Are your plastic coated glass bottles for HPLC Mobile Phases Autoclavable?

No, they are not. If the bottles were to be autoclaved, it could cause some discoloration because of the plastic coating. In addition, autoclaving by heat will melt the plastic, and autoclaving by gas may discolor and harden the plastic. In either event, it is not recommended. To sterilize, we recommend using the non-coated bottles with […]

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Bubbles Forming When Using Inlet Filters for HPLC Mobile Phases.

Bubbles Can Form in an HPLC Mobile Phase. When an HPLC pump is active, a vacuum is present in the tubing and any Filter that is connected to the pump. If the liquid cannot flow fast enough, an Air Bubble will result which is commonly called “cavitation”. This cavitation can occur for a variety of […]

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Can I Use Acetone as a Mobile Phase Solvent in ANP? – FAQ

If you are using LCMS, the simple answer is yes but pH is also something to consider. Acetone has a high UV cutoff and therefore can be a problem for UV detection. However, this is not a problem when using LCMS or ELS detectors such as the Corona CAD. You can use Acetone to replace Acetonitrile […]

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Can I use benzene as a mobile phase solvent in HPLC?

Benzene can be used as an HPLC solvent in theory. It is compatible with standard HPLC instrumentation and will not damage an HPLC column. However, we would not recommend using this solvent for two main reasons. First, it is strongly UV-absorbing so would not be suitable for UV-based detection methods. Second, it is a known carcinogen and therefore […]

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Can I use SS tubing with my MicroSolv sparging stone?

Our sparging/filter stones are designed to work with low pressure tubing such as PTFE, FEP, ETFE, or PFA. We offer two types of sparging devices. One type is will require the tubing to be inserted and screwed into the sparging stones. Inside there are small, sharp chads or barbs that grips into the soft tubing holding it in place. If tubing […]

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How much pressure can your mobile phase handling reservoirs handle?

These mobile phase reservoir bottles are not pressure rated and are not intended to be pressurized. Normally the bottle stands above the HPLC-system and this provides enough pressure for the solvent to flow where it is intended to flow. We can not recommend to pressurize the reservoir bottles either coated or non-coated.

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How Much Volume can the 47mm HPLC Membrane Filters Handle?

The 47mm Membrane Filters can be used for vacuum Filtration of Solvents. In most cases, one of these Filters can be used until it is blocked with Particulate Matter at which time, the flow through the membrane will cease. In most applications you will use the Filters for removing small amounts of Particulate Matter from […]

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How Often do You Need to Change Filters for the Filter/Degasser?

The frequency at which the Filter Membrane has to be replaced is dependent on the sample’s volume of undissolved particles. The more particles, the more frequently it has to be replaced and vice versa. For relatively clean samples (e.g. mobile phases) you could change the membrane every 500 mL or so. Filter/Degasser Product Page

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How to Avoid Microbial Growth in HPLC Mobile Phases.

Bacteria can be a problem in the case of Aqueous Solvents. If present, they can create problems for an instrument’s operation. There are a few things you can do to avoid their formation in your mobile phase. One is to add an acid such as 0.1% Formic Acid. Many HPLC methods may use an acidified […]

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Should I use methanol as the organic solvent for ANP in HPLC?

Methanol is generally not recommended for most ANP applications however a few scientists have seen success with it. Instead, it is best to use polar aprotic solvents such as acetonitrile. If using ELSD or LCMS, acetone can also be a good alternative to acetonitrile as well. However, because of its high UV cutoff, it is generally […]

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The pH range of common buffers for HPLC.

The following table shows a list of common buffers and their working buffer ranges: Buffer pH Range  trifluoroacetic acid (TFA)  1.5-2.5  phosphoric acid/monobasic phosphate (pKa 1)  1.1-3.1  formic acid/formate  2.8-4.8  acetic acid/acetate  3.8-5.8  mono/dibasic phosphate (pKa 2)  6.2-8.2  ammonia  8.2-10.2  1-methylpiperidine  9.1-11.1  triethylamine  10.0-12.0 As for concentration, about 5mM is suitable for Cogent TYPE-C Silica™ […]

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What are the dimensions and specifications of the 10L reservoir bottles?

Our 10L (10,000ml) solvent reservoir or mobile phase storage bottles have the following specifications: Material:                   Borosilicate Glass Code 7740 Safety Coating:        Proprietary epoxy plastic Capacity:                  10000 mL Bottle Style:              Storage Neck Style:               Wide Mouth Top Style:                 Threaded Cap Style:                 Plug Seal Cap Material:            Polypropylene Bottle Shape:           Cylindrical Cap Color:                Orange Thread Finish:          45 GL Graduation Range:  2000-9000 […]

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What is an “inlet sinker” in HPLC?

I am not exactly sure, but “inlet sinker” has been used as another name for stainless steel sparging stones or stainless steel inlet filters. This term may be used since the stones are heavy and sink to the bottom of the mobile phase inlet bottles. Click HERE for HPLC Mobile Phase Filtering Ordering Information Including Sparging Stones

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What is the pressure limit of the stainless steel sparging stones?

All the sparging stone products are rated to 1000 bar. However it is important to note that the tubing used and the connection of the tubing to the device may not perform at this pressure rating. The pressure limit of sparging stone is not for the filters/spargers, but for the connections. Mobile Phase Filters Ordering Information

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