Simple Isocratic LCMS Compatible Assay Method
The USP Assay Method for Fenofibric Acid uses a Phosphate buffer which is incompatible with MS detection. In this isocratic Method, only Formic Acid is needed in the Mobile Phase. The Peak Efficiency and Symmetry are excellent and the run-to-run precision is reliable and robust, as the 10 Chromatograms shown below indicate.
Column: Cogent Bidentate C18™, 4µm, 100Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75mm
Mobile Phase: 50% DI Water / 50% Acetonitrile / 0.1% Formic Acid (v/v)
Injection vol.: 1µL
Flow rate: 1.0mL / minute
Detection: UV @ 285nm
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Note: Fenofibric Acid is used to moderate Cholesterol levels for patients at risk for cardiovascular diseases. It lowers the amount of Triglycerides and low-density Lipoprotein (LDL) Cholesterol, as well as increases the amount of high-density Lipoprotein (HDL) Cholesterol. It is often used in combination with Statins.
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