If I do not degas my mobile phase for HPLC, what are the consequences?

Solvents that are exposed to the atmosphere have the ability to absorb compressible CO2 and Oxygen. When your HPLC has low back pressure, the actual linear flow of materials could be different then when you have higher back pressure. This is due to the compressibility of air v. the compressibility of gas. This means that […]

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