AppNotes HPLC – MicroSolv Technology Corporation

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1,4-Benzodiazepines in Urine Analyzed using RP and ANP – AppNote

The analyses of 1,4-Benzodiazepines using the Cogent Diol Column from Urine Samples after Solid Phase Extraction using two types of Retention Modes are shown in this Note. Four Compounds were well retained and separated in both HPLC Modes shown in Figures A and B; however, the Retention Order was changed depending on the Gradient used. It […]

1,4-Benzodiazepines in Urine by ANP – AppNote

1,4-Benzodiazepines from Urine Samples were analyzed using a Cogent Diamond Hydride 2.o HPLC Column successfully after Solid Phase Extraction (SPE). Four available Compounds (shown below) were well retained and separated. The procedure could be used for determination of this class of compounds in urine samples and other body fluids. Peaks: 1. Temazepam 301.0739 m/z [M+H]+ […]

10 Phenolic Acids Separated with LCMS – AppNote

Compounds Shown were Extracted from Commercial Rice. Commercial Rice Extract 3 was spiked with ten standards and analyzed. The Cogent Phenyl Hydride Column was an excellent choice to use for analysis of phenolic compounds in the sample with Reversed Phase HPLC. The retention and separation of all nine available standards was possible using the method […]

3-Hydroxy-3-Methylglutaric acid, HMG in Urine by HPLC – AppNote

Important Biomarker for Diabetes and HMG Disorder is well retained by HPLC. A selective, specific, and sensitive HPLC method with LCMS Analysis has been developed for the determination of 3-hydroxy-3-methylglutaric acid (a.k.a. Meglutol) in urine samples. The method can be also used in the analysis of plasma samples after precipitation of plasma proteins with Acetonitrile. […]

3,3′-Diaminobenzidine by LCMS – AppNote

Retention and Separation is easy with the Cogent Diamond Hydride Column with the Method below. Normally this compound is very hard to get good peak shapes in Reversed Phase. Peak: 3,3′-Diaminobenzidine 215.1291 m/z (M+H)+ Method Conditions: Column: Cogent Diamond Hydride™, 4µm, 100Å Catalog No.: 70000-15P-2 Dimensions: 2.1 x 150 mm Solvents: A: 50% DI Water/ […]

4’-Aminoacetophenone: Drug Metabolite – AppNote

4’-aminoacetophenone Attachments No 66 Drug Metabolite.pdf 0.2 Mb Download File

5 Phenolic Compounds Separated by LCMS – AppNote

Commercial Rice Extract A commercial rice extract was analyzed and peaks were assigned based on retention times and m/z values for the compounds of interest using the Cogent Phenyl Hydride Column . After method validation the developed procedure can be used to evaluate the quality of rice and to develop the best extraction procedure. Peaks: […]

A Cannabis Metabolite in Urine Matrix Analyzed With LCMS – AppNote

Retention, Detection and Quantitation of a Cannabis Metabolite from Urine Samples Using the SPE technique in “Sample Preparation” section of this AppNote, successful MS detection of a Cannabis Metabolite using the Cogent Phenyl Hydride™ HPLC Column is described. It is worth noting that the retention of this compound was achieved using Methanol rather than Acetonitrile […]

Acetaminophen & impurity – AppNote

Drug Substance by HPLC Attachments No 68 Acetaminophen.pdf 0.1 Mb Download File

Acetaminophen Impurities Method – AppNote

Acetaminophen and two of its major impurities were analyzed using the Cogent Bidentate C18 Column and a simple Mobile Phase. The Peak Shapes were very high. The Repeatability of the Results was extremely good (%RSD = 0.01). Peaks: 1. 4-aminophenol 1.072 minutes 2. Acetaminophen 4.668 minutes 3. 4-nitrophenol 7.588 minutes t0: 0.9 minutes Method Conditions: […]

Acetone as the Organic Mobile Phase Component – AppNote

“Green” substitute for separation of amino acids Caution: Acetone and Amines can react When working with Acetone as an HPLC solvent it is important to note that primary amines could react with Acetone to form imines. However, to achieve this reaction the mobile phase would most likely need to be free of water and or […]

Acetyl-L-Carnitine (ALC) in Plasma – AppNote

Excellent LC-MS method in spiked plasma sample For more detailed application, click here. Attachments No 240 Acetyl-L-Carnitine in Plasma.pdf 0.3 Mb Download File

Acrylamide HPLC analysis method – AppNote

Easy & Precise Retention Attachments No 199 Acrylamide.pdf 0.3 Mb Download File

Advantage of Organic Normal Phase on C18 – AppNote

“Prep up” in NP and “Check Purity” in RP on the Same Analytical Column Attachments No 15 Advantage of Organic Normal Phase on C18.pdf 0.2 Mb Download File

Advantage of Using Cogent TYPE-C Columns for Drugs of Abuse Analysis – AppNote

Analysis of drugs of abuse encompasses a wide variety of compounds, including controlled substances, prescription drugs, and even over-the-counter formulations. These drugs can be problematic to analyze due to several problems. First, many have similar structures (e.g. morphine and hydromorphone), making adequate selectivity difficult to obtain in a chromatographic separation. Second, a good number of […]

Advantages of Cogent HPLC Columns for Analyzing Amazonian Bark Extract – AppNote

The Amazon rainforest is the most biodiverse region in the world. Over one quarter of natural medicines have been found in rainforests, and new species are constantly being discovered. Therefore, there is a need for analytical study of the indigenous flora and fauna in developing new pharmaceuticals. In some cases, natives of the region have […]

Aegeline in Protein Sport Mix – AppNote

LC-MS analysis of harmful substance in food supplement The data demonstrates the use of the Cogent Bidentate C18 2.o™ Column for analysis of dietary supplement samples. Isolation of the Aegeline peak is readily obtained with an extracted ion chromatogram (EIC) in the positive ion mode. Chromatographically, the peak is well retained and highly symmetrical. The […]

Alprazolam (Xanax®) – AppNote

Robust Separation of API from USP Internal Standard Attachments No 183 Alprazolam Xanax.pdf 0.6 Mb Download File

Alprazolam (Xanax®) HPLC Method Transferred – AppNote

Benefit of 2.2um Column Analyzing Alprazolam by HPLC In this AppNote, we show that a transfer from a 4µm to 2.2µm Column, higher efficiency is observed with the smaller particle size Column. This can be very useful for analyses requiring detection at low concentrations. The Peak shape is very sharp using the 2.2um Column (Figure. […]

Amikacin & Tobramycin – AppNote

Highly Polar Antibiotic Separation These two Antibiotic agents present a considerable challenge to chromatographers. As highly hydrophilic compounds, they are generally poorly retained by conventional Reversed Phase methods that rely on an analytes hydrophobicity. In addition, they exhibit very low UV absorption and therefore more sophisticated detection methods are often required. In this application, an […]

Amiloride and Triamterene in Urine – AppNote

LCMS Method for Spiked Urine Sample This application note describes the separation of two important drugs for treatment of hypertension and congestive heart failure. Both compounds have numerous amine groups, which can contribute to Peak tailing in some instances. However, the Peak shapes observed here are both symmetrical and very efficient. This is accomplished using […]

Amino Acids, Non Derivatized – AppNote

From Synthetic Urine Attachments No 58 Non Derivatized Amino Acids.pdf 0.2 Mb Download File

Amlodipine, Valsartan, and Hydrochlorothiazide Tablet USP Assay – AppNote

This AppNote demonstrates the USP Method for Amlodipine, Valsartan, and Hydrochlorothiazide Tablets working with the Cogent RP C18. As shown in the Chromatograms, the Peak efficiency is superb and USP Peak tailing guidelines are easily met with the Column. This demonstrates a great alternative Column for easy plug and play with this USP Method. Method […]

Amoxicillin – AppNote

Orthogonal retention method to USP assay Attachments No 135 Amoxicillin.pdf 0.2 Mb Download File

Amoxicillin, USP Method – AppNote

Robust Analysis of Amoxicillin, Antibiotic by HPLC The USP assay method for Amoxicillin often yields poor peak shapes and low method reproducibility with L1 Columns based on ordinary silica. In contrast, the figures shown here illustrate the high degree of reproducibility obtained from use of the Cogent Bidentate C18 L1 Column, following the USP Assay […]

An Analysis of Local Anesthetic Dyclonine HCL by HPLC – AppNote

A Reproducible Method for Detection of Dyclonine Hydrochloride using Reversed Phase HPLC A rapid, sensitive, and reproducible method has been developed for detection of Dyclonine HCL. The data below, (overlay of 20 injections) illustrates how the compound can be adequately retained and detected using HPLC, with both excellent peak shape and run-to-run precision. Using a […]

Analgesic Drugs – AppNote

Excellent Peak Shapes Attachments No 107 Analgesic Drugs.pdf 0.2 Mb Download File

Analysis of Alpha and Beta-Acids in Hops – AppNote

Robust, precise method for beer samples Attachments No 245 Analysis of Alpha and Beta-Acids in Hops.pdf 0.2 Mb Download File

Analysis of Isobaric Opioids by LCMS – AppNote

Separation of Isobaric Drugs Morphine and Hydromorphone are Isobaric Drugs (the same m/z value) that can be separated using the Cogent Bidentate C18 2.o™ Column. Multiple samples (n=5) were prepared and analyzed. The results showed great reproducibility (RSDs < 3%). The detection limits were 50 to 500 times lower than that required by immunoassays. Peaks: […]

Analysis of Litchi Seed Extracts – AppNote

Hypoglycin A and Methylenecyclopropylglycine are two compounds found in the extract of Litchi Seeds that studies have shown to be toxic. Methylenecyclopropylglycine inhibits the synthesis of fatty acids from Glucose, and therefore undernourished children are more at risk since their Glucose reserves are low. This study used LC-MS with a Cogent Diamond Hydride™ Column to […]

Analysis of MDMA in Plasma Samples by LCMS – AppNote

MDMA – Analyzed by HPLC with MS Under the described conditions, MDMA was retained and eluted as a symmetrical peak. The sensitivity of the method is very good and comparable to that reported with GC-MS detection [1]. Matrix effects were of minor extent and reproducible and hence should not compromise quantification. The method can be […]

Analysis of metabolites such as glucose, pyruvate, taurocholic acid, gamma glutamylcysteine – AppNote

What suggestion can you offer for Analysis of Metabolites such as glucose, pyruvate, taurocholic acid, gamma glutamylcysteine, glycochenodeoxycholate, glycerol-3-phosphate, spermidine, taurine, and S-Adenosylmethionine? Suggestion: We would recommend the Cogent Diamond Hydride™ HPLC Column for this Analysis. Many of these compounds are quite polar and would be very difficult to separate by Conventional Reversed Phase Methods. […]

Analysis of Nucleotides by ANP – AppNote

ATP & AMP separated in Aqueous Normal Phase (ANP) Attachments No 95 Analysis of Nucleotides by ANP.pdf 0.1 Mb Download File

Analysis of Thiopurines – AppNote

Separation using LCMS Gradient Method The Cogent Diamond Hydride Column with gradient elution was used for analysis of Thiopurines. Figure A shows the Peak of 6-TG, which also can be analyzed using Isocratic conditions. Good retention and symmetrical peak shape were obtained under the analysis conditions. Figure B represents two separated Thiopurines (Mono and Di- […]

Analyte Ionization using L-tyrosine – AppNote

L-Tyrosine capsule Attachments No 179 LC-MS Tips for Promoting Analyte Ionization.pdf 0.4 Mb Download File

Analyzing Bisphenol A in Carbonless Paper – AppNote

Bisphenol A (BPA) is a synthetic compound used in a variety of consumer products, such as CDs, water bottles, and water pipe linings. Despite having many uses in these products, there has been some concern in recent years that exposure to BPA may have adverse health implications. BPA is believed to act as an endocrine […]

Anatoxin-A Method Development Strategy for a Polar Compound – AppNote

Using Anatoxin-a Attachments No 142 Method Development Strategy for a Polar Compound.pdf 0.3 Mb Download File

Anatoxin-a, ANTX-A – AppNote

Potent Neurotoxin Attachments No 141 Anatoxin-a ANTX-A.pdf 0.3 Mb Download File

Antibiotic Keflex Analysis by HPLC – AppNote

Analysis of Polar Antibiotic Main Active Ingredient: Cephalexin Monohydrate Cephalexin Monohydrate, commercially known as Keflex, is a semi synthetic Cephalosporin antibacterial drug, analysis of which is of great interest to scientists from many research fields. There is a need for a rapid, efficient and inexpensive analytical method to be applied for analysis of formulations during […]

Application Notes – AppNotes Listed by Compound

This list of links to Knowledge Base Articles on this Site, showcase Methods of Separation for each of the Compounds below and the Cogent Column used. Not all of our Application Notes are listed below and to find other Applications, call our Customer Service Department at 1-732-380-8912 between 9-5 EST or use our Click To […]

Arginine, Lysine & Histidine, Basic Amino Acids – AppNote

In Synthetic or Human Urine Attachments No 60 Basic Amino Acids.pdf 0.3 Mb Download File

Aspartic Acid & Arginine: Amino Acids – AppNote

Reduced Sample Prep and Handling Attachments No 63 Easy Amino Acid Method.pdf 0.2 Mb Download File

Aspirin & Salicylic Acid – AppNote

Excellent Peak Shape For a Very Difficult Compound Attachments No 86 Aspirin & Salicylic Acid.pdf 0.1 Mb Download File

Asymmetric Dimethylarginine ADMA – AppNote

LC-MS method in plasma samples Attachments No 169 Asymmetric Dimethylarginine ADMA.pdf 0.3 Mb Download File

Atenolol Tablet – AppNote

No Ion Pairings Needed Attachments No 250 Atenolol Tablet.pdf 0.4 Mb Download File

Atorvastatin forced degradation – AppNote

Separation of API from its lactone degradation product Attachments No 126 Forced Degradation of Atorvastatin (LC-MS).pdf 0.1 Mb Download File

Atorvastatin forced degradation of by HPLC – AppNote

Separation of API from its main degradation product Attachments No 125 Forced Degradation of Atorvastatin.pdf 0.1 Mb Download File

Atorvastatin HPLC Method Transfer to UHPLC – AppNote

Transfer to “near-UHPLC” 2.2um Column, for the API Atorvastatin This Application Note demonstrates how data obtained on a 4μm Cogent Bidentate C18 Column (Figure B) can be adapted for a 2.2μm Column (Figure A). The retention times of both analytes are very comparable in each figure. This method produces excellent Separation of the API and […]

ATP, UDP-Glucose UDP-Galactose Separation from Red Blood Cells – AppNote

Separation of Nucleotides In this application, we present an easy-to-use protocol for sensitive detection and quantification of nucleotides in red blood cell extracts. Retention times were very reproducible with %RSD around 0.4. The desired separation between UDP-Glu and UDP-Gal was achieved, all other nucleotides eluted later and did not interfere with the analysis. ATP represents […]

Atropine determination – AppNote

Rugged & fast without paired ion reagents Attachments No 119 Determination of Atropine.pdf 0.1 Mb Download File