Use non surfactant, Brij-35 to reduce biofouling
When many proteins get trapped on or adhere to stainless steel frits that are hydrophobic like Dursan surfaces, it is a good idea to add Brig-35 to a wash buffer to facilitate removal of very sticky proteins including BSA, mouse IgG and human plasma proteins.
Brig-35 will not diminish or change the effectiveness of the Dursan surface. It is important to work in low concentrations of any ionic polymer to minimize biofouling due to surfaces.
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