AppNotes Reversed Phase Methods – Page 4 – MicroSolv Technology Corporation

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Valsartan and Hydrochlorothiazide Analyzed with HPLC – AppNote

Improved Gradient Method with Faster Equilibration The USP Assay Method for Valsartan in combination with Hydrochlorothiazide features a 27 minute gradient with a 13 minute re-equilibration for a total run time of 40 minutes. In this method, the run time was a quarter of the USP method, and the Column equilibrates much faster when gradients […]

Vanillic Acid and 6′-O-Feruloylsucrose Analyzed with LCMS – AppNote

Rice Extract Sample Containing Vanillic Acid and Feruloylsucrose When commercial Rice Extracts were analyzed, only Vanillic Acid (Peak 1) was found in one out of 3 Extracts and 6′-O-Feruloylsucrose (peak 2) was found in all 3 extracts. The results were confirmed by analyzing spiked Rice Extract samples. The analysis was done in a single run […]

Vitamin K Isomers Analyzed with HPLC – AppNote

Phytonadione Separation by Shape Selectivity In this Method, we separate the two E and Z isomers of Phytonadione on the basis of shape selectivity. The below chromatogram is a five injection overlay with a resolution value of 1.5. Peaks: 1. Impurity 2. Phytonadione (E isomer) 3. Phytonadione (Z isomer) Method Conditions Column: Cogent UDC-Cholesterol™, 4μm, […]

Warfarin in a Tablet Analyzed with HPLC – AppNote

Excellent Peak Shape with an Easy to Run Method This Method is easy to prepare, use and reproduce. Note the Excellent Peak Shape for this Compound. This Methodology will give a long Column life and many Reproducible Runs. Peak: Warfarin Method Conditions Column: Cogent Bidentate C18™, 4μm, 100Å Catalog No.: 40018-75P Dimensions: 4.6 x 75mm […]

β-Carotene Capsule Analyzed with HPLC – AppNote

Separation from Matrix Peaks Beta-carotene may be taken as a dietary supplement in capsule form. In this case, a wide variety of Matrix Peaks were observed in the chromatographic data. It is possible that some of these peaks are various isomers of all-trans ß-carotene or other similar carotenes. In any case, resolution was obtained from […]

Category: AppNotes Reversed Phase Methods

Valsartan and Hydrochlorothiazide Analyzed with HPLC – AppNote

Vanillic Acid and 6′-O-Feruloylsucrose Analyzed with LCMS – AppNote

Vitamin K Isomers Analyzed with HPLC – AppNote

Warfarin in a Tablet Analyzed with HPLC – AppNote

β-Carotene Capsule Analyzed with HPLC – AppNote

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