HPLC Information and Suggestions – MicroSolv Technology Corporation

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How is Pressure Inside Tubing Calculated for HPLC? FAQ

We have a useful Pressure Calculator on our website in which you determine the contribution your tubing ID will bring to the overall back pressure in your system. This can be handy when you are setting up an HPLC system and want to know what ID tubing would be best to use. The lower the […]

My Analyte is Only Soluble at Basic pH. Do I Need to use a Basic Mobile Phase for my Method? – FAQ

Use of a strongly basic Mobile Phase is not recommended for any Silica based HPLC Column because the underlying Silica of the Stationary Phase is prone to dissolution at pH above 8 when the solution has a strong ionic strength. This may seem problematic for some analytes that you want to use in Aqueous Normal […]

Tip for Promoting Analyte Ionization with LCMS – AppNote

Analyte Ionization using L-Tyrosine Figures A and B show the Peak for L-Tyrosine obtained with two LCMS Methods. The analysis shown in Figure B was performed with a stronger ANP Solvent (DI Water) as component A of the Mobile Phase. The obtained Retention time was lower than in Figure A, as was the compound ionization. […]

Will a Different Guard Column Hardware System Different from the Cogent System Affect my Results When Using a Cogent HPLC Column? – FAQ

If the Guard Column is packed well with the same stationary phase as the Analytical column and the Guard System Hardware has no or very little inherent dead volume, it should not affect the results you get compared to the Cogent Guard System. It is important to note that any Guard Column or extra tubing […]

Category: HPLC Information and Suggestions

Sub Categories:

HPLC Column Information

HPLC Tips and Suggestions

Mobile Phase Suggestions

Qualification Information

Trouble Shooting Suggestions

TYPE-C Column Information

How is Pressure Inside Tubing Calculated for HPLC? FAQ

My Analyte is Only Soluble at Basic pH. Do I Need to use a Basic Mobile Phase for my Method? – FAQ

Tip for Promoting Analyte Ionization with LCMS – AppNote

Will a Different Guard Column Hardware System Different from the Cogent System Affect my Results When Using a Cogent HPLC Column? – FAQ

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