Can I Use Primary Amines With Acetone as the Mobile Phase in LCMS? – FAQ

Yes, you can. Watch out for imines. However, some labs react acetone with primary amines (+heat) to form acetone adducts (imines) in GC and GCMS. This reaction while using acetone as a mobile phase component in LCMS along with primary amines as part of the sample is something to consider. This reaction, according the University of Liverpool requires a primary amine, a ketone […]

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How is Pressure Inside Tubing Calculated for HPLC? – FAQ

We have a useful Pressure Calculator on our website in which you determine the contribution your tubing ID will bring to the overall back pressure in your system. This can be handy when you are setting up an HPLC system and want to know what ID tubing would be best to use. The lower the […]

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Tip for Promoting Analyte Ionization with LCMS – AppNote

Analyte Ionization using L-Tyrosine Figures A and B show the Peak for L-Tyrosine obtained with two LCMS Methods. The analysis shown in Figure B was performed with a stronger ANP Solvent (DI Water) as component A of the Mobile Phase. The obtained Retention time was lower than in Figure A, as was the compound ionization. […]

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