I am not observing any peaks for succinic acid or alpha-ketoglutarate with the Cogent Diamond Hydride column – Tips & Suggestions Read More →
I heard that there is not a good alternative to HILIC to achieve sufficient retention for some compounds – FAQ Read More →
Is TFA or formic acid better as an acidic mobile phase additive for Cogent TYPE-C columns – FAQ Read More →
Lower retention is observed when functional group in a compound is ionized – Tips & Suggestions Read More →
My LCMS source was getting white film on it and at the same time the background noise was very low so is my Diamond Hydride column bleeding packing material – FAQ Read More →
No need to use a high pH mobile phases for compounds with a high pKa – Tips & Suggestions Read More →
Polar and non polar peptides analyzed by LCMS with an Aqueous Normal Phase ANP method – Tips & Suggestions Read More →
Restoring the initial results with a method for analyzing AMP ADP ATP UDP and GPT in biological extracts – Tips & Suggestions Read More →
Retention and or peak shape for citric acid has changed compared to data from a previous HPLC run – Tips & Suggestions Read More →
Should I use methanol as the organic solvent in HPLC when using Aqueous Normal Phase ANP – FAQ Read More →
Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible – FAQ Read More →
Since Cogent TYPE-C Silica columns use a transition metal catalyst in production what is the presence of any trace quantity on the surface of the stationary phase- FAQ Read More →
The effect of ionic strength of the mobile phase additive for HPLC columns in Aqueous Normal Phase – Tips & Suggestions Read More →
Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source – Tips & Suggestions Read More →
Using two HPLC columns together in a tandem separation for polar and non polar retention – Tips & Suggestions Read More →
What are some notable examples of journal articles that feature Aqueous Normal Phase ANP methods in metabolomics studies – FAQ Read More →
What can cause changing retention times and a peak showing up in my blank with a Silica-C column – FAQ Read More →
What is the compatibility of Cogent TYPE-C Silica HPLC columns with UHPLC instruments – FAQ Read More →
What pressure should I expect with a Diamond Hydride 2.o HPLC column at flow rates from 0.2 to 2.0mL / min – FAQ Read More →
What will the pressure be with a mobile phase of 90% methanol and 10% DI water with a 4.6 x 250mm Cogent TYPE-C HPLC column – FAQ Read More →
Which column do you suggest for Cocaine & metabolite Ecognin separation – Tips & Suggestions Read More →
Why am I observing broad split peaks in Aqueous Normal Phase ANP when using acetonitrile and methanol diluents – FAQ Read More →
Why do I have lower than normal back pressure with Cogent Diamond Hydride HPLC columns – FAQ Read More →
Why do I see large intermittent pressure spikes in HPLC runs and can it be a defective column – FAQ Read More →