Atorvastatin HPLC Method Transfer to UHPLC – AppNote

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May 22, 2014
Bill Ciccone

AppNotes Cogent Bidentate C18 / AppNotes Library / AppNotes Pharmaceuticals / AppNotes Reversed Phase Methods / ARTICLES, APPNOTES, FAQ

Transfer to “near-UHPLC” 2.2um Column, for the API Atorvastatin

This Application Note demonstrates how data obtained on a 4μm Cogent Bidentate C18 Column (Figure B) can be adapted for a 2.2μm Column (Figure A). The retention times of both analytes are very comparable in each figure. This Method produces excellent Separation of the API and its main acid degradant and is LCMS compatible which can be used in clinical applications involving plasma samples.

Peaks:
1. Atorvastatin
2. Atorvastatin Lactone

Method Conditions
Columns:
—Figure A: Cogent Bidentate C18 2.o™, 2.2μm, 120Å
—Figure B: Cogent Bidentate C18™, 4μm, 100Å
Catalog Nos.:
—Figure A: 40218-05P-2
—Figure B: 40018-05P-2
Column Dimensions: 2.1 x 50mm
Mobile Phases:
—A: DI Water / 10mM Ammonium Acetate
—B: 90:10 Acetonitrile / DI Water / 10mM Ammonium Acetate
Gradients:

Time (minutes)	%B
0	40
1	100
6	100
7	40

Flow rates: 0.3mL / minute
Detection: UV @ 248nm
Sample Preparation: 40mg strength Lipitor® tablet (Atorvastatin) was ground and added to a 50mL volumetric flask with a portion of Solvent B diluent. The solution was sonicated 10 minutes and diluted to mark with Solvent B. It was then filtered through a 0.45μm Nylon Syringe Filter (MicroSolv Tech Corp.). The filtrate was diluted 4x in a diluent of 50:50 Solvent B / 1N HCL. It was heated in a dry bath for 10 minutes at 85°C.

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