In the chart below, tR is the retention time and tM is the hold-up time.
Definition: the hold-up time is the time of an analyte (small molecule) which completely penetrates the pores and which is not retained at all by the stationary phase.
In the chart below, tR is the retention time and tM is the hold-up time.
Definition: the hold-up time is the time of an analyte (small molecule) which completely penetrates the pores and which is not retained at all by the stationary phase.
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