AppNotes Cogent Phenyl Hydride – MicroSolv Technology Corporation

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10 Phenolic Acids Analyzed with LCMS – AppNote

Compounds Shown were Extracted from Commercial Rice. Commercial Rice Extract 3 was spiked with ten standards and analyzed. The Cogent Phenyl Hydride Column was an excellent choice to use for analysis of Phenolic Compounds with Reversed Phase HPLC. The Retention and Separation of all nine available standards was possible using the Method below. Peaks: […]

5 Phenolic Compounds Separated by LCMS – AppNote

Commercial Rice Extract A commercial rice extract was analyzed and peaks were assigned based on retention times and m/z values for the compounds of interest using the Cogent Phenyl Hydride Column . After method validation the developed procedure can be used to evaluate the quality of rice and to develop the best extraction procedure. Peaks: […]

Advantage of Using Cogent TYPE-C Columns for Drugs of Abuse – Extended AppNote

Heroin, MDMA, Morphine, Hydromorphone and 6-MAM Analyzed with LCMS Analysis of Drugs of Abuse encompasses a wide variety of compounds, including controlled substances, prescription drugs, and even over-the-counter formulations. These drugs can be problematic to analyze due to several problems. First, many have similar structures (e.g. morphine and hydromorphone), making adequate selectivity difficult to obtain […]

Analysis of MDMA in Plasma Samples with LCMS – AppNote

Methylenedioxymethamphetamine Analyzed with MS Under the described conditions, MDMA was retained and eluted as a Symmetrical Peak. The Sensitivity of the Method is very good and comparable to that reported with GCMS Detection [1]. Matrix effects were of minor extent and reproducible and hence should not compromise Quantification. The Method can be used for Forensic […]

Cannabis Metabolite Analyzed with LCMS – AppNote

THC Metabolite Found in Urine Matrix Analyzed The extraction procedure using an SPE technique allows for successful detection of this Cannabis metabolite with this Method. It is worth noting that the Retention of this Compound was achieved using Methanol rather than Acetonitrile as the Organic component in the Mobile Phase. Peak: (l)-9-Carboxy-11-Nor-Delta-9-Tetrahydrocannabinol, m/z 345.2060 [M+H]+ […]

Cefprozil E & Z Isomers Analyzed with HPLC – AppNote

LCMS Compatible Method The USP Assay Method for Cefprozil uses a phosphate-based Mobile Phase, which is not compatible with LCMS. In this Method, only Formic Acid is needed in the Mobile Phase. The Method meets the isomer resolution criterion of not less than 2.5 with an average calculated value of 4.4. The tailing factor was […]

Celecoxib Analyzed with HPLC – AppNote

Impurities Method with Improved Peak Shape Celecoxib is Separated from an Impurity in this simple Gradient Method. The Peak shape obtained for this API shows good Peak Symmetry. In addition, the Method shows good repeatability from run to run. The API Retention time %RSD from five runs was 0.025%. Figure A shows the excellent Precision […]

Comparison of Extracts in Jaboticaba Fruit Powder with LCMS – AppNote

Phenolic Compounds Separated with HPLC with LCMS This simple Analytical Method was used to determine the content of very potent compounds present in Jaboticaba extracts and quantified some its Phenolic contents in fermented/non-fermented fruit; estimated by using standard addition. It is important to know the quantitation of compounds before and after fermentation to optimize the […]

Coricidin HBP Day Tablet Analyzed with HPLC- AppNote

Formulation of Guaifenesin and Dextromethorphan HBr This Method shows the separation of two active ingredients in a formulation of Guaifenesin and Dextromethorphan. Dextromethorphan often exhibits tailing in many HPLC methods, but here an excellent Peak shape is observed. This Method is suitable for routine assay of these compounds in combination formulations. A change in wavelength […]

Coricidin HBP Night® Tablet – AppNote

Acetaminophen, Dextromethorphan, and Chlorpheniramine This Method demonstrates the potential of the Cogent Phenyl Hydride 2.o™ Column for analysis of amine-containing analytes in a real formulation. Chlorpheniramine and Dextromethorphan can often be problematic in terms of peak tailing due to the tertiary amines, but this column and method produces excellent peak shapes for both compounds in this […]

Cough Syrup Ingredient Analysis with HPLC – AppNote

Separation of Antitussives, Analgesics, Decongestants, and Preservatives Cold and cough formulations may contain a number of components such as antitussives (Dextromethorphan), decongestants (Pseudoephedrine, Guaifenesin), analgesics (Acetaminophen), and preservatives (Methyl Paraben, Propyl Paraben, Benzoic Acid). The method illustrates good separation between a variety of these compounds, also that symmetric Peak shapes can be obtained in each […]

Cyanidin-3-O-Glucoside Analyzed in Fruit – AppNote

Analysis in Fruit and Vegetable Extracts In this analysis of proprietary fruit & vegetable extracts, the presence of Cyanidin-3-O-Glucoside was confirmed in three out of four extracts. The peaks were Symmetrical and Retained beyond the dead volume. The Method after validation can be used to quality control of commercial fruit extracts as well in studies […]

Dyclonine HCL Analyzed with HPLC – AppNote

A Reproducible Method for Detection of Dyclonine Hydrochloride A rapid, sensitive, and reproducible Method has been developed for detection of Dyclonine HCL. The data below, (overlay of 20 chromatograms ) illustrates how the compound can be adequately Retained and detected using HPLC, with both excellent Peak Shape and run-to-run precision. Using a simple Gradient in […]

Ethambutol Analyzed with LCMS- AppNote

Retention of Hydrophilic Analyte in a Drug Product When Ethambutol is analyzed on many C18 Columns, it strongly interacts with residual Silanol groups in the Stationary Phase. These interactions normally cause excessive Retention and severe Peak broadening. In this LCMS Method, Formic Acid is all that is required for the analysis of charged drugs like […]

Fexofenadine Analyzed with HPLC – AppNote

Simple Isocratic Assay Method without Amine Additives Fexofenadine, antihistamine, has a number of aromatic moieties and the tertiary amine in its chemical structure that can cause Peak tailing issues when using most L11 Columns for Analysis. The USP Assay Monograph for Fexofenadine Tablets uses Triethylamine to reduce tailing. With this Method, Trifluoroacetic Acid is used […]

Fluoxetine Orthogonal Assay Method – AppNote

Simple Methods Without the Use of Ion-Pairing Agents Unlike the USP assay method, Ion Pair Agents are not needed in this application. Both figures show how good Peak symmetry can be achieved either in the Reversed Phase (RP, Figure A) or Aqueous Normal Phase (ANP, Figure B) mode with only Formic Acid as the Mobile […]

Formoterol Fumarate Analyzed with HPLC – AppNote

Forced Degradation Methods Forced degradation studies are useful for developing Stability Indicating Methods of Pharmaceuticals. In this Application Note Separation is obtained between the API and a Degradant formed under two different, strong acidic conditions. The non-degraded Sample produced a sharp, Symmetrical Peak for the API shown in Figure A. In Figure B, strong acid […]

Glycosidic Analyzed with LCMS – AppNote

Cyanidine-3-Hexoside, Luteolin 7-O-Glucoside & Malvidin Dihexoside Observed in Mesquite Flour extracts, these compounds are Glycosidic Flavones and Anthocyanins. The Glycoside Moieties impart polar characteristics to the analytes, which makes them amenable to Retention by the Aqueous Normal Phase (ANP). In this Application Note, the Cogent Phenyl Hydride Column was used to obtain Chromatographic Separation. The […]

Hydrocodone with Acetaminophen Analyzed with HPLC – AppNote

Simple, Robust Assay Method Hydrocodone can yield poor Peak shapes in many conventional Reversed Phase C18 Methods due to its tertiary amine group. The USP Assay Method for Hydrocodone in combination with Acetaminophen uses Triethylamine as a Mobile Phase additive to improve the Peak shape. In this Method however, only Trifluoroacetic Acid is needed in […]

Ketorolac Separation with LCMS – AppNote

Analgesic agent There is a need for analysis of the Active Pharmaceutical Ingredient (API) in Ketorolac Tablet formulations in order to evaluate two different extraction protocols. The use of Ethanol as the extraction solvent was explored initially but DI Water was observed to produce superior extraction efficiency. The chromatograms show how the compound can be […]

Levothyroxine Analysis – AppNote

Superior Resolution, Reproducibility, & Peak Shapes Compared to USP Method The USP assay method for Levothyroxine requires that a resolution of not less than 5.0 must be demonstrated between Levothyroxine and related compound Liothyronine. A chromatogram obtained from following the USP method using a Type-B Silica based L10 Column is shown in Figure B. The […]

Mycotoxins Analyzed with LCMS – Extended AppNote

Ochratoxin A, Zearalenone, Patulin, Aflatoxin B1, B2, G1+G, Fumonisin B1 The detection of Mycotoxins has been an area of increasing focus in ensuring human health due to their high toxicity even at low concentrations. These chemicals are produced by various species of mold, including Aspergillus Penicillium, Paecilomyces, and Fusarium types. Exposure can come from ingestion […]

Peonidin-3-Glucoside Analyzed by LCMS – AppNote

LC-MS method for Anthocyanin- Fruit or Vegetable Extracts In this analysis, the presence of Peonidin-3-O–Glucoside was confirmed in two out of four extracts. The peaks were symmetrical and retained beyond the dead volume. Due to the MS detection and comparison of the retention time, it was possible to find the peak for Peonidin-3-O-Glucoside among many […]

Phenolic Compound Determination with LCMS – AppNote

Compounds in Olive Leaves Extract A commercial Olive Extract was analyzed using the Cogent Phenyl Hydride Column. Only one Oleuropein Peak was detected and it was Symmetrical and well Retained. The results were reproducible (%RSD = 0.2 for Retention Times). According to the literature [1] the extract from Olive leaves should contain additional compounds. To […]

Propantheline Bromide HPLC Methods- AppNote

Orthogonal Methods without Ion-Pairing Agents In the USP assay method for Propantheline Bromide tablets, Sodium Dodecyl Sulfate (SDS) is used as an Ion-Pairing Agent in the Mobile Phase. The long alkyl chain of this additive makes it particularly difficult and time consuming to load and remove from the HPLC column. Is this method, not only […]

Pyrantel Pamoate Analyzed With HPLC – AppNote

Robust Separation with Excellent Peak Shapes The USP Assay Method for Pyrantel Pamoate uses a bare Silica Column with a Mobile Phase of Acetonitrile, Acetic Acid, Water and Diethylamine. Bare Silica Columns are often less Robust than Reversed Phase Columns due to the variable nature of the adsorbed water layer and / or the ion […]

Quinine Sulfate and Impurity Analysis with HPLC – AppNote

API Separation from Impurity without Ion-Pair Reagents The USP Method for Quinine Sulfate requires a Resolution of not less than 1.2 from its main impurity, Dihydroquinine. In the USP Method, the ion pair agents Methanesulfonic Acid and Diethylamine are used in the Mobile Phase. Ion pair agents are often needed to reduce peak tailing of […]

Rice Extract Analyzed with LCMS – AppNote

Content of Phenolic Compounds in Rice Determined by LCMS When commercial Rice Extracts were analyzed, only 6’-O-Feruloylsucrose at 3.01 minutes (Peak not shown for the clarity of the Chromatogram) was found in this particular Rice Extract Sample. Next, the Sample was spiked with the Standards according to the literature referenced below [1]. This Method is […]

Spiked Hesperidin Extract Analyzed with LCMS – AppNote

Eriocotrin, Hesperidin, Naringin, Diosmin In this method, the Hesperidin extract was spiked with three glycosidic flavonoids. The Cogent Phenyl Hydride Column was used to separate the main component of the extract (Hesperidin) and three standards. The MS detection helped to distinguish between the Naringin peak of the standard and a small impurity peak (peak marked […]

Vanillic Acid and 6′-O-Feruloylsucrose Analyzed with LCMS – AppNote

Rice Extract Sample Containing Vanillic Acid and Feruloylsucrose When commercial Rice Extracts were analyzed, only Vanillic Acid (Peak 1) was found in one out of 3 Extracts and 6′-O-Feruloylsucrose (peak 2) was found in all 3 extracts. The results were confirmed by analyzing spiked Rice Extract samples. The analysis was done in a single run […]

β-Carotene Capsule Analyzed with HPLC – AppNote

Separation from Matrix Peaks Beta-carotene may be taken as a dietary supplement in capsule form. In this case, a wide variety of Matrix Peaks were observed in the chromatographic data. It is possible that some of these peaks are various isomers of all-trans ß-carotene or other similar carotenes. In any case, resolution was obtained from […]

Category: AppNotes Cogent Phenyl Hydride