Adjusted retention time hold up time – Primer – MicroSolv Technology Corporation

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ARTICLES, APPNOTES, FAQ (9438)
- AppNotes Library (1910)
  - AppNotes Alternate Generic Methods (28)
  - AppNotes ANP/HILIC Methods (199)
  - AppNotes Listed by Column (492)
    
    AppNotes Cogent Biphenyl (1)
    
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    AppNotes Cogent RP C8 (8)
    
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    AppNotes TYPE-C Columns (397)
    
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    AppNotes Cogent Bidentate C18 (96)
    
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    AppNotes Cogent Bidentate C8 300 (5)
    
    AppNotes Cogent Diamond Hydride (181)
    
    AppNotes Cogent Diol (7)
    
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    AppNotes Cogent UDC Cholesterol (19)
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    AppNotes Proteins Peptides (9)
  - AppNotes Normal Phase (4)
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- Autosampler Vials Information (323)
  - Headspace Vials & Caps (27)
  - HPLC & GC Standard Vials Caps & Inserts (66)
  - Plastic Vials (28)
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- Chromatography Accessories & Supplies Information (473)
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    Beta Tool Information (2)
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- Frequently Asked Questions?? (3461)
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    MicroSolv Autosampler Vials FAQ (118)
    
    RSA Vials FAQ (112)
    
    U-2D Sample MGT FAQ (57)
    
    Vials & Caps General FAQ (147)
  - CE and CZE FAQ (179)
    
    Buffers for CE FAQ (18)
    
    Controlled Flow Capillary FAQ (33)
    
    Window Maker FAQ (26)
    
    Zero Flow Capillary FAQ (30)
  - Equipment FAQ (98)
    
    Knitted Reactor Coils (14)
    
    Tips Liquid Handling (7)
  - Filtration FAQ (177)
    
    Chrom Syringes FAQ (7)
    
    Cogent Column Filters – FAQ (24)
    
    Filter Degasser FAQ (23)
    
    Syringe Filters FAQ (44)
  - Fittings & Tubing for HPLC FAQ (120)
  - HPLC Columns FAQ (820)
    
    2.o™ HPLC Columns FAQ (99)
    
    Amide FAQ (8)
    
    Bidentate C18 FAQ (26)
    
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    Column Hardware FAQ (74)
    
    Diamond Hydride FAQ (84)
    
    Diol FAQ (6)
    
    Guard Columns FAQ (27)
    
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May 15, 2013
Bill Ciccone

ARTICLES, APPNOTES, FAQ / HPLC Primer / HPLC Suggestions and Information / HPLC Tips and Suggestions / Trouble Shooting Suggestions

In the chart below, t_R is the retention time and t_M is the hold-up time.

Definition: the hold-up time is the time of an analyte (small molecule) which completely penetrates the pores and which is not retained at all by the stationary phase.

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