Fittings & Tubing for HPLC FAQ – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ
INTERNAL ONLY – CONFIDENTIAL
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SALES POLICIES

Are the Ends of the Pre-Cut Stainless Steel Tubing Polished? FAQ

Yes, the ends of the Pre-Cut SS tubing are polished. They are flat, burr free and have a clean polished finish making them perfect for HPLC. Click HERE for High Pressure Tubing Ordering Information.

Can I Use a PEEK Finger Tight Fitting on Stainless Steel Tubing? Can I Use a SS Fitting on PEEK Tubing in HPLC? FAQ

Yes, you can very effectively use PEEK Fittings on Stainless Steel Tubing as long as you take into account the making Pressure Ratings of the Fitting you are using and the expected Pressures in your System. Generally speaking, a PEEK Fitting is preferred for all HPLC Applications up to 5,000 psi and No, you should […]

Choosing The Correct HPLC Column Coupler.

We offer a variety of HPLC Column Couplers. To find the Correct Size, simply match your Tubing Internal Dimension (ID) to the Coupler’s ID. Click HERE for HPLC Unions including Column Coupler Ordering Information The different Coupler Colors correspond with those of the HPLC Tubing Products.

Contaminants leaching into an analysis, suggestions for troubleshooting.

Have you ever run across a situation where Teflon-like compounds where contaminating an analysis? PFA (PerFlourAlkoxy film, a type of Teflon) is leaching out of something from pipette to HPLC and I have no idea who uses this compound and who doesn’t! This is not easy to answer. In an HPLC system more than one […]

Do All PEEK HPLC Fittings Fit all Receiving Ports?

No, you must consider if the receiving Port is Metric or English, the Thread Diameter, the “Pilot Length” of the receiving Port, the thread count or pitch and the pressure expected. If you are not sure if your fitting should be used with a receiving port, contact the fitting and receiving Port manufacturer for advice. […]

Do I Need a Connector When Using the Cartridge Columns that Use WVS Hardware? FAQ.

The WVS (Whatman Void Sealing) cartridge for Partisil Columns use a unique Sealing System to ensure Compression of the packed bed even if a void develops. All of our WVS cartridge column products require the use of special end fittings (catalog # 66900-WEF) which are used for this purpose. The specialized design of this hardware […]

Do the HPLC Tee Fittings Require Ferrules or Flat-Bottom (Flangeless) Fittings? FAQ

The High Pressure Tee Fittings used in the High Pressure Zones for HPLC require Ferrules. Only the Low Pressure Tee Junctions require the Flat Bottom Fittings. Unions for HPLC Ordering Information

Does MicroSolv Offer a Low Adsorption Tubing Suitable for Protein Analysis? FAQ

The closest tubing we recommend would be low pressure use, FEP or PTFE. PTFE has micro-pores, and proteins may ‘creep’ in there. FEP is preferred because it has the same chemical stability but without the micro-pores. However, you would have to test it for your proteins as we could not guarantee it would be appropriate […]

How Can a Finger Tight Fitting be Producing Band Broadening in HPLC? FAQ

Most Chromatographers may not realize it but if Fittings and Tubing are not Properly Seated and a Perfect Connection is accomplished, a small but important Dead Volume can occur that does not get swept. This Extra Volume can cause Band Broadening or Ghost Peaks or may decrease the Sensitivity of your run by increasing Baseline […]

How Similar Fittings for Chromatography can Differ from Different Brands in HPLC.

Of course, the Quality of the Manufacturing is Important but besides that the Most Important Distinguishing Features are: Geometry of the Receiving Port you are using. Threads Call Out on the Fitting and Receiving Port, count per inch and total length of Threads. Material the Fitting is made out of for purposes of Chemical Inertness, […]

How to connect HPLC columns using Di-Ad Connectors. – Tips

Assembly Instructions for the Di-Ad™ HPLC Column Connectors Push the Direct Adaptive (Di-Ad) Connector into Port while you feel a push back from the spring in the fitting, until you can engage its threads with the threads of the port. Turn fitting clockwise until it is fully seated and finger tighten. No need to over […]

I am Using TFA in HPLC, Will I Have a Problem with my Delrin Type Fittings? FAQ

Delrin is Not Compatible with Trifluoroacetic Acid (TFA). If it comes in contact with your Fittings, your Fitting could become Compromised and your Connection Might Slip and become Less than Perfect. Click HERE to view our HPLC Fittings

Is PEEK Tubing or PEEK Fittings Autoclavable? FAQ

Yes, many Scientists have used PEEK Successfully when Autoclaving PEEK Tubing and or PEEK Fittings using Gamma Irradiation, Heat or Ethylene Oxide sterilization. Click HERE for PEEK Tubing Information

Is Stainless Steel Tubing or Fittings Autoclavable? FAQ

Yes, many Scientists have used 316 Stainless Steel Successfully when Autoclaving Tubing and or Fittings for HPLC using Gamma Irradiation, Heat or Ethylene Oxide Sterilization Methods. Click HERE for Stainless Steel Tubing Information

Screw Thread Call-Out Definition.

What is a Screw Thread “Call-out” for “English” Fittings? This is a common system for describing the Threading on a Fitting or Screw Cap that uses the English Method. The Thread Call-Out normally consists of two numbers where the first number will refer to the Diameter of the Widest Thread and the second one refers […]

Should I Use a 1-Piece Fitting or a 2-Piece Fitting for HPLC? FAQ

This is a Matter of Choice.. The Benefits of a 1-Piece Fitting is that it is Convenient and easy to use. The Benefits of a 2-Piece Fitting is that the Ferrule is the only piece that needs to be Replaced when the unit is Worn Out. For most Applications, they will both provide perfect Connections. […]

Solvents and Solutions that Should be Avoided when Using PEEK Fittings or Tubing.

Solvents and Chemicals that should be Avoided when using PEEK Tubing and Fittings include: Nitric acid Sulfuric acid Halogenated Acids, including hydrofluoric, hydrobromic, and hydroiodic acids* Pure Halogenated gases Methylene chloride THF (Tetrahydrofuran) DMSO (Dimethyl Sulfoxide) *Note that you can use Hydrochloric Acid, all other HPLC grade solvents and HPLC buffers with most applications of […]

What are The Critical Dimensions of Ferrules for Low Pressure Fittings? FAQ

The “Bore Size” of the Ferrules for Low Pressure Fittings is critical for proper connection and for Catalog# 45216-F-ETFE or 45216-F-PTFE it is ID 1.70 ± 0.05 mm. Designed for Use with 1/16th” OD Low Pressure Tubing.

What are The Temperature and Pressure Specifications for Stainless Steel Tubing? FAQ

It is very hard to claim any particular Pressure or Temperature rating for Stainless Steel Tubing used in HPLC Applications without knowing the Specifics of Use as there are many Types of SS Tubing used in the Laboratories. Seamless Welded, Double Welded and Annealed Tubing have different Specs making this a difficult Question to answer. […]

What Does 316 in 316 Stainless Steel Mean? FAQ

“316” or “Grade 316” is a class of Stainless Steel (SS) used by the Steel Industry to define a specific Quality; it is a Grade of Steel which MicroSolv offers in our Laboratory Tubing, Fittings and HPLC Columns. According to Atlas Steel Australia, “Grade 316 is the Standard Molybdenum-Bearing Grade, second in importance to 304 […]

What Does Stainless in Stainless Steel Mean? FAQ

According to Wikipedia, Stainless Steel does not Corrode, Rust or Stain like other Carbon Steel does due to the Chromium added to the Steel. Wikidpedia article about Stainless Steel

What Does the Term 10-32 Mean to a Chromatographer Working with HPLC? FAQ

This Term is a way of Describing the Threading on a Fitting so that it will properly be Matched to a receiving Port such as a Column Connector and a Column. This Match is important for a good Seal and long life of Fittings. Improper Matches will cause the Fittings to Cross Thread and wear […]

What is a Fitting? Defined for HPLC. FAQ

The dictionary defines a fitting as “a small often standardized part used to join other parts together properly.” This is very similar to what plumbers refer as fittings. For the use of chromatographers, fittings consist of nuts with internal and external threads for both high pressure and low pressure connections. Also, unions, crosses and tees […]

What is the best way to attach the inlet of the knitted reactor coil tubing to the outlet of a tee with a PEEK finger tight fitting that will hold high back pressure?

Confirm that there is not an impediment to reasonable flow through the tubing which could increase the back pressure. We recommend using our items 47320-30 and 47320-34 which are High pressure Male nuts and ferrules.

What is the Difference Between a Luer Lock and a Luer Slip Connector?

When using a device such as a Syringe Filter and a Chrom Syringe which both have Luer Slip Connectors on them, the Syringe Filter device will simply slide onto the tip of the Syringe. If it is applied properly (fully placing the device onto the tip of the syringe and a quarter turn while applying […]

What is the difference between a union and an adapter in HPLC?

The biggest difference is the price where a union is much less expensive than an adapter. For performance considerations, a union will make a connection between tubing and fittings that have the same thread size and thread count configurations. An adapter will connect two different thread sizes and thread count. Click HERE for HPLC Fittings […]

What is the difference between ferrules made of stainless steel vs. stainless steel/PEEK hybrids?

Stainless steel ferrules are used when: • Absolute maximum operating pressure is required, in excess of 15,000 PSI. • The connection is ‘permanent’; that is, the connection will not be made and broken often, such as when changing columns. The Stainless Steel/PEEK hybrid ferrule (called a high performance ferrule) is: • Typically limited to 15,000 PSI with multi-ferrule […]

What is the inside diameter ID of standard tubing used in that is most suitable for HPLC mobile phase? FAQ

There really is no standard size and this is truly a preference to meet your method objectives. For HPLC, on the high pressure side, 1/16th inch OD is most commonly used and for the low pressure side either the 1/16th inch or 1/8th inch OD depending on where you want to put the tubing on […]

What is the maximum temperature that the MicroSolv Endure fittings can be used in?

150°C for Endure Carbon PEEK fittings and 100°C for most other PEEK fittings. Click here to view Ordering Information for Male Nuts for HPLC

What is the pH Range of 316 Stainless Steel Laboratory Tubing, Fittings and Sparging Stones? FAQ

The useful pH Range of 316 Stainless Steel Tubing, Fittings and Sparging Stones is 1 – 14. More information about stainless steel tubing

What is the pH range of PEEK laboratory tubing and fittings?

According the some manufacturers of PEEK, the useful pH range of PEEK tubing and fittings for HPLC is 2 – 14 for use with HPLC solvents and buffers. However, I would be very careful with pH 0 to 6 for long term exposure. PEEK is manufactured using Phosphoric Acid and some of the granulate may even still contain […]

What is the pilot length / dimension X depth for end fittings uses to connect HPLC columns? – FAQ

The Stop Depth, Seating Depth, Dimension X or Pilot Length are all words describing the same feature ie: the distance between the end of a ferrule and the terminus of a section of tubing. To optimally work, this tubing must match the inlet and outlet port depths of an HPLC column. Why is optimally seating ferrules […]

What is the pressure capability of the PEEK column coupler?

The HPLC column couplers (cat# 47450-01, 47450-02, 47450-03, or 47450-04) are standard HPLC items and if installed properly while using standard temperatures and normal HPLC solvents, they should be able to withstand pressures up to 10,000 psi. More Information

What is The Pressure Rating for PEEK Fittings & Tubing? FAQ.

Some HPLC PEEK Tubing Connectors / Male Nuts are rated up to 6000psi with Finger Tight Nuts. Another consideration is the burst strength of the PEEK tubing. Below is a table with the recommended ranges of PEEK Tubing.

What is the Pressure Rating of the Column Coupler SKU# 47450-06? – FAQ

The Column Coupler Catalog number 47450-06 is rated for use up to 6,000 psi. Click HERE for Column Coupler Ordering Information

What is the Useful Temperature Range of 316 Stainless Steel Laboratory Products?

The useful Temperature Range of Stainless Steel (SS) related products such as Tubing and HPLC Fittings is up to 240°C.

What is the Useful Temperature Range of PEEK Laboratory Products?

The Useful Temperature Range of PEEK related products for Chromatography is up to 100 °C (tubing); up to 125 °C (fittings). PEEK is sometimes rated to 250°C but… It will not be as stable (lower burst pressure for tubing) as it is normally and some Solvents may interact Stronger than under RT with PEEK. PEEK […]

What makes one HPLC fitting different from another?

Of course, the quality of the manufacturing is important but besides that the most important features of all fittings are: Geometry of the receiving port you are putting the fitting into. Threads on the fitting and receiving port-Count per inch and total length of threads. Material the fitting is made out of for purposes of […]

What type of fitting is best to use with the Cogent™ HPLC and LCMS columns? – FAQ

We generally recommend the Di-Ad Column Connectors instead of a fitting that can be re-swaged each time one of our columns is removed (both standard 4um and 2.o™ phases) to enable a clean connection when changing HPLC columns. The Di-Ad allows proper and easy installation of Cogent columns as well as different columns without the […]

Which Columns are Compatible with the Column Coupler, SKU# 47450-06? – FAQ

This Column Connector can be used with most brands of HPLC columns that have standard end fittings and inlet ports such as all Cogent HPLC Columns. However, it’s not compatible with the deeper Valco™ type column ports such or with Waters® brands of columns. Click HERE for Column Coupler 47450-06 Ordering Information.

Why do some HPLC fittings need ferrules and others do not?

All fittings require some form of “ferrule”. Some fittings have them built in and others require separate pieces. The “head” of the fittings are used to drive the ferrule and tubing into the receiving port and cause a deforming of the ferrules which forces the ferrule to grip on to the tubing and hold it […]