Choosing The Correct HPLC Column Coupler.

We offer a variety of HPLC Column Couplers. To find the Correct Size, simply match your Tubing Internal Dimension (ID) to the Coupler’s ID. Click HERE for HPLC Unions including Column Coupler Ordering Information The different Coupler Colors correspond with those of the HPLC Tubing Products.

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Do All PEEK HPLC Fittings Fit all Receiving Ports?

No, you must consider if the receiving Port is Metric or English, the Thread Diameter, the “Pilot Length” of the receiving Port, the thread count or pitch and the pressure expected. If you are not sure if your fitting should be used with a receiving port, contact the fitting and receiving Port manufacturer for advice. […]

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Screw Thread Call-Out Definition.

What is a Screw Thread “Call-out” for “English” Fittings? This is a common system for describing the Threading on a Fitting or Screw Cap that uses the English Method. The Thread Call-Out normally consists of two numbers where the first number will refer to the Diameter of the Widest Thread and the second one refers […]

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Solvents and Solutions that Should be Avoided when Using PEEK Fittings or Tubing.

Solvents and Chemicals that should be Avoided when using PEEK Tubing and Fittings include: Nitric acid Sulfuric acid Halogenated Acids, including hydrofluoric, hydrobromic, and hydroiodic acids* Pure Halogenated gases Methylene chloride THF  (Tetrahydrofuran) DMSO  (Dimethyl Sulfoxide) *Note that you can use Hydrochloric Acid, all other HPLC grade solvents and HPLC buffers with most applications of […]

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What Does 316 in 316 Stainless Steel Mean? FAQ

“316” or “Grade 316” is a class of Stainless Steel (SS) used by the Steel Industry to define a specific Quality; it is a Grade of Steel which MicroSolv offers in our Laboratory Tubing, Fittings and HPLC Columns. According to Atlas Steel Australia, “Grade 316 is the Standard Molybdenum-Bearing Grade, second in importance to 304 […]

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What is a Fitting? Defined for HPLC. FAQ

The dictionary defines a fitting as “a small often standardized part used to join other parts together properly.” This is very similar to what plumbers refer as fittings. For the use of chromatographers, fittings consist of nuts with internal and external threads for both high pressure and low pressure connections. Also, unions, crosses and tees […]

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What is the difference between a union and an adapter in HPLC?

The biggest difference is the price where a union is much less expensive than an adapter. For performance considerations, a union will make a connection between tubing and fittings that have the same thread size and thread count configurations. An adapter will connect two different thread sizes and thread count. Click HERE for HPLC Fittings […]

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What is the difference between ferrules made of stainless steel vs. stainless steel/PEEK hybrids?

Stainless steel ferrules are used when: • Absolute maximum operating pressure is required, in excess of 15,000 PSI. • The connection is ‘permanent’; that is, the connection will not be made and broken often, such as when changing columns. The Stainless Steel/PEEK hybrid ferrule (called a high performance ferrule) is: • Typically limited to 15,000 PSI with multi-ferrule […]

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What is the pH range of PEEK laboratory tubing and fittings?

According the some manufacturers of PEEK, the useful pH range of PEEK tubing and fittings for HPLC is 2 – 14 for use with HPLC solvents and buffers. However, I would be very careful with pH 0 to 6 for long term exposure. PEEK is manufactured using Phosphoric Acid and some of the granulate may even still contain […]

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What is the Useful Temperature Range of PEEK Laboratory Products?

The Useful Temperature Range of PEEK related products for Chromatography is up to 100 °C (tubing); up to 125 °C (fittings). PEEK is sometimes rated to 250°C but… It will not be as stable (lower burst pressure for tubing) as it is normally and some Solvents may interact Stronger than under RT with PEEK. PEEK […]

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What makes one HPLC fitting different from another?

Of course, the quality of the manufacturing is important but besides that the most important features of all fittings are: Geometry of the receiving port you are putting the fitting into. Threads on the fitting and receiving port-Count per inch and total length of threads. Material the fitting is made out of for purposes of […]

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What type of fitting is best to use with the Cogent™ HPLC and LCMS columns?

We generally recommend a fitting that can be re-swaged each time one of our columns is removed (both standard 4um and 2.o™ phases) to enable a clean connection when changing HPLC columns. Permanently swaged fittings are not highly recommended, especially for columns with smaller IDs such as 2.1mm,  whereby any dead volume introduced can rapidly cause system performance concerns […]

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Why do some HPLC fittings need ferrules and others do not?

All fittings require some form of “ferrule”. Some fittings have them built in and others require separate pieces. The “head” of the fittings are used to drive the ferrule and tubing into the receiving port and cause a deforming of the ferrules which forces the ferrule to grip on to the tubing and hold it […]

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