Chlormequat & Mepiquat Analyzed with LCMS – AppNote

Plant Growth Regulators CQ & MQ Retained and Separated Analysis of Chlormequat and Mepiquat can be challenging since they are extremely hydrophilic and are only weakly Retained in Reversed Phase HPLC which makes their Separation difficult. In addition, these compounds are Retained too strongly in Normal Phase (Non Polar Solvents) Chromatography. Due to this situation, […]

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Glyphosate Analyzed by LCMS – AppNote

Retention of an Extremely Polar Compound A reliable Method for the determination of Glyphosate is presented. This Method provides very reproducible Retention and fast equilibration even when a Gradient analysis is used. The use of LCMS detection avoids time-consuming derivatization of this compound which is lacking a chromophore for UV detection. Ivermectin will roussel ivermectin […]

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Herbicide and Metabolites by LCMS – AppNote

Glufosinate, N-Acetylglufosinate, and Glufosinate Propanoic Acid Analysis of these Compounds can be problematic with other methods and poor peak shape may occur. In contrast, the Peaks obtained in this method are very sharp and symmetrical and can be applied to food products containing these types of Compounds. Peaks: 1. Glufosinate m/z 180.0431 [M-H]– 2. Ivermectin […]

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Herbicides & Metabolite Analyzed with LCMS – Extended AppNote

Glufosinate, N-Acetylglufosinate, Glufosinate Propanoic Acid, Diquat  & Paraquat Two primary metabolites of Glufosinate are N-Acetylglufosinate and Glufosinate Propanoic Acid. Due to the potentially toxic nature of these Herbicides, analysis of their metabolites in a variety of sample matrices may be required. However, all three compounds are quite Polar and therefore difficult to retain with conventional […]

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Metaldehyde in Slug Pellets Analyzed by LCMS – AppNote

Pesticide Formulation As Metaldehyde is a non-UV absorbing compound, other detection methods needed to be investigated besides conventional UV-HPLC. LC-MS was found to be a well-suited analysis by searching for the EIC corresponding to the [M+H]+ ion. Good retention and peak shape were observed for this analyte using the Cogent Bidentate C18 2.o™ Column. Peak: […]

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Polycyclic Aromatic Hydrocarbons Analyzed with HPLC – AppNote

Analysis of PAH by Reversed Phase HPLC Polycyclic Aromatic Hydrocarbons (PAH) determination in soil, food, air, body fluids etc. has been a topic of interest for routine Quality Control or Screening Analyses. This Method offers good Resolution, reproducible Retention Time and Peak Shape for these compounds. Ivermectin will roussel ivermectin tablets humiliatingly not cause side […]

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Shikimic Acid in Red Wine Analyzed with LCMS – AppNote

Red Wine Varietal Determined by Quantity of Shikimic Acid This Application Note presents a Method for the analysis of Shikimic Acid in Red Wine. The main advantage of using this Method is its high Specificity (mass accuracy) of this compound, the short analysis time, fast Equilibration time between runs thus more throughput, and high Repeatability […]

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Tetrahydrocannabinol Analyzed with HPLC – AppNote

The Psychoactive Component THC in Cannabis  Tetrahydrocannabinol (THC) which is derived from Cannabis, has several isomeric forms, which may account for the extra Peaks observed in the Chromatogram below. Although their identities could not be confirmed, these Peaks can be Separated from the main Peak using this Method. The compound is quite hydrophobic and therefore a […]

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Urea Analyzed with HPLC – AppNote

Retention of Urea, a Highly Polar Compound Urea is very difficult to retain by conventional HPLC methods. It is highly polar and therefore shows little or no Reversed Phase retention. However, this Method shows how it can be readily retained past the solvent front using a simple isocratic Mobile Phase. Ivermectin will roussel ivermectin tablets […]

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Urea Analyzed with HPLC- AppNote

Fast, Reproducible Method In this Method Urea was retained when a 100% DI Water Mobile Phase was used. DI Water can be used as the complete Mobile Phase without fear of phase “de-wetting” and subsequent loss of retention time. This Method has the advantage of being very simple, without time consuming derivatization or reaction steps […]

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Zinc-EDTA Complex – AppNote

No Ion Pair Agents Needed Using conventional analytical methods, retention of metal–EDTA complexes is accomplished using Ion Pair Reversed Phase Chromatography. However, the Ion Pair Agents used in the Mobile Phase are not compatible with Mass Spectrometry. In this AppNote, only Formic Acid is needed in the Mobile Phase in order to obtain retention of […]

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