Gradient Separation of Acetaminophen, Caffeine, and Butalbital
In this Method, a simple Reversed Phase gradient is used to separate the three components of a Fioricet tablet. Figure A shows the five-run overlay obtained from the gradient. Figure B shows a “zoomed in view” so that the Butalbital Peak can be seen clearly.
Column: Cogent Bidentate C18™, 4µm, 100Å
Catalog No.: 40018-15P
Dimensions: 4.6 x 150mm
—A: DI Water / 0.1% Formic Acid
—B: 97% Acetonitrile / 3% DI Water / 0.1% Formic Acid
Injection vol.: 10µL
Flow rate: 0.8mL / minute
Detection: UV @ 240nm
—Stock Solution: Fioricet tablet was ground, added to 10mL volumetric flask and diluted with 50:50 Solvent A / Solvent B mixture. The flask was vortexed 5 minutes and a portion of the solution was filtered with a 0.45µm Nylon Syringe Filter.
—Working Solution: 10µL of the stock was diluted with 990µL of 50:50 Solvent A / Solvent B mixture.
t0: 2.2 minutes
Note: Fioricet/Esgic is a combination of Acetaminophen, Caffeine, and Butalbital. It is used to treat tension headaches, muscle contraction headaches, and migraines.
No 137 Fioricet Analyzed with HPLC pdf 0.9 Mb Download File