HPLC Information and Suggestions – MicroSolv Technology Corporation

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Can I Use Primary Amines With Acetone as the Mobile Phase in LCMS? – FAQ

Yes, you can. Watch out for imines. However, some labs react acetone with primary amines (+heat) to form acetone adducts (imines) in GC and GCMS. This reaction while using acetone as a mobile phase component in LCMS along with primary amines as part of the sample is something to consider. This reaction, according the University of Liverpool requires a primary amine, a ketone […]

How is Pressure Inside Tubing Calculated for HPLC? – FAQ

We have a useful Pressure Calculator on our website in which you determine the contribution your tubing ID will bring to the overall back pressure in your system. This can be handy when you are setting up an HPLC system and want to know what ID tubing would be best to use. The lower the […]

Is the Pre column Filter Compatible with a 2.1mm ID HPLC Column? – FAQ

The Inline Filter is compatible with any stationary phase in any HPLC Column. The Inline Filter is simply a Stainless Steel (SS) frit encased in a molded, PEEK holder that traps the particles before they get trapped by the frits on the HPLC columns. The Inline Filter can be removed and disposed of keeping the […]

My Analyte is Only Soluble at Basic pH do I Need to use a Basic Mobile Phase for my Method? – FAQ

Use of a strongly basic Mobile Phase is not recommended for any Silica based HPLC Column because the underlying Silica of the Stationary Phase is prone to dissolution at pH above 8 when the solution has a strong ionic strength. This may seem problematic for some analytes that you want to use in Aqueous Normal […]

Tip for Promoting Analyte Ionization with LCMS – AppNote

Analyte Ionization using L-Tyrosine Figures A and B show the Peak for L-Tyrosine obtained with two LCMS Methods. The analysis shown in Figure B was performed with a stronger ANP Solvent (DI Water) as component A of the Mobile Phase. The obtained Retention time was lower than in Figure A, as was the compound ionization. […]

What can I do to keep my Cogent™ TYPE-C Columns Free of Adsorbed Contaminants? – FAQ

Depending on the analytes you use with the Column, there are a number of strategies you can try: • If your HPLC pump and system has more than two solvents, you could include a third line with a cleaning solvent. Then in the injection sequence you could insert a wash step which uses only the […]

Why do I see Large Intermittent Pressure Spikes in HPLC runs, can it be a Defective Column? – FAQ

Two scenarios are most likely to be the cause of the pressure spikes, but it is not likely due to a defective column that operates normally at any time: 1. An air bubble is present in the system. You can narrow the problem down to the system by trying your column on another HPLC. If […]

Will a Different Guard Column Hardware System Different from the Cogent System Affect my Results When Using a Cogent HPLC Column? – FAQ

If the Guard Column is packed well with the same stationary phase as the Analytical column and the Guard System Hardware has no or very little inherent dead volume, it should not affect the results you get compared to the Cogent Guard System. It is important to note that any Guard Column or extra tubing […]

Category: HPLC Information and Suggestions

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