Column Hardware FAQ – MicroSolv Technology Corporation

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Do you have 0.5um titanium or PEEK frits available for the PEEK column hardware?

In addition to the standard 5um stainless steel or PEEK frits, we have 0.5um titanium frits available but not in PEEK. PEEK Column Hardware Product Page

Does MicroSolv Offer TYPE-C HPLC Columns that Do not Have PEEK Components? FAQ

Most of our Cogent TYPE-C™ HPLC Columns are made with State-of-the-art, Column Hardware that contains Stainless Steel Frits that are held to the Column during manufacturing with caps that are made with high quality PEEK. However, with a special order, all Cogent TYPE-C Analytical Columns that have a 2.1 or 4.6mm ID can be manufactured […]

Guidelines for packing resin (packing material or phase) in the PEEK column hardware.

We do not offer any specific SOP for packing columns in PEEK or Stainless Steel column hardware as every packing material or phase is different and needs to be treated and packed differently. This is outside the scope of our expertise. For a packing SOP, you should contact the supplier of your packing material for […]

How do the Terms Nano, Capillary, and Micro-LC Differ in Terms of Column ID and Typical Flow Rates?

The following table shows the flow rate and column ID ranges for the various terms which is generally accepted by chromatographers but definitions may differ from different sources. Term Colum i.d. range (um) Flow rate range (uL/min) Nano 25–150 0.02–1 Capillary 150–500 1–10 Micro 500–1000 10–50

How to Keep Dursan Coated Frits from Biofouling – FAQ

Non surfactant, Brij-35 to Reduce Biofouling When many proteins get trapped on or adhere to Stainless Steel Frits that are hydrophobic like Dursan surfaces, it is a good idea to add Brig-35 to a wash buffer to facilitate removal of very sticky proteins including BSA, mouse IgG and human plasma proteins. Brig-35 will not diminish […]

What are the Dimensions of 4.6mm ID, 5um Frits for PEEK Columns? – FAQ

The Frit itself has an outer diameter of 0.188” and a thickness of 0.062” and is in a PEEK Ring with 0.25” O.D. and 0.181” I.D. The Frit is Press-Fit into the Ring without glues or binders. Click HERE for PEEK Column Hardware Ordering Page.

What are the maximum temperature and pressure specifications for the PEEK column hardware?

The PEEK columns can be used for short-term packing at pressures up to 500 bar (see packing protocol) and 300–350 bar under normal long-term operating conditions. You can use the columns at temperatures up to 150–200 °C, but the maximum recommended operating pressure will be lower at elevated temperatures. In this temperature range, we recommend not exceeding 50–75 bar. Also please […]

What are threading specifications for components of the PEEK columns?

The PEEK column hardware has two specifications for threading: 1. The female outlet threading found on the outside of the end fittings, where the end plugs or extra-column tubing ferrules connect. This threading is standard 10-32 for all items regardless of internal diameter (ID). This accommodates capillary tubing that is 1/16″ outer diameter (OD). 2. The threading that connects the column tube to the […]

What do WCS and WVS stand for in Partisil™ HPLC columns?

WCS = Whatman Compression Sealing hardware WVS = Whatman Void Sealing hardware Void sealing technology allows for easy adjustment of the frit position to compensate for any voids that develop at the head of the column. This can prolong column life in the event that dissolution of a section of the packing bed occurs.

What is the largest I.D. for PEEK columns that you offer?

The largest I.D. available is 7.5mm. The corresponding lengths offered for a column of this I.D. are 30, 50, 100, 150, 250, 300 mm. All of these columns are 5/8″ O.D. PEEK Hardware Product Page

What is the Maximum Pressure Cogent HPLC Columns Will Tolerate? FAQ

Cogent™ HPLC Columns with 4 & 5um particles are rated up to 6,000 psi. However, it is not recommended (by most instrument producers) that analyses are carried out at this high level. No more than 4,500 psi is recommended during the run. For the 2.o™ (2.2um) phases, we suggest not exceeding approximately 9,000 psi in routine usage. […]

What is the pH range of PEEK laboratory tubing and fittings?

According the some manufacturers of PEEK, the useful pH range of PEEK tubing and fittings for HPLC is 2 – 14 for use with HPLC solvents and buffers. However, I would be very careful with pH 0 to 6 for long term exposure. PEEK is manufactured using Phosphoric Acid and some of the granulate may even still contain […]

What is the pressure capability of the PEEK column coupler?

The HPLC column couplers (cat# 47450-01, 47450-02, 47450-03, or 47450-04) are standard HPLC items and if installed properly while using standard temperatures and normal HPLC solvents, they should be able to withstand pressures up to 10,000 psi. More Information

Which size HPLC Guard Column should I use with my 1mm, Cogent 2.o Column?

The 1mm, 2.o Cogent HPLC Columns™ do not have compatible guard columns so we recommend our HPLC PreColumn filters. These disposable inline Filters protect the column from un-dissolved particles do not affect the chromatography. For the 1mm ID column with 2um particle size we recommend item number 49650-005 Click here to view more information about pre-column filters for HPLC

Category: Column Hardware FAQ