Alternate Column used for the USP® Assay Method of Tazobactam

This USP® Tazobactam Assay is easily performed and the five injection overlay below demonstrates Run to Run Precision. The Peak Efficiency is very good and USP Peak Tailing guidelines are easily met with RSD values well below 2.0%. This demonstrates a good Alternate L1 Column for your Tazobactam USP Method.

Peak:

Peak

Tazobactam

Method Conditions:
Column: Cogent Hyper-Similars C18™, 5um, 150Å
Catalog No.: 62518-25P
Dimensions: 4.6mm x 250mm
Mobile Phase: Add 30 mL of Acetonitrile to Buffer, mix, and pass through a 0.2-μm pore size filter.  
Buffer: 1.32g of Dibasic Ammonium Phosphate in 1000mL of DI Water. ( pH adjusted to 2.5 with 5% (v/v) Phosphoric Acid )
Injection vol.: 20μL
Flow rate: 1.0mL / minute
Detection: UV @ 210nm
Sample Preparation: Tazobactam (0.5mg) in Mobile Phase

Note 1: Tazobactam is is a Penicillanic Acid Sulfone derivative and an Antibiotic of the Beta-lactamase inhibitor class.  This compound prevents the breakdown of other Antibiotics by Beta-lactamase enzyme producing organisms. 

Note 2: The Cogent Hyper-Similars C18™ HPLC Column performs very similarly to the ThermoFisher® Hypersil BD® product line.