4-(Methylamino)butyric Acid Analyzed with HPLC- AppNote

A gamma-Aminobutyric Acid (GABA) Derivative  This challenging polar compound is easily retained in this simple Method where repeatability of the data is excellent with %RSD<0.1 for the Retention Times. Peak: 4-(Methylamino)butyric Acid Method Conditions: Column: Cogent Diamond Hydride™, 4μm, 100Å Catalog No.: 70000-15P-2 Dimensions: 4.6 x 150mm Mobile Phase: —A: DI Water with 0.1% Formic […]

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Amlodipine, Valsartan, and Hydrochlorothiazide Tablet USP Assay – AppNote

This AppNote demonstrates the USP Method for Amlodipine, Valsartan, and Hydrochlorothiazide Tablets working with the Cogent RP C18. As shown in the Chromatograms, the Peak efficiency is superb and USP Peak tailing guidelines are easily met with the Column. This demonstrates a great alternative Column for easy plug and play with this USP Method. Method […]

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Amoxicillin USP Assay with HPLC – AppNote

Alternate L1 Column used for the USP Assay Method for Amoxicillin This USP Amoxicillin Assay is performed and a five injection overlay shows Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values less than 0.3%. This demonstrates a good alternate Column for […]

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Atorvastatin Tablets Analyzed with HPLC – AppNote

A Robust Method for Analysis of a Hypercholesterolemia Medication A robust and reproducible Method has been developed for this Cholesterol medication.  A commercially available Drug Product was used as well as a reference Standard.  The data below illustrates how the Standards and Drug Product share excellent peak shape using this easy Method. Peak 1. Top […]

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Carboplatin USP assay with HPLC – AppNote

Alternate L8 column used for a similar USP assay method of Carboplatin This similar USP Carboplatin assay is easily performed and the 10 injection overlay below demonstrates run to run consistency and great peak symmetry. The peak efficiency is very good and peak tailing guidelines are easily met with RSD values of less than 1.0%. […]

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Cytarabine Assay with HPLC- AppNote

A Reliable Assay for Detection of a Chemotherapeutic   Cytarabine belongs to the category of chemotherapy called antimetabolites. Antimetabolites are very similar to normal substances within the cell. When these substances are incorporated into the cellular metabolism, they are unable to divide. Antimetabolites are cell-cycle specific and target cells at very specific phases in the […]

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Deoxycholic Acid Analyzed with ELSD- AppNote

An Analysis of a Bile Acid This simple Method shows excellent Peak shape and Reproducibility as shown in the five injection overlay below. As the shape of the molecule is very similar to steroids, the Cogent UDC-Cholesterol™ Column was selected for this Method as it is founded on the ability to retain compounds based on […]

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Desloratadine Assay with HPLC – AppNote

Alternate L1 Column used for HPLC Method of Desloratadine This Desloratadine Assay is easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values below 0.3%. This demonstrates a good alternate Column for your Desloratadine […]

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Dyclonine HCL Analyzed with HPLC – AppNote

A Reproducible Method for Detection of Dyclonine Hydrochloride Click HERE for Column Ordering Information. A rapid, sensitive, and reproducible Method has been developed for detection of Dyclonine HCL. The data below, (overlay of 20 chromatograms ) illustrates how the compound can be adequately Retained and detected using HPLC, with both excellent Peak Shape and run-to-run […]

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Felodipine Extended-Release Tablets USP Assay – AppNote

USP Method for Felodipine ER Tablets This method presents the USP Felodipine Extended-Release Tablet assay using the Cogent RP C18. As shown in the chromatogram with a five injection overlay, the peak efficiency is superb and USP peak tailing guidelines are easily met with the Cogent RP C18™ column.  This demonstrates a great alternative Column […]

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Fipronil Analyzed with HPLC – AppNote

A Reproducible Method for a Phenylpyrazole Insecticide A rapid, sensitive, and reproducible method has been developed for this Insecticide. The data below, (an overlay of 10 chromatograms) illustrates how the compound can be adequately retained and detected using this straightforward method. A phenyl ring in the column stationary phase provides strategic use of π-π Interaction […]

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Fluorouracil Assay Analyzed with HPLC – AppNote

A Reliable Method for a Chemotherapeutic Drug This Fluorouracil Assay is easily performed and demonstrates Run to Run consistency and Precision with Retention Time RSD values below 0.1%. This Method shows reliability for analysis of this Pyrimidine Analog that is an anti-neoplastic anti-metabolite. Peak: Fluorouracil Method Conditions: Column: Cogent RP C18™, 5um, 100Å Catalog No.: […]

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Fluphenazine HCl USP Tablet Assay with HPLC – AppNote

Alternate L7 Column used for the USP Assay Method of Fluphenazine HCl Tablets This USP Fluphenazine HCl Tablet Assay is easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values of 0.2%. This demonstrates […]

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Fluvoxamine Maleate USP Assay with HPLC – AppNote

Alternate L7 Column used for a Similar USP Assay Method of Fluvoxamine Maleate This Similar USP Fluvoxamine Maleate Assay is easily performed and the 5 injection overlay below demonstrates Run to Run consistency and Separation of E/Z Isomers. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values of […]

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Hydrocortisone USP Assay with HPLC – AppNote

Alternate L1 Column used for the USP Assay Method of Hydrocortisone This USP Hydrocortisone Assay is easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values of 0.5%. This demonstrates a good alternate Column […]

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Indole-3-Butyric Acid Analyzed with HPLC – AppNote

A Reproducible Method for Detection of a Plant Hormone Click HERE for Column Ordering Information. A rapid, sensitive, and Reproducible Method has been developed for Analysis of Indole-3-Butyric Acid. The data below, (overlay of 10 chromatograms ) illustrates how the compound can be adequately Retained and detected using a simple Gradient in Reversed Phase HPLC.  […]

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Ivermectin Analyzed with HPLC – AppNote

A Robust Method for Analysis of an Antiparasitic Medication A robust and reproducible Method has been developed for this anthelmintic agent.  The data below (an overlay of 10 chromatograms ) illustrates both excellent Peak Shape and Retention using this easy Method. Peak 1. Ivermectin Method Conditions Column: Cogent Phenyl Hydride™, 4μm, 100Å Catalog No.:  69020-15P Dimensions: […]

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p-Toluenesulfonic Acid Monohydrate Analyzed with HPLC – AppNote

A Reproducible Method for Analysis of an Oxonium Salt A rapid, sensitive, and Reproducible Method has been developed for Analysis of p-Toluenesulfonic Acid Monohydrate. The data below, (an overlay of 5 chromatograms ) illustrates how the compound can be adequately Retained and detected using this straightforward Method. A Phenyl ring in the Column Stationary Phase […]

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PEG 400 Analyzed with ELSD- AppNote

An Analysis of Polyethylene Glycol 400 This ANP Gradient demonstrates an effective Method for PEG 400 analysis in perfusate samples while maintaining excellent linear results (Correlation Coefficient = 0.9998.) As perfusate samples are solutions passed through biological matrixes, these will often have varying levels of salt.  As such, there were no issues observed with these […]

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Ritonavir Analyzed with HPLC – AppNote

A Reproducible Method for Analysis of a Protease Inhibitor Click HERE for Column Ordering Information. A rapid, sensitive, and Reproducible Method has been developed for this Antiretroviral Medication. The data below, (an overlay of 10 chromatograms ) illustrates how the compound can be adequately Retained and detected using this straightforward Method. A Phenyl ring in the Column […]

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Seven Nitroaromatic and Nitroamine Explosives Analyzed with HPLC – AppNote

A Reproducible Method for Detection of a Nitroaromatics Click HERE for Column Ordering Information. The EPA Standard Test Method (8330B) for the analysis of Seven Nitroaromatic and Nitroamine Explosives in Soil with HPLC requires the use of two Reversed-Phase Columns, C18 and CN in series.  These Explosive compounds are notorious for separation difficulty due to the […]

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Sulfonamide Antibiotics Analyzed with HPLC- AppNote

Everyday Reliable & Precise Method This Method offers great Resolution of a mixture of four Sulfa drugs.  These Antibiotics are commonly used in both veterinary medicine and also employed to fight disease in honey bees.  The importance of quantification is vital, as residues are often found in honey samples and are of concern to customers […]

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Tazobactam USP Assay with HPLC – AppNote

Alternate L1 Column used for the USP Assay Method of Tazobactam This USP Tazobactam Assay was easily performed and the five injection overlay below demonstrates Run to Run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met. This Application Note demonstrates a good Alternate Column for your Tazobactam […]

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Tetracycline USP Assay with HPLC- AppNote

USP Method for Tetracycline This USP Tetracycline Assay is performed and a five injection overlay shows run to run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values less than 0.4%. This demonstrates a good Alternate Column for your USP Method.   Peak: Tetracycline Method Conditions: […]

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Ticagrelor Analyzed with HPLC – AppNote

A Reproducible Method for Detection of this Blood Clot Preventative Click HERE for Column Ordering Information. A rapid, sensitive, and Reproducible Method has been developed for analysis of Ticagrelor. The data below, (overlay of 5 chromatograms ) illustrates how the compound can be adequately Retained and detected using a simple Gradient in Reversed Phase HPLC.  […]

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Triton X-100 Assay with HPLC- AppNote

A Sensitive Assay for Detection of a Non-ionic Surfactant  Triton X-100 is used in the sample purification steps of various materials as well as for cleaning apparatus in pharmaceutical manufacturing.  Due to the toxic nature of Triton X-100, a sensitive method for its detection and quantification is very important to identify any residual amounts. This simple […]

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