Are the MicroSolv Zero and Controlled EOF Capillaries compatible with cartridges of Beckman PA800 & Beckman MDQ?

The MicroSolv Zero and Controlled Flow CE capillaries are definitely compatible with the standard Beckman PA800 and MDQ capillary cartridges. These capillaries have a 360um OD and should have no issue with the standard cartridges. NOTE: These capillaries are not precut to size nor do they have a detection window. This would be the responsibility of the user to […]

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Do you sell CE capillaries with pre-made windows?

We do not sell CE capillaries with a pre-made window for a few reasons; most importantly, the window is fragile often causing damage during shipping. In order to ensure quality capillaries are delivered to our customers, it is best that the end-user makes the window themselves. Another reason we do not offer these capillaries with pre-made windows is […]

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How can I remove polyimide from your capillaries without using heat?

To remove polyimide coating from our Simplus™ brand or Zero™ or Controlled Flow™ capillaries for CE without using heat, it is best to use fuming sulfuric acid at 100°C. You may want to heat the acid on a hot plate in a small shallow plate, using a fume hood and all the safety equipment needed. Then dip the capillary into […]

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How do the CE capillaries provide different amounts of flow?

We coat the bare fused silica with a proprietary polymer that has a large amount of Sulfonic acid groups exposed to the lumen of the capillary. The different flow rates of the columns are due to differing amounts of polymer. The more the polymer the less of a flow. Click here to view Zero and […]

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How does the Zero Flow CE Capillary work?

The Zero Flow™ CE capillary is made from a bare fused silica capillary but has an inner coating which is covalently bonded with linear polyacrylamide (LPA). Since it does not have any ionizable groups it is considered “effectively neutral” and has not charge. Therefore noticeable EOF is not observed when using this capillary even when current is applied across the field […]

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I don’t see buffer coming out of the Zero Flow™ Capillary. Is the capillary defective?

Zero Flow™ Capillaries by MicroSolv are designed to be Electro-Osmotic-Flow (EOF)-free. This is due to a covalently bonded inner coating that eliminates surface hydroxyl group activity responsible for bulk flow or EOF. Therefore it is normal to observe “no eluent” of buffer or any liquid even at high voltages. This can be beneficial because uncontrolled EOF is known to contribute to a lack […]

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What is a Zero EOF CE capillary mostly used for?

There are many uses for Zero EOF in HPCE. One use is to allow analytes to separate based on electrophoretic mobility only. The Zero EOF capillary is an excellent choice for this technique because, regardless of the pH you use to ionize your analytes, the flow will still be zero. Another use is cIEF. Click […]

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