Psychoactive Component in Cannabis 

Tetrahydrocannabinol has several isomeric forms, which may account for the extra peaks observed in the Chromatogram below. Although their identities could not be confirmed, these peaks can be separated from the main peak using the Cogent Bidentate C18  Column. The compound is quite hydrophobic and therefore a relatively high organic content is used in the mobile phase to  ensure the retention is not excessive.

Run-to-run precision is very reliable using this Column, as the overlay of five injections demonstrates.

Cannabis Chromatogram
Cannabis Chemical Structure
1. (−)-trans-Δ9-tetrahydrocannabinol
* Unknown Compounds (Suspected Isomeric Forms)

Method Conditions
Column: Cogent Bidentate C18™, 4μm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75 mm
Mobile Phase: 30% DI Water / 70% Acetonitrile / 0.1% Formic Acid (v/v)
Injection vol.: 20 μL
Flow rate: 1 mL/minute
Detection: UV @ 288 nm
Sample Preparation: Δ9-Tetrahydrocannabinol analytical standard solution (1 mg / mL in Methanol, Sigma-Aldrich cat# T4764) was diluted 1:10 with Diluent of 80% Acetonitrile / 20% DI Water.

Note: THC is the psychoactive component found naturally in the cannabis plant. Although still classified as a Schedule I controlled substance at the federal level, several US states now have laws allowing its use for recreational purposes.


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