A Reproducible Method for Analysis of a Protease Inhibitor
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A rapid, sensitive, and Reproducible Method has been developed for this Antiretroviral Medication. The data below, (an overlay of 10 chromatograms ) illustrates how the compound can be adequately Retained and detected using this straightforward Method.

A Phenyl ring in the Column Stationary Phase provides strategic use of π-π Interaction with the Analyte making possible the use of a very simple, Mass Spec-friendly Mobile Phase with Formic Acid as an additive.

10 Injections of Ritonavir

Method Conditions
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No. 69020-10P
Dimensions: 4.6mm x 100mm
Mobile Phase:  (65:35) Acetonitrile / DI Water with 0.1% Formic Acid
Injection vol.: 5μL
Flow rate: 1.0mL / minute
Detection: UV @ 254nm
Sample Preparation: Ritonavir standard prepared as 1.0mg / mL Standard Solution in Mobile Phase
t0: 1.20 Minutes
K: 1.2

Notes: Ritonavir was initially developed as an independent Antiviral Agent but has been shown to possess advantageous properties in combination regimens with low-dose Ritonavir and other Protease Inhibitors. Currently, it is more commonly used as a booster of other Protease Inhibitors. 

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