How do I condition new Cogent TYPE-C™ Columns for use?

For all new Cogent TYPE-C™ HPLC columns with the exception of new Diamond Hydride™ columns, all one needs to do is run 7-10 column bed volumes of your mobile phase through the column at your normal flow rate. Then it is best to inject a known standard and repeat the injections until duplicate chromatograms are […]

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Why use buffers during analysis and acid buffers such as the phosphoric acid for column storage solvents for Bidenate C18 columns? Will phosphate precipitate in the column?

The Cogent Bidentate C18™ phase can be stored in un-buffered aqueous / organic solvents, but flowing significant bed volumes of un-buffered aqueous will cause a drop in efficiency of approximately 35% before reaching a new steady state. This is why we recommend either acid or basic buffers but not un-buffered eluents during analysis. For storage […]

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