AppNotes Reversed Phase Methods – MicroSolv Technology Corporation

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10 Phenolic Acids Separated with LCMS – AppNote

Compounds Shown were Extracted from Commercial Rice. Commercial Rice Extract 3 was spiked with ten standards and analyzed. The Cogent Phenyl Hydride Column was an excellent choice to use for analysis of phenolic compounds in the sample with Reversed Phase HPLC. The retention and separation of all nine available standards was possible using the method […]

4’-Aminoacetophenone: Drug Metabolite – AppNote

4’-aminoacetophenone Attachments No 66 Drug Metabolite.pdf 0.2 Mb Download File

5 Phenolic Compounds Separated by LCMS – AppNote

Commercial Rice Extract A commercial rice extract was analyzed and peaks were assigned based on retention times and m/z values for the compounds of interest using the Cogent Phenyl Hydride Column . After method validation the developed procedure can be used to evaluate the quality of rice and to develop the best extraction procedure. Peaks: […]

A Cannabis Metabolite in Urine Matrix Analyzed With LCMS – AppNote

Retention, Detection and Quantitation of a Cannabis Metabolite from Urine Samples Using the SPE technique in “Sample Preparation” section of this AppNote, successful MS detection of a Cannabis Metabolite using the Cogent Phenyl Hydride™ HPLC Column is described. It is worth noting that the retention of this compound was achieved using Methanol rather than Acetonitrile […]

Acetaminophen & impurity – AppNote

Drug Substance by HPLC Attachments No 68 Acetaminophen.pdf 0.1 Mb Download File

Acetaminophen Impurities Method – AppNote

Acetaminophen and two of its major impurities were analyzed using the Cogent Bidentate C18 Column and a simple Mobile Phase. The Peak Shapes were very high. The Repeatability of the Results was extremely good (%RSD = 0.01). Peaks: 1. 4-aminophenol 1.072 minutes 2. Acetaminophen 4.668 minutes 3. 4-nitrophenol 7.588 minutes t0: 0.9 minutes Method Conditions: […]

Advantage of Using Cogent TYPE-C Columns for Drugs of Abuse Analysis – AppNote

Analysis of drugs of abuse encompasses a wide variety of compounds, including controlled substances, prescription drugs, and even over-the-counter formulations. These drugs can be problematic to analyze due to several problems. First, many have similar structures (e.g. morphine and hydromorphone), making adequate selectivity difficult to obtain in a chromatographic separation. Second, a good number of […]

Advantages of Cogent HPLC Columns for Analyzing Amazonian Bark Extract – AppNote

The Amazon rainforest is the most biodiverse region in the world. Over one quarter of natural medicines have been found in rainforests, and new species are constantly being discovered. Therefore, there is a need for analytical study of the indigenous flora and fauna in developing new pharmaceuticals. In some cases, natives of the region have […]

Aegeline in Protein Sport Mix – AppNote

LC-MS analysis of harmful substance in food supplement The data demonstrates the use of the Cogent Bidentate C18 2.o™ Column for analysis of dietary supplement samples. Isolation of the Aegeline peak is readily obtained with an extracted ion chromatogram (EIC) in the positive ion mode. Chromatographically, the peak is well retained and highly symmetrical. The […]

Amlodipine, Valsartan, and Hydrochlorothiazide Tablet USP Assay – AppNote

This AppNote demonstrates the USP Method for Amlodipine, Valsartan, and Hydrochlorothiazide Tablets working with the Cogent RP C18. As shown in the Chromatograms, the Peak efficiency is superb and USP Peak tailing guidelines are easily met with the Column. This demonstrates a great alternative Column for easy plug and play with this USP Method. Method […]

Amoxicillin, USP Method – AppNote

Robust Analysis of Amoxicillin, Antibiotic by HPLC The USP assay method for Amoxicillin often yields poor peak shapes and low method reproducibility with L1 Columns based on ordinary silica. In contrast, the figures shown here illustrate the high degree of reproducibility obtained from use of the Cogent Bidentate C18 L1 Column, following the USP Assay […]

An Analysis of Local Anesthetic Dyclonine HCL by HPLC – AppNote

A Reproducible Method for Detection of Dyclonine Hydrochloride using Reversed Phase HPLC A rapid, sensitive, and reproducible method has been developed for detection of Dyclonine HCL. The data below, (overlay of 20 injections) illustrates how the compound can be adequately retained and detected using HPLC, with both excellent peak shape and run-to-run precision. Using a […]

Analgesic Drugs – AppNote

Excellent Peak Shapes Attachments No 107 Analgesic Drugs.pdf 0.2 Mb Download File

Analysis of Alpha and Beta-Acids in Hops – AppNote

Robust, precise method for beer samples Attachments No 245 Analysis of Alpha and Beta-Acids in Hops.pdf 0.2 Mb Download File

Analysis of Isobaric Opioids by LCMS – AppNote

Separation of Isobaric Drugs Morphine and Hydromorphone are Isobaric Drugs (the same m/z value) that can be separated using the Cogent Bidentate C18 2.o™ Column. Multiple samples (n=5) were prepared and analyzed. The results showed great reproducibility (RSDs < 3%). The detection limits were 50 to 500 times lower than that required by immunoassays. Peaks: […]

Analysis of MDMA in Plasma Samples by LCMS – AppNote

MDMA – Analyzed by HPLC with MS Under the described conditions, MDMA was retained and eluted as a symmetrical peak. The sensitivity of the method is very good and comparable to that reported with GC-MS detection [1]. Matrix effects were of minor extent and reproducible and hence should not compromise quantification. The method can be […]

Aspirin & Salicylic Acid – AppNote

Excellent Peak Shape For a Very Difficult Compound Attachments No 86 Aspirin & Salicylic Acid.pdf 0.1 Mb Download File

Atorvastatin forced degradation – AppNote

Separation of API from its lactone degradation product Attachments No 126 Forced Degradation of Atorvastatin (LC-MS).pdf 0.1 Mb Download File

Atorvastatin forced degradation of by HPLC – AppNote

Separation of API from its main degradation product Attachments No 125 Forced Degradation of Atorvastatin.pdf 0.1 Mb Download File

Atorvastatin HPLC Method Transfer to UHPLC – AppNote

Transfer to “near-UHPLC” 2.2um Column, for the API Atorvastatin This Application Note demonstrates how data obtained on a 4μm Cogent Bidentate C18 Column (Figure B) can be adapted for a 2.2μm Column (Figure A). The retention times of both analytes are very comparable in each figure. This method produces excellent Separation of the API and […]

Atropine determination using a simple gradient – AppNote

No ion pair reagent necessary and still very precise Attachments No 117 Precise Determination of Atropine using a simple gradient.pdf 0.1 Mb Download File

Barbiturates in Urine Analyzed by LCMS – AppNote

Phenobarbital-D5, Phenobarbital, Butalbital-D5, Butalbital, Pentobarbital-D5, Pentobarbital, Seconbarbital Attachments No 270 Barbiturates in Urine.pdf 0.3 Mb Download File

Benzonatate Excipients & Degradants – AppNote

Difficult to Separate due to Secondary Amine Attachments No 80 Benzonatate Excipients & Degradants.pdf 0.1 Mb Download File

Bi-Est® Method Transfer – AppNote

4μm to Near-UHPLC – Separation of Hormones: Estriol, Estradiol, and Progesterone Separation of three components of a hormone replacement formulation is demonstrated in this Application Note using the Cogent Bidentate C18 2.o™ Column. The two figures demonstrate how comparable retention can be obtained for both the near-UHPLC column as well as the standard 4μm Column, […]

Bisacodyl Tablet – AppNote

Separation of API from matrix peaks in real formulation Attachments No 227 Bisacodyl Tablet.pdf 0.3 Mb Download File

Bisphenol A, BPA an HPLC Method – AppNote

Toxic Substance Found in Consumer Products Analyzed by HPLC Bisphenol A (BPA) is a challenging compound for analysis by HPLC. Monitoring of this environmental and health toxicant is a necessary process for surveillance as well as risk assessment. As shown in the chromatograms below (5 overlaid), a Cogent Bidentate C8 Column produces Peak shapes that […]

Bisphenol A, S, & F Separated by HPLC – AppNote

Separation of Epoxy Resins Found in Consumer Products & Food Many consumer products are using BPA alternatives in recent years due to health concerns regarding toxicity of these compounds. An analytical HPLC method is shown in this AppNote to distinguish amongst these various Bisphenol compounds potentially in chemicals and foods. Excellent resolution was obtained for […]

Caffeine Content in Coffee Analyzed by HPLC – AppNote

Espresso, Regular, and De-Caffeinated Coffee Caffeine Content Caffeine is separated from other matrix components in three common Coffee beverages in this AppNote. A shot of Espresso (Figure A) had the highest content, followed by regular drip Coffee (Figure B) as expected. In De-Caffeinated Coffee, Caffeine is extracted from the coffee beans but even after numerous […]

Cannabis Metabolite in Urine Matrix – AppNote

Cannabis Metabolite in Urine Matrix is well retained on a Cogent Phenyl Hydride HPLC Column. The extraction procedure using an SPE technique allows for successful detection of this Cannabis metabolite with the Cogent Phenyl Hydride Column. It is worth noting that the retention of this compound was achieved using Methanol rather than Acetonitrile as the […]

Capsaicin in Topical Solution – AppNote

Separation of capsaicinoids Attachments No 222 Capsaicin in Topical Solution.pdf 0.3 Mb Download File

Carisoprodol & Meprobamate – AppNote

Analysis of closely related compounds Attachments No 124 Carisoprodol and Meprobamate.pdf 0.2 Mb Download File

Cefpodoxime Proxetil – AppNote

USP Assay Method Attachments No 149 Cefpodoxime Proxetil.pdf 0.3 Mb Download File

Cefprozil E & Z Isomers – AppNote

LC-MS Compatible Method Attachments No 158 Cefprozil E and Z Isomers.pdf 0.3 Mb Download File

Celecoxib – AppNote

Impurities Method with Improved Peak Shape Attachments No 148 Celecoxib.pdf 0.3 Mb Download File

Chlorpheniramine Maleate Organic Impurities Analyzed by HPLC – AppNote

Separation demonstrated in real commercial formulation extracts Use of the Cogent Bidentate C8™ Column allows for high resolution baseline separation of six specified impurities of Chlorpheniramine Maleate (Fig. A). Fig. B shows how the method can be applied to a real-world formulation, spiked with the N-oxide impurity to demonstrate resolution from the API peak. Currently, […]

Clindamycin Antiobiotic Tablet – AppNote

Easy separation from excipients Attachments No 71 Easy Separation from Excipients.pdf 0.2 Mb Download File

Codeine and Hydrocodone in Blood Analyzed by LCMS – AppNote

LC-MS Separation of isobaric Drugs in Urine Codeine and Hydrocodone which are isobaric drugs (i.e. the same m/z value) were separated using this AppNote. Multiple samples (n=5) were prepared and analyzed. The results showed excellent reproducibility (RSDs < 2.5%). The procedure in this AppNote, after validation, can be used in forensic toxicological laboratories to determine […]

Comparison of 4 μm vs. 2.o Columns in Analysis of Drospirenone & Ethinyl Estradiol Tablet – AppNote

Easy Method Transfer from 4µm to 2.o™ Columns. The Bidentate C18™ is an excellent choice for reversed phase applications. In addition to the standard 4µm material, a near-UHPLC 2.o™ phase is also available. These 2.2µm Columns provide inherently superior efficiency than a larger particle size. Because the same C18 ligand is used in both phases, […]

Coricidin HBP Day® Tablet – AppNote

Formulation of Guaifenesin and Dextromethorphan HBr Attachments No 206 Coricidin HBP Day Tablet.pdf 0.3 Mb Download File

Coricidin HBP Night® Tablet – AppNote

Acetaminophen, Dextromethorphan, and Chlorpheniramine This method demonstrates the potential of the Cogent Phenyl Hydride 2.o™ Column for analysis of amine-containing analytes in a real formulation. Chlorpheniramine and Dextromethorphan can often be problematic in terms of peak tailing due to the tertiary amines, but this column and method produces excellent peak shapes for both compounds in this […]

Cough syrup ingredient analysis by HPLC – AppNote

Separation of Antitussives, Analgesics, Decongestants, and Preservatives Attachments No 174 Cough Syrup Ingredients.pdf 0.4 Mb Download File

Cyanidin-3-O-Glucoside Analysis in Fruit- AppNote

Analysis in Fruit and Vegetable Extracts In this analysis of proprietary fruit & vegetable extracts, the presence of Cyanidin-3-O-Glucoside was confirmed in three out of four extracts. The peaks were symmetrical and retained beyond the dead volume. The method after validation can be used to quality control of commercial fruit extracts as well in studies […]

Cyclobenzaprine Hydrochloride USP Assay – AppNote

This method demonstrates the USP Cyclobenzaprine Hydrochloride Extended-Release Capsules Assay. As shown in the Chromatograms, the peak efficiency is superb and USP peak tailing guidelines are easily met with the Cogent RP C18 5μm column. This demonstrates a great alternative column for easy plug and play and this UPS Assay Method. Method Conditions: Column: Cogent […]

Cytochrome c – AppNote

From Horse and Bovine Heart Attachments No 92 Cytochrome c.pdf 0.1 Mb Download File

Dehydroepiandrosterone (DHEA) Capsule Separation by HPLC – AppNote

Excellent separation from matrix excipients Pharmaceutical formulations may contain a number of excipients that can potentially interfere with a chromatographic analysis. However, using the Cogent Bidentate C8 Column, the DHEA peak is well-resolved from the others, the latter of which all elute as a large peak in the solvent front portion of the run. The […]

Determination of an Antibiotic – AppNote

Using Carboxylic Acid Phase Attachments No 30 Determination of an Antibiotic.pdf 0.1 Mb Download File

Determination of Phenolic Compound by LCMS – AppNote

Compounds in Olive Leaves Extract A commercial olive extract was analyzed using the Cogent Phenyl Hydride Column. Only one oleuropein peak was detected and it was symmetrical and well retained. The results were reproducible (%RSD = 0.2 for retention times). According to the literature [1] the extract from olive leaves should contain additional compounds. To […]