Compounds in Olive Leaves Extract

A commercial Olive Extract was analyzed using the Cogent Phenyl Hydride Column. Only one Oleuropein Peak was detected and it was Symmetrical and well Retained. The results were reproducible (%RSD = 0.2 for Retention Times).

According to the literature [1] the extract from Olive leaves should contain additional compounds. To confirm that the extract doesn’t contain these compounds, spiked Olive leaves extract was analyzed. All these reported, Phenolic compounds were detected and Separated.

Olive Leaves Chromatogram
Olive Leaves Chemical Structures

Peaks:
1. Hydroxytyrosol m/z 177 [M + Na]+
2. Tyrosol m/z 161 [M + Na]+
3. Verbascoside m/z 647 [M + Na]+
4. Luteolin-7-O-glucoside m/z 449 [M + H]+
5. Oleuropein m/z 563 [M + Na]+
6. Apigenin m/z 449 [M + H]+
Not present: Peonidin 3-O-glucoside 463 m/z [M+]

Method Conditions
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
A: DI Water / 0.1% Formic Acid (v/v)
B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:

Time (minutes) %B
0 5
3 15
4 15
6 30
7 30
11 95
14 95
15 5

Post Time: 3 minutes
Injection vol.: 1μL
Flow rate: 0.4mL / minute
Detection: ESI – NEG – Perkin Elmer, Flexar SQ 300 Mass Spectrometer
Sample Preparation: Commercial Olive leaves extract was dissolved in DI Water and spiked at a concentration of 25ppm.
t0: 0.4 minutes

[1] J.E. Hayes, P. Allen, N. Brunton, M.N. O’Grady, and J.P. Kerry, Food Chemistry, 126, (2011) 948–955

Attachment

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