Acetaminophen Impurities Method – AppNote

Acetaminophen and two of its major impurities were analyzed using the Cogent Bidentate C18 Column and a simple Mobile Phase. The Peak Shapes were very high. The Repeatability of the Results was extremely good (%RSD = 0.01). Peaks: 1. 4-aminophenol 1.072 minutes 2. Acetaminophen 4.668 minutes 3. 4-nitrophenol 7.588 minutes t0: 0.9 minutes Method Conditions: […]

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Advantage of Using Cogent TYPE-C Columns for Drugs of Abuse Analysis – AppNote

Analysis of drugs of abuse encompasses a wide variety of compounds, including controlled substances, prescription drugs, and even over-the-counter formulations. These drugs can be problematic to analyze due to several problems. First, many have similar structures (e.g. morphine and hydromorphone), making adequate selectivity difficult to obtain in a chromatographic separation. Second, a good number of […]

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Aegeline in Protein Sport Mix – AppNote

LC-MS analysis of harmful substance in food supplement The data demonstrates the use of the Cogent Bidentate C18 2.o™ Column for analysis of dietary supplement samples. Isolation of the Aegeline peak is readily obtained with an extracted ion chromatogram (EIC) in the positive ion mode. Chromatographically, the peak is well retained and highly symmetrical. The […]

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Amoxicillin, USP Method – AppNote

Robust Analysis of Amoxicillin, Antibiotic by HPLC The USP assay method for Amoxicillin often yields poor peak shapes and low method reproducibility with L1 Columns based on ordinary silica. In contrast, the figures shown here illustrate the high degree of reproducibility obtained from use of the Cogent Bidentate C18 L1 Column, following the USP Assay […]

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Analysis of Isobaric Opioids by LCMS – AppNote

Separation of Isobaric Drugs Morphine and Hydromorphone are Isobaric Drugs (the same m/z value) that can be separated using the Cogent Bidentate C18 2.o™ Column. Multiple samples (n=5) were prepared and analyzed. The results showed great reproducibility (RSDs < 3%). The detection limits were 50 to 500 times lower than that required by immunoassays. Peaks: […]

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Atorvastatin HPLC Method Transfer to UHPLC – AppNote

Transfer to “near-UHPLC” 2.2um Column, for the API Atorvastatin This Application Note demonstrates how data obtained on a 4μm Cogent Bidentate C18 Column (Figure B) can be adapted for a 2.2μm Column (Figure A). The retention times of both analytes are very comparable in each figure. This method produces excellent Separation of the API and […]

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Avoid Sample Cleanup for Food and Beverages – AppNote

3 Methods to Avoid Sample Cleanup with Histamine, Limonin & Folic Acid Food and beverage analysis is complicated by the complex nature of the sample. In many cases, extensive sample cleanup techniques such as Solid Phase Extraction (SPE) may be required to avoid problems associated with the sample matrix. In this Application Note, three strategies […]

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Bi-Est® Method Transfer – AppNote

4μm to Near-UHPLC – Separation of Hormones: Estriol, Estradiol, and Progesterone Separation of three components of a hormone replacement formulation is demonstrated in this Application Note using the Cogent Bidentate C18 2.o™ Column. The two figures demonstrate how comparable retention can be obtained for both the near-UHPLC column as well as the standard 4μm Column, […]

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Bisphenol A, S, & F Separated by HPLC – AppNote

Separation of Epoxy Resins Found in Consumer Products & Food Many consumer products are using BPA alternatives in recent years due to health concerns regarding toxicity of these compounds.  An analytical HPLC method is shown in this AppNote to distinguish amongst these various Bisphenol compounds potentially in chemicals and foods. Excellent resolution was obtained for […]

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BPA, BPF, and BPS in Water Transferred from Baby Bottles – AppNote

Ever since Bisphenol A (BPA) has become banned in baby bottle products in the U.S. due to its reported endocrine-disrupting effects, manufacturers have had to use alternative materials. Two such materials with properties similar to BPA are Bisphenol S (BPS) and Bisphenol F (BPF). The problem is that these compounds may in fact be just […]

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Caffeine Content in Coffee Analyzed by HPLC – AppNote

Espresso, Regular, and De-Caffeinated Coffee Caffeine Content Caffeine is separated from other matrix components in three common Coffee beverages in this AppNote. A shot of Espresso (Figure A) had the highest content, followed by regular drip Coffee (Figure B) as expected. In De-Caffeinated Coffee, Caffeine is extracted from the coffee beans but even after numerous […]

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Codeine and Hydrocodone in Blood Analyzed by LCMS – AppNote

LC-MS Separation of isobaric Drugs in Urine Codeine and Hydrocodone which are isobaric drugs (i.e. the same m/z value) were separated using this AppNote. Multiple samples (n=5) were prepared and analyzed. The results showed excellent reproducibility (RSDs < 2.5%). The procedure in this AppNote, after validation, can be used in forensic toxicological laboratories to determine […]

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Dantrolene Sodium Analyzed by HPLC with UV – AppNote

Dantrolene Sodium has several amine groups that can interact with lone-silanols causing problematic peak tailing when analyzed with conventional HPLC columns. By utilizing a Bidentate C18™ Column with its Silica Hydride™ surface, challenging compounds like Dantrolene can be readily retained with symmetrical peak shape. Peak:  Dantrolene Sodium Method Conditions: Column: Cogent Bidentate C18™, 4μm, 100Å […]

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Glycosidic Flavonoids Analyzed by HPLC – AppNote

Separation of Diosmin, Eriocitrin, and Naringin In this method, three Glycosidic flavonoid standards are separated with good resolution. The Cogent Bidentate C18 2.o™ Column produces high efficiency peaks with reproducible retention (see figure overlay). Separation is also observed for a small impurity peak, eluting between peaks 1 and 2. This separation of standards could be […]

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Hesperidin Analyzed by HPLC – AppNote

LC-MS compatible method This method for analysis of Hesperidin produces an excellent analyte peak and shows separation from what appears to be two impurity peaks. The gradient method is LC-MS compatible and can be applied to many types of Hesperidin-containing samples such as citrus fruit extracts. The overlay of five runs shown in the figure […]

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Hormone Replacement HPLC Methods – AppNote

Chromatographic Separation between Various Components in Hormone Replacement Formulations. Often, these formulations have multiple active ingredients present, therefore HPLC is an appropriate technique in obtaining the necessary specificity for a quantitative assay. The Cogent Bidentate C8 and C18™ Columns retain the hormones by conventional Reversed Phase HPLC, while the UDC-Cholesterol™ can also differentiate among the […]

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Indomethacin Capsule Analyzed by HPLC – AppNote

Simple Assay Method  This method for analysis of Indomethacin capsules is easy to perform and uses an LC-MS compatible Mobile Phase. The API peak that was obtained shows excellent efficiency and the data is very reproducible, as illustrated by the overlay of five runs shown in the figure. Peak: Indomethacin Method Conditions Column: Cogent Bidentate […]

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Lansoprazole Capsule Analyzed by HPLC – AppNote

Separation from matrix peaks Lansoprazole is separated from several Matrix Components in this simple assay method. The Mobile Phase is LC-MS compatible so the method could be applied to more complex samples such as plasma. Five runs are shown in the figure to illustrate the repeatability of the data. Peak:Lansoprazole Method Conditions Column: Cogent Bidentate […]

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Limonin in Orange Juice Analyzed by LC-MS – AppNote

Separation from Matrix Peaks with Reproducible Results Analysis of Limonin in Spiked Orange Juice was performed using a simple isocratic method, the Cogent Bidentate C18 Column, and MS detection. Analysis at 471.2 m/z allowed for detection of only the Limonin peak without interference of the juice matrix. When the Filtered, Unspiked Orange Juice was injected, […]

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