How do I calculate plug length in Capillary Electrophoresis?

One possible way is to use the “Poiseuille equation” to calculate the plug length: V = (ΔP d4 π t)/(128 η L) where V is volume of the injected plug, ΔP is pressure drop down the length of the capillary (pascals), d is the inside diameter (m), η is the fluid viscosity (Pascal-sec), t is time the pressure is applied (sec), and L is the length of the capillary (m). […]

Read More

How do I remove a blockage in my CE capillary?

If you observe a sudden drop in current, the problem could be due to a particle causing a blockage in the capillary. If this occurs, try flushing the capillary with filtered, CE-grade DI water. To do this, simply inject the water into the capillary with a proper syringe. Inject in the reverse flow direction because particulates […]

Read More

How do I store the Simplus™ bare silica CE capillaries?

For short term reuse, store Simplus™ capillaries at room temperature. Many users simply tape them to the wall near the instrument.  For long term storage and reuse, fill with water and store at room temperature. Before use, store at room temperature. Never refrigerate or heat these before use or during storage. Capillaries for CE Ordering Information

Read More

Simplus Capillary Specifications

The capillary material consists of bare silica with an outer layer of polyimide coating. The polyimide serves to enhance the mechanical properties of the material (a bare silica capillary without polyimide is brittle not physically durable). The O.D. of the capillary is 365um +/- 10um. The applied bending stress values at various bending radii are given in the […]

Read More