The Inline Filter is compatible with any stationary phase in any HPLC Column.
The Inline Filter is simply a Stainless Steel (SS) frit encased in a molded, PEEK holder that traps the particles before they get trapped by the frits on the HPLC columns. The Inline Filter can be removed and disposed of keeping the column frits fresh and clean.
One compatibility concern to keep in mind is that the internal flow path of the Inline Filter is designed for 4.6mm ID Columns. By placing a 4.6mm ID Inline Filter with 2.1mm ID Column there could be band broadening. The analyst should consider this flow path change and their current system tubing with what the Method System Suitability requirements are. Since most analysts do not normally change their entire system tubing to run 2.1mm Columns and use standard ID tubing like 0.005” ID, in this case the Inline Filter would likely would work well.
If one is using a separate HPLC instrument hyphenated with an MS the system tubing is usually .005” ID and may be okay whereas if using an LCMS unit that is designed for LCMS, the system tubing might have a smaller ID, where this Inline filter may not be best suited.
One additional factor that might make this Inline Filter non-compatible for some users would be if they are observing issues with nucleotides or other compounds that interact with Stainless Steel. If metal interaction compatibility is an issue, the Inline Filter element is made with Stainless Steel and may cause analyte adsorption/loss and/or poor peak shape. When analyzing biological samples, one might consider the benefits of employing a Titanium Inline Filter. Titanium offers a higher corrosion resistance compared to SS and is also more biocompatible for protein analysis.