Headspace Vial FAQ – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ
INTERNAL ONLY – CONFIDENTIAL
ONLINE TOOLS
SALES POLICIES

Are items 95020-45S-T caps and 95010-0CV vials compatible with SPME?

The following two items are compatible with Solid Phase Micro-Extraction (SPME): Catalog # 95020-45S-T: Magnetic Screw Cap for Headspace. Silver, 8mm Center Hole, Silicone Transparent Blue/PTFE White, 45 Shore A, 1.3mm Thick. Precision Threads. 100/PK. Catalog # 95010-0CV: Vials, head space, 10ml, 46 x 22.5mm, screw top, clear borosilicate glass, round bottom. CTC Compliant. Precision threads 100/PK. However, the […]

Are MicroSolv’s headspace autosampler vials radiation resistant?

Although not engineered specifically for radiation applications, the borosilicate autosampler glass vials should easily withstand typical laboratory sterility radiation exposure in short duration applications such as irradiating biologicals and similar without suffering structural damage. It is important to note that the glass may discolor or may become cloudy. Any MicroSolv vial should not be used […]

Are the Basik™ brand of headspace vials free of endotoxins?

These vials are not tested for the presence of endotoxins but since they are made at 600°C, bacteria or organic matter will not likely exist after manufacturing. However, while the vials are packed directly after cooling, they are not certified to be “untouched by human hands”. More information about Headspace Vials

Can I use vial 95025-VN-05C with crimp cap 95025-08-1S in an oven at 105°C for approximately 1 month?

Yes, the product should be acceptable at this temperature and time period.

Headspace Septa Temperature Performance Specifications

The following table describes the lower and upper recommended limits for temperature conditions that our Headspace Cap Septa can be used in. This does not reflect the safety of the Headspace system (glass vial, septa, cap and gas) used in practice. Material Temperature Resistant Range (°C) Silicone/PTFE Seals (completely PTFE Laminated) -60 – 200 Butyl/PTFE […]

How skived vs. cast headspace septa can affect quantitation

In headspace analysis, it is of utmost importance that a tight seal is formed between the septum of the cap and the vial. If there are even small gaps present in the seal, some of the analyte will escape through these gaps and lead to low recovery. In order to avoid problems of this kind, the quality of […]

I heard that silicone rubber cannot be used as an autosampler vial septa at high temperature, is that true?

No, but at very high temperatures, silicone rubber can become hard, almost to the point of being brittle. However, most autosamplers, including Headspace, do not use temperatures high enough to cause this to happen. Extreme Temperature Range -55C to 220C but polypropylene caps structural integrity may be lost at such high temperatures. Click HERE for Headspace […]

In Order to Prevent Gas Venting from a Closed Autosampler Vial Cap, should I use Snap, Crimp, or Screw Cap?

The Crimp Cap provides the best Quality Seal for the longest duration, but only if the Crimping Tool that is used to create the seal is Calibrated Properly and the user performs a quality crimp. To maintain adequate calibration for crimping tools, you should re-calibrate every 10-15 Crimps and verify a Good Seal is produced […]

What is the advantage of using a “Pharmafix” type of septa?

The advantage is that you get a better seal with the “Pharmafix” seal. However, you should be aware that it may not be as inert as a silicone/PTFE septa designed for Headspace is. Click here for more information about Headspace Caps

What is the Difference between 45 Shore A and 55 Shore A Septa?

“Shore” is a measure of the hardness of a material, named after Albert F. Shore. The letter designates the “scale,” which specifies what type of substance is described. In this case, A refers to flexible mold rubbers/plastics. The numbers go from 0 to 100, with 0 being the softest and 100 being the hardest. Hence, cap septa with […]

What is the Maximum Temperature for Headspace Screw Caps with Aluminum Foil Septa? – FAQ

All of our metal magnetic Screw Caps are designed for Headspace analysis and are fabricated for proper fit with the MicroSolv™ Brand Screw Top Headspace Vials. The Maximum Safe Operating Temperature for these Headspace Caps, is 220°C. These Headspace Caps are unique in this aspect as they are allowed to accept this High Temperature due […]

What is the pressure rating of MicroSolv headspace vials and caps?

Headspace vials are designed to withstand high internal pressure, therefore all MicroSolv brand headspace vials have a wall thickness of 1.2mm for added safety, thus it is assured that the vial will not burst. Contrary to popular belief, the seal and not the vial represents the weakest part of the entire system. Under pressure the septa […]

What is the purpose of foil as part of a septa in headspace analysis?

The purpose of the foil is general to be a barrier film. To keep any solvents or gases from affecting the septa in the crimp cap. Click here for more information about Headspace Caps

Which crimp cap is most commonly used for residual solvent testing by the pharmaceutical industry?

The crimp cap with silicone/PTFE septa is most used by the pharmaceutical industry to be in compliance with USP <467>. Our catalog number 95025-09-1S is one of many caps used. Click here for more information about Headspace Vials and Caps

Which crimp caps and septa are suitable for use with Solid Phase MicroExtraction (SPME) vials?

Solid Phase MicroExtraction (SPME) is a technique in which a short fiber coated with a sorbent or polymer quantitatively extracts analytes from a sample matrix. The fiber is then sent to the injector of an instrument such as a Gas Chromatograph (GC) where desorption of the analytes occurs. For this reason, thin septa are highly recommended for SPME […]

Will all MicroSolv headspace crimp caps work for all brands of 20mm, crimp top vials?

Yes, most definitely. However, the crimp top (serum finish) for MicroSolv vials are tested for fit and form to insure precise crimping each and every time. Always be sure to have your crimping tool calibrated every 50 crimps. For more information about calibrating your crimping tool, please contact Technical Service for assistance. Click here for […]

Category: Headspace Vial FAQ