation of particulates and or permanently adsorbed materials at the top of the HPLC Column. This could be precipitate or un-dissolved particles which can come from your samples or your mobile phase or shredded pieces of pump seals. The best way to minimize […]

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