Can I use 4um guard columns with the 2.o™ analytical columns?

We would recommend against using different particle sizes and stationary phasess for guard and analytical column combination. That being said, the Cogent 2.o™ columns are valuable so it may be worth trying and seeing if the guard column does or does not have an adverse effect on efficiency in the method. If the purpose of using a […]

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How do I connect a guard column with my analytical column?

A convenient way to easily connect the analytical column to the guard column holder is to use our column couplers (See link below).  Alternately you can use PEEK tubing and ferrules instead. This setup is less ideal though because cutting tubing can be difficult to get perfect cuts and poor tubing connection can cause band broadening. The column […]

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Is your guard column holder supposed to close all the way?

When you insert the guard column cartridge and close the holder, you may notice that the holder does not appear to close all the way. This is normal and within the design specifications of the unit. With the cartridge placed in the holder, simply give the holder a quarter turn with a wrench and this […]

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What is the maximum pressure of your guard columns?

The maximum pressure is the same as the analytical columns. The standard 4um columns are rated up to 6000 psi but no more than 4500 psi is recommended during the run. The 2.o™ phases are suggested at no more than 9000 psi.  A PEEK column coupler will suffice unless you are using UHPLC, which is […]

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