Calculating theoretical plates N at half height – How To

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ARTICLES, APPNOTES, FAQ (9187)
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April 14, 2020
Bill Ciccone

ARTICLES, APPNOTES, FAQ / Frequently Asked Questions?? / How To... Information / HPLC Columns FAQ / HPLC Primer / HPLC Suggestions and Information / HPLC Tips and Suggestions

The theoretical plate number N_0.5can be calculated from a chromatogram using the equation below:

N_0.5= 5.54 (t_R/w_0.5)²

Where t_R is retention time and w_0.5is the peak width at half its height. Remember to use the same units (minutes or seconds) for both values (t_R and w_0.5).

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