Isocratic Method Retains Benzophenone with Efficiency and Precision

Benzophenone, a hydrophobic non ionic compound, was selected to illustrate the excellent Reversed Phase HPLC capabilities of this Cogent TYPE-C Silica Hydride Stationary Phase.

The Reproducibility, Efficiency and Peak Symmetry of this Method is excellent, as presented in each figure below, which represents ten consecutive injections with two different Mobile Phases. When the percent of Acetonitrile increases (from 70% in Solution A to 80% in Solvent B) the Retention Time of Benzophenone decreases.

Method Conditions
Column: Cogent Bidentate C18™, 4μm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase:
A: 70:30 Acetonitrile / DI Water
B: 80:20 Acetonitrile / DI Water
Injection vol.: 1μL
Flow rate: 0.5mL / minute
Detection: UV @ 254nm
Sample Preparation: Benzophenone, 0.1mg in the A and B Mobile Phases.

Note: Benzophenone has a role as a photosensitizing agent and a plant metabolite, an additive in flavorings or perfumes for “sweet-woody-geranium-like notes, extensively as photo-physical probes to identify and map peptide–protein interactions. Benzophenone appears as white solid with a flowery odor. May float or sink in water.


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