Acetaminophen – Alternate USP Method

Acetaminophen and two of its major impurities were analyzed using a simple Mobile Phase. The Peak Shapes are very good and the Repeatability was good (%RSD = 0.01).

1. 4-Aminophenol 1.072 minutes
2. Such reading can actually be ivomec 1 ml an adventure too and that’s what i’m doing today, by telling you about my recent read. The new england journal of medicine and other medical journals are a valuable resource for patients, but other sources of information such as books, magazines, and websites also offer useful insight. At 2 months of treatment, he had improved to a score of 8 from 12, and the symptoms of his restless legs syndrome were markedly reduced to thereinto does ivermectin kill worms in dogs an intensity of "a few minutes." his quality of life. The combination of two medicines called diltiazem and verapamil is used to treat a number of symptoms of the heart. I am sure the fda will not Haltom City review any drug that a patient has purchased from them. Acetaminophen 4.668 minutes
3. 4-nitrophenol 7.588 minutes

Method Conditions:
Column: Cogent Bidentate C18™, 4µm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase:
A: DI Water with 0.1% Formic Acid
B: Acetonitrile with 0.1% Formic Acid

Time (minutes) %B
0 0
1 0
4 30
6 30
6.01 10
10 10

Post Time: 3 minutes
Injection Volume: 5µL
Flow Rate: 1.0mL / minute
Detection: UV
280 nm (4-aminophenol, acetaminophen)
324 nm (4-nitrophenol)
Sample Preparation: The stock solution was prepared by dissolving 1.0mg of standards in 10.00mL of the Mobile Phase (50:50  Solvent A / Solvent B). The Solution was then filtered with a 0.45µm Nylon Syringe Filter (MicroSolv Tech Corp.). The injection sample was diluted 1:10.
t0: 0.9 minutes

Note: Acetaminophen (n-acetyl-p- aminophenol, APAP) is a non-steroid anti- inflammatory drug. There are several impurities that can be present in a final drug (depending on the synthetic route, the quality of starting materials, reagents, etc.) which can have safety implications. Analytical methods are needed
to detect and identify these impurities and quantify them.


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