Acrylamide HPLC Analyzed with HPLC – AppNote – MicroSolv Technology Corporation

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August 13, 2012
Bill Ciccone

AppNotes ANP/HILIC Methods / AppNotes Chemicals/Energy / AppNotes Cogent Diamond Hydride / AppNotes Library / AppNotes Listed by Discipline / ARTICLES, APPNOTES, FAQ

Easy & Precise Retention of a Very Polar Compound

Acrylamide can be difficult to retain with conventional Reversed Phase Methods due to its polar nature. With this Method however, Retention is readily achievable using a simple, Isocratic Mobile Phase. The overlay of two Chromatograms using two different lots of HPLC Columns in the Figure below illustrates the Reproducibility and Robustness of this Method.

Peak:
Acrylamide

Method Conditions
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75mm
Mobile Phase: Acetonitrile with 0.1% Formic Acid
Injection vol.: 1μL
Flow rate: 1.0mL / minute
Detection: UV @ 205nm
Sample Preparation: 0.1mg / mL Acrylamide in Mobile Phase as the Diluent.
t0: 1.0 minute

Note: Acrylamide is a monomer used to synthesize polyacrylamides. It was reported to be present in certain food products in 2002. This has been cause for concern as the monomer form is a known carcinogen and neurotoxin. As such, quantitation of this analyte is of importance in a variety of fields.

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