The UDC Cholesterol phase has proven to be stable in 0.1% v/v TFA and 0.1% v/v H3PO4 at acid pH’s and up through pH 8.00 with 20mM NH4COOH buffer with NH4OH.

A pH in excess of pH 8.00 should be avoided as this phase has a mono dentate (single point) attachment to the silica matrix and an intermediate ester link in its structure. For higher pH’s the Cogent Bidentate C18™ is recommended.

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