How to Keep Dursan Coated Frits from Biofouling – FAQ

Non surfactant, Brij-35 to Reduce Biofouling When many proteins get trapped on or adhere to Stainless Steel Frits that are hydrophobic like Dursan surfaces, it is a good idea to add Brig-35 to a wash buffer to facilitate removal of very sticky proteins including BSA, mouse IgG and human plasma proteins. Brig-35 will not diminish […]

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What are the maximum temperature and pressure specifications for the PEEK column hardware?

The PEEK columns can be used for short-term packing at pressures up to 500 bar (see packing protocol) and 300–350 bar under normal long-term operating conditions.  You can use the columns at temperatures up to 150–200 °C, but the maximum recommended operating pressure will be lower at elevated temperatures. In this temperature range, we recommend not exceeding 50–75 bar. Also please […]

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What are threading specifications for components of the PEEK columns?

The PEEK column hardware has two specifications for threading: 1. The female outlet threading found on the outside of the end fittings, where the end plugs or extra-column tubing ferrules connect. This threading is standard 10-32 for all items regardless of internal diameter (ID). This accommodates capillary tubing that is 1/16″ outer diameter (OD). 2. The threading that connects the column tube to the […]

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What do WCS and WVS stand for in Partisil™ HPLC columns?

WCS = Whatman Compression Sealing hardware WVS = Whatman Void Sealing hardware     Void sealing technology allows for easy adjustment of the frit position to compensate for any voids that develop at the head of the column. This can prolong column life in the event that dissolution of a section of the packing bed occurs.

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What is the Maximum Pressure Cogent HPLC Columns Will Tolerate? FAQ

Cogent™ HPLC Columns with 4 & 5um particles are rated up to 6,000 psi. However, it is not recommended (by most instrument producers) that analyses are carried out at this high level. No more than 4,500 psi is recommended during the run. For the 2.o™ (2.2um) phases, we suggest not exceeding approximately 9,000 psi in routine usage. […]

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What is the pH range of PEEK laboratory tubing and fittings?

According the some manufacturers of PEEK, the useful pH range of PEEK tubing and fittings for HPLC is 2 – 14 for use with HPLC solvents and buffers. However, I would be very careful with pH 0 to 6 for long term exposure. PEEK is manufactured using Phosphoric Acid and some of the granulate may even still contain […]

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Which size HPLC Guard Column should I use with my 1mm, Cogent 2.o Column?

The 1mm, 2.o Cogent HPLC Columns™ do not have compatible guard columns so we recommend our HPLC PreColumn filters. These disposable inline Filters protect the column from un-dissolved particles do not affect the chromatography.  For the 1mm ID column with 2um particle size we recommend item number 49650-005   Click here to view more information about pre-column filters for HPLC

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