Citric Acid Analysis using LCMS can be compromised due to the presence of iron in the system. When iron is present, it can cause peak distortion for compounds like this. A possible solution to this problem is to use a small concentration of a chelating agent (EDTA) in the Mobile Phase / Sample diluent to […]
This is most likely due to “channeling” of the flow pattern inside the column. The packed bed uniformity has likely been disrupted and the split peaks and high pressure are indicative of this. In this case, the column will need to be replaced.
Question: I am injecting cell extract samples on my HPLC instrument and I find that the back pressure increases after each run. Any ideas on what the problem might be? Answer: There are a number of possible causes. First, always be sure to sonicate and filter your mobile phase before introducing to the LC system especially […]
The Inline Filter is compatible with any stationary phase in any HPLC Column. The Inline Filter is simply a Stainless Steel (SS) frit encased in a molded, PEEK holder that traps the particles before they get trapped by the frits on the HPLC columns. The Inline Filter can be removed and disposed of keeping the […]
Passivation is a chemical treatment and Purging is a cleaning process. Passivation is a metal finishing process to prevent corrosion and resist rust. In Stainless Steel, (SS) the passivation process uses Nitric Acid or Citric Acid to remove free iron from the surface. This chemical treatment process leads to a protective oxide layer that is […]
Two scenarios are most likely to be the cause of the pressure spikes, but it is not likely due to a defective column that operates normally at any time: 1. An air bubble is present in the system. You can narrow the problem down to the system by trying your column on another HPLC. If […]