1,4-Benzodiazepines in Urine Analyzed using RP and ANP – AppNote

The analyses of 1,4-Benzodiazepines using the Cogent Diol Column from Urine Samples after Solid Phase Extraction using two types of Retention Modes are shown in this Note. Four Compounds were well retained and separated in both HPLC Modes shown in Figures A and B; however, the Retention Order was changed depending on the Gradient used. It […]

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1,4-Benzodiazepines in Urine by ANP – AppNote

1,4-Benzodiazepines from Urine Samples were analyzed using a Cogent Diamond Hydride 2.o HPLC Column successfully after Solid Phase Extraction (SPE). Four available Compounds (shown below) were well retained and separated. The procedure could be used for determination of this class of compounds in urine samples and other body fluids. Peaks: 1. Temazepam 301.0739 m/z [M+H]+ […]

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10 Phenolic Acids Separated with LCMS – AppNote

Compounds Shown were Extracted from Commercial Rice. Commercial Rice Extract 3 was spiked with ten standards and analyzed. The Cogent Phenyl Hydride Column was an excellent choice to use for analysis of phenolic compounds in the sample with Reversed Phase HPLC. The retention and separation of all nine available standards was possible using the method […]

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3-Hydroxy-3-Methylglutaric acid, HMG in Urine by HPLC – AppNote

Important Biomarker for Diabetes and HMG Disorder is well retained by HPLC. A selective, specific, and sensitive HPLC method with LCMS Analysis has been developed for the determination of 3-hydroxy-3-methylglutaric acid (a.k.a. Meglutol) in urine samples. The method can be also used in the analysis of plasma samples after precipitation of plasma proteins with Acetonitrile. […]

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3,3′-Diaminobenzidine by LCMS – AppNote

Retention and Separation is easy with the Cogent Diamond Hydride Column with the Method below. Normally this compound is very hard to get good peak shapes in Reversed Phase. Peak: 3,3′-Diaminobenzidine 215.1291 m/z (M+H)+ Method Conditions: Column: Cogent Diamond Hydride™, 4µm, 100Å Catalog No.: 70000-15P-2 Dimensions: 2.1 x 150 mm Solvents: A: 50% DI Water/ […]

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5 Phenolic Compounds Separated by LCMS – AppNote

Commercial Rice Extract A commercial rice extract was analyzed and peaks were assigned based on retention times and m/z values for the compounds of interest using the Cogent Phenyl Hydride Column . After method validation the developed procedure can be used to evaluate the quality of rice and to develop the best extraction procedure. Peaks: […]

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A Cannabis Metabolite in Urine Matrix Analyzed With LCMS – AppNote

Retention, Detection and Quantitation of a Cannabis Metabolite from Urine Samples  Using the SPE technique in “Sample Preparation” section of this AppNote, successful MS detection of a Cannabis Metabolite using the Cogent Phenyl Hydride™ HPLC Column is described. It is worth noting that the retention of this compound was achieved using Methanol rather than Acetonitrile […]

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Acetaminophen Impurities Method – AppNote

Acetaminophen and two of its major impurities were analyzed using the Cogent Bidentate C18 Column and a simple Mobile Phase. The Peak Shapes were very high. The Repeatability of the Results was extremely good (%RSD = 0.01). Peaks: 1. 4-aminophenol 1.072 minutes 2. Acetaminophen 4.668 minutes 3. 4-nitrophenol 7.588 minutes t0: 0.9 minutes Method Conditions: […]

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Acetone as the Organic Mobile Phase Component – AppNote

“Green” substitute for separation of amino acids Caution: Acetone and Amines can react When working with Acetone as an HPLC solvent it is important to note that primary amines could react with Acetone to form imines. However, to achieve this reaction the mobile phase would most likely need to be free of water and or […]

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Advantage of Using Cogent TYPE-C Columns for Drugs of Abuse Analysis – AppNote

Analysis of drugs of abuse encompasses a wide variety of compounds, including controlled substances, prescription drugs, and even over-the-counter formulations. These drugs can be problematic to analyze due to several problems. First, many have similar structures (e.g. morphine and hydromorphone), making adequate selectivity difficult to obtain in a chromatographic separation. Second, a good number of […]

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Aegeline in Protein Sport Mix – AppNote

LC-MS analysis of harmful substance in food supplement The data demonstrates the use of the Cogent Bidentate C18 2.o™ Column for analysis of dietary supplement samples. Isolation of the Aegeline peak is readily obtained with an extracted ion chromatogram (EIC) in the positive ion mode. Chromatographically, the peak is well retained and highly symmetrical. The […]

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Alprazolam (Xanax®) HPLC Method Transferred – AppNote

Benefit of 2.2um Column Analyzing Alprazolam by HPLC In this AppNote, we show that a transfer from a 4µm to 2.2µm Column, higher efficiency is observed with the smaller particle size Column. This can be very useful for analyses requiring detection at low concentrations. The Peak shape is very sharp using the 2.2um Column (Figure. […]

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Amikacin & Tobramycin – AppNote

Highly Polar Antibiotic Separation These two Antibiotic agents present a considerable challenge to chromatographers. As highly hydrophilic compounds, they are generally poorly retained by conventional Reversed Phase methods that rely on an analytes hydrophobicity. In addition, they exhibit very low UV absorption and therefore more sophisticated detection methods are often required. In this application, an […]

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Amiloride and Triamterene in Urine – AppNote

LCMS Method for Spiked Urine Sample This application note describes the separation of two important  drugs for treatment of hypertension and congestive heart failure. Both compounds have numerous amine groups, which can contribute to Peak tailing in some instances. However, the Peak shapes observed here are both symmetrical and very efficient. This is accomplished using […]

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Amlodipine, Valsartan, and Hydrochlorothiazide Tablet USP Assay – AppNote

This AppNote demonstrates the USP Method for Amlodipine, Valsartan, and Hydrochlorothiazide Tablets working with the Cogent RP C18. As shown in the Chromatograms, the Peak efficiency is superb and USP Peak tailing guidelines are easily met with the Column. This demonstrates a great alternative Column for easy plug and play with this USP Method. Method […]

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Amoxicillin, USP Method – AppNote

Robust Analysis of Amoxicillin, Antibiotic by HPLC The USP assay method for Amoxicillin often yields poor peak shapes and low method reproducibility with L1 Columns based on ordinary silica. In contrast, the figures shown here illustrate the high degree of reproducibility obtained from use of the Cogent Bidentate C18 L1 Column, following the USP Assay […]

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An Analysis of Local Anesthetic Dyclonine HCL by HPLC – AppNote

A Reproducible Method for Detection of Dyclonine Hydrochloride using Reversed Phase HPLC A rapid, sensitive, and reproducible method has been developed for detection of Dyclonine HCL. The data below, (overlay of 20 injections) illustrates how the compound can be adequately retained and detected using HPLC, with both excellent peak shape and run-to-run precision. Using a […]

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Analysis of Isobaric Opioids by LCMS – AppNote

Separation of Isobaric Drugs Morphine and Hydromorphone are Isobaric Drugs (the same m/z value) that can be separated using the Cogent Bidentate C18 2.o™ Column. Multiple samples (n=5) were prepared and analyzed. The results showed great reproducibility (RSDs < 3%). The detection limits were 50 to 500 times lower than that required by immunoassays. Peaks: […]

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Analysis of Litchi Seed Extracts – AppNote

Hypoglycin A and Methylenecyclopropylglycine are two compounds found in the extract of Litchi Seeds that studies have shown to be toxic. Methylenecyclopropylglycine inhibits the synthesis of fatty acids from Glucose, and therefore undernourished children are more at risk since their Glucose reserves are low. This study used LC-MS with a Cogent Diamond Hydride™ Column to […]

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Analysis of MDMA in Plasma Samples by LCMS – AppNote

MDMA – Analyzed by HPLC with MS Under the described conditions, MDMA was retained and eluted as a symmetrical peak. The sensitivity of the method is very good and  comparable to that reported with GC-MS detection [1]. Matrix effects were of minor extent and reproducible and hence should not compromise quantification. The method can be […]

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Analysis of metabolites such as glucose, pyruvate, taurocholic acid, gamma glutamylcysteine – AppNote

What suggestion can you offer for Analysis of Metabolites such as glucose, pyruvate, taurocholic acid, gamma glutamylcysteine, glycochenodeoxycholate, glycerol-3-phosphate, spermidine, taurine, and S-Adenosylmethionine? Suggestion: We would recommend the Cogent Diamond Hydride™ HPLC Column for this Analysis. Many of these compounds are quite polar and would be very difficult to separate by Conventional Reversed Phase Methods. […]

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Analysis of Thiopurines – AppNote

Separation using LCMS Gradient Method The Cogent Diamond Hydride Column with gradient elution was used for analysis of Thiopurines. Figure A shows the Peak of 6-TG, which also can be analyzed using Isocratic conditions. Good retention and symmetrical peak shape were obtained under the analysis conditions. Figure B represents two separated Thiopurines (Mono and Di- […]

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Analyzing Bisphenol A in Carbonless Paper – AppNote

Bisphenol A (BPA) is a synthetic compound used in a variety of consumer products, such as CDs, water bottles, and water pipe linings. Despite having many uses in these products, there has been some concern in recent years that exposure to BPA may have adverse health implications. BPA is believed to act as an endocrine […]

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Antibiotic Keflex Analysis by HPLC – AppNote

Analysis of Polar Antibiotic Main Active Ingredient: Cephalexin Monohydrate Cephalexin Monohydrate, commercially known as Keflex, is a semi synthetic Cephalosporin antibacterial drug, analysis of which is of great interest to scientists from many research fields. There is a need for a rapid, efficient and inexpensive analytical method to be applied for analysis of formulations during […]

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Application Notes – AppNotes Listed by Compound

This list of links to Knowledge Base Articles on this Site, showcase Methods of Separation for each of the Compounds below and the Cogent Column used.  Not all of our Application Notes are listed below and to find other Applications, call our Customer Service Department at 1-732-380-8912 between 9-5 EST or use our Click To […]

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Atorvastatin HPLC Method Transfer to UHPLC – AppNote

Transfer to “near-UHPLC” 2.2um Column, for the API Atorvastatin This Application Note demonstrates how data obtained on a 4μm Cogent Bidentate C18 Column (Figure B) can be adapted for a 2.2μm Column (Figure A). The retention times of both analytes are very comparable in each figure. This method produces excellent Separation of the API and […]

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ATP, UDP-Glucose UDP-Galactose Separation from Red Blood Cells – AppNote

Separation of Nucleotides  In this application, we present an easy-to-use protocol for sensitive detection and quantification of nucleotides in red blood cell extracts.  Retention times were very reproducible with %RSD around 0.4. The desired separation between UDP-Glu and UDP-Gal was achieved, all other nucleotides eluted later and did not interfere with the analysis. ATP represents […]

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