EDTA Analysis with HPLC – AppNote

EDTA does not have a significant Chromophore, so to achieve UV Detection, in the Method shown below we used a pre-Column reaction of a Solution of Ferric Chloride with the Sample. The resulting EDTA/Fe3+ has significant UV Absorbance making this a very Sensitive Method. Ethylenediaminetetraacetic acid is extremely difficult to analyze by itself however in […]

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EDTA Analyzed with HPLC – AppNote

Very Sensitive Method for Measuring Ethylenediaminetetraacetic Acid EDTA does not have a significant Chromophore, so to achieve UV Detection, this Method uses a Pre-Column reaction of a solution of Ferric Chloride with the sample. The resulting EDTA / Fe3+ has significant UV Absorbance making this a very sensitive Method. With this Method, Peak Shapes and […]

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Gentian Violet Analyzed with HPLC – AppNote

Separation of Related Compounds Gentian Violet degrades in solution, forming complex set of Degradation products. This Method can easily separate out the various compounds formed in Solution. The figure below shows a USP Standard of Gentian Violet after stressing it in solution thus creating Degradation products. With excellent Selectivity well as great Peak shape this […]

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Synthetic Peptide Analyzed with HPLC – AppNote

Peptide Production Confirmation Using the Cogent HPS© C18 An in-process Method for Qualifying a synthetic peptide is shown with 3 samples of tryptic “digests” were injected on the same Column. The Reproducibility from run to run on the samples is excellent giving the user a high level of confidence. The process and Method was further […]

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