AppNotes Cogent Diol – MicroSolv Technology Corporation

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1,4-Benzodiazepines in Urine Analyzed using RP and ANP – AppNote

Two Types of Retention Modes are Described in this Application Note Four Compounds were well retained and separated in both HPLC Modes shown in Figures A and B; however, the Retention Order was changed depending on the Gradient used. It is worth noticing that the peak intensities were three times higher when using Gradient “A” […]

Advantage of Using Cogent TYPE-C Columns for Drugs of Abuse – Extended AppNote

Heroin, MDMA, Morphine, Hydromorphone and 6-MAM Analyzed with LCMS Analysis of Drugs of Abuse encompasses a wide variety of compounds, including controlled substances, prescription drugs, and even over-the-counter formulations. These drugs can be problematic to analyze due to several problems. First, many have similar structures (e.g. morphine and hydromorphone), making adequate selectivity difficult to obtain […]

Pharmaceuticals in Plasma Analyzed with LCMS – Extended AppNote

Acetyl-L-Carnitine, Codeine, Hydroxybupropion, Isotrentinon, Warfarin One of the most important applications of LC-MS today is the quantitative analysis of Plasma Samples. Pharmaceuticals and their metabolites may need to be monitored in Plasma in fields such as clinical testing, Forensics, and Toxicology studies. Advancements in LC-MS capabilities have enabled new possibilities for these types of analyses, […]

Separation of Polar Solutes with HPLC – AppNote

Niacin, Riboflavin, Folic Acid, Pyridoxine, Metformin, Thiamine The Cogent Diol Column is a good addition to the TYPE-C™ Silica line of HPLC stationary phases. Here, a variety of common polar analytes are well-retained and separated. Peaks: 1. Ascorbic acid 2. Niacin 3. Riboflavin 4. Folic acid 5. Pyridoxine 6. Metformin 7. Thiamine Method Conditions Column: […]

Uric Acid (UA) Metabolites Analyzed by LCMS – AppNote

Allantoin and 6-Aminouracil The presented method for analysis of UA metabolites in human Urine is very simple and does not require Mobile Phase additives or pre-Column derivatization. The peaks are symmetrical and the MS signal is not diminished as is the case when Ammonium Formate or Acetate is used as the Mobile Phase additive. The […]

Uric acid and Metabolites Analyzed by LCMS – AppNote

LCMS Method for Allantoin, 6-Aminouracil, and Uric Acid (UA) The presented method for analysis of UA and its highly polar metabolites is simple and does not require Mobile Phase additives or pre-column derivatization. The Peaks are symmetrical, and the MS signal is not diminished, as is the case when Ammonium Formate or Acetate is used […]

Warfarin, Hydroxybupropion, and Codeine by LCMS – AppNote

Analysis in Spiked Blood Samples In this application, three pharmaceuticals/metabolites are separated using the Cogent Diol 2.o Column. The blood sample illustrates the suitability of the Column for analysis of more complex matrices. Use of Acetone in the Mobile Phase was found to be appropriate for this LC-MS method. As a less expensive and less […]

Category: AppNotes Cogent Diol