CE and CZE FAQ – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ
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Do you offer autosampler vials or caps for use with the Beckman P/ACE MDQ or other CE systems?

No, we do not offer CE vials for any Beckman MDQ or other CE instruments. In this case, it is probably best to buy the vials and especially the caps directly from Beckman.

For the bonded or coated CE capillaries, do you have other lengths?

The bonded or coated capillaries are only available in 1M lengths. The reason is that it is very difficult to push the bonded reagent through a 50um or 75um tube and get proper coating. Ordering Information for Controlled Flow and Zero EOF Capillaries

How do I calculate plug length in Capillary Electrophoresis?

One possible way is to use the “Poiseuille equation” to calculate the plug length: V = (ΔP d4 π t)/(128 η L) where V is volume of the injected plug, ΔP is pressure drop down the length of the capillary (pascals), d is the inside diameter (m), η is the fluid viscosity (Pascal-sec), t is time the pressure is applied (sec), and L is the length of the capillary (m). […]

How do I remove a blockage in my CE capillary?

If you observe a sudden drop in current, the problem could be due to a particle causing a blockage in the capillary. If this occurs, try flushing the capillary with filtered, CE-grade DI water. To do this, simply inject the water into the capillary with a proper syringe. Inject in the reverse flow direction because particulates […]

How do I store the Simplus™ bare silica CE capillaries?

For short term reuse, store Simplus™ capillaries at room temperature. Many users simply tape them to the wall near the instrument. For long term storage and reuse, fill with water and store at room temperature. Before use, store at room temperature. Never refrigerate or heat these before use or during storage. Capillaries for CE Ordering Information

How does reverse EOF differ from normal EOF in Capillary Electrophoresis?

In reverse EOF, reverse polarity is used and therefore negatively charged species (Anions) will migrate to the anode and will be swept past the detector as well by the EOF which is now in the opposite direction. All positively charged species (Cations) which migrate to the cathode may or may not be slowly swept past the detector […]

My electropherogram signals were less intense with the Beckman Coulter PA 800 than with the PA 800 Plus. What happened to the sensitivity?

The Beckman Coulter PA 800 Plus produces more light and therefore the photoreaction will be more intense. The sensitivity of the two systems differs for this reason.

Simplus Capillary Specifications

The capillary material consists of bare silica with an outer layer of polyimide coating. The polyimide serves to enhance the mechanical properties of the material (a bare silica capillary without polyimide is brittle not physically durable). The O.D. of the capillary is 365um +/- 10um. The applied bending stress values at various bending radii are given in the […]

Category: CE and CZE FAQ

Sub Categories:

Buffers for CE FAQ

CE Accessories

Controlled Flow Capillary FAQ

Window Maker FAQ

Zero Flow Capillary FAQ