Bromhexine HPLC Method – Tips & Suggestions

I am currently using the following Method for Analysis of Bromhexine. What Column and / or Alternative Method Conditions can you suggest? Mobile phase: 20:80 Potassium di-hydrogen Phosphate Buffer (Potassium di-hydrogen phosphate 1.0g, add 900ml DI Water, then change pH to 7.0 with 0.5mol / L NaOH Solution, and dilution it to 1000ml with DI […]

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Fosetyl-aluminum HPLC Analysis Tips – Tips & Suggestions

Reversed Phase & Aqueous Normal Phase Method Tips: You can use the Cogent Bidentate C18™ column in reversed phase (RP) with a high water content method. According to a 2014 third-party research article, if the analysis is done by reversed phase an ion pair agent is recommended to increase retention and reduce peak tailing. Here, […]

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How do I condition new Cogent TYPE-C™ Columns for use?

For all new Cogent TYPE-C™ HPLC columns with the exception of new Diamond Hydride™ columns, all one needs to do is run 7-10 column bed volumes of your mobile phase through the column at your normal flow rate. Then it is best to inject a known standard and repeat the injections until duplicate chromatograms are […]

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Why use buffers during analysis and acid buffers such as the phosphoric acid for column storage solvents for Bidenate C18 columns? Will phosphate precipitate in the column?

The Cogent Bidentate C18™ phase can be stored in un-buffered aqueous / organic solvents, but flowing significant bed volumes of un-buffered aqueous will cause a drop in efficiency of approximately 35% before reaching a new steady state. This is why we recommend either acid or basic buffers but not un-buffered eluents during analysis. For storage […]

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