Before the First use of the Diamond Hydride Column:
1. Condition it with 50:50 MeOH/Water for 30 minutes.
Conditioning Steps – Depends on Your Samples:
1. When using urine, plasma, or other biological samples etc:
A. Conditioning the Column with 80% Acetonitrile/ 20% DI water/ 0.2% Acetic Acid (or 0.1% Formic Acid) is recommended for 20 minutes.
B. Cleaning with 50% MeOH/ 50% Water is recommended for every 10 Injections for 5 minutes.
2. Using Standard Samples:
A: Conditioning with 80% Acetonitrile/ 20% DI Water/ 0.2% Acetic Acid (or 0.1% Formic Acid) is recommended for 20 minutes.
B. Cleaning with 50% MeOH/ 50% Water is recommended for every 100 Injections or sooner if change in performance is noticed, for 5 minutes.
Storing the column:
1. For long term storage, 100% ACN is best. Rinse with 10 Column Volumes.
2. For moderate length of time, 90:10 Acetonitrile/Water with 0.1% Formic or Acetic Acid. Rinse with six Column Volumes.
Washing the Column to Remove Adsorbed Compounds:
The best method for washing a Diamond Hydride Column is to use 50:50 Methanol/Water with 0.1% Formic Acid as rinse Solvent. Wash for 60 minutes using a slower Flow Rate than for your regular runs. Return to Acetonitrile / Water.
If the performance can still be improved, wash your Column overnight using 50:50 Isopropyl Alcohol/Water with 0.1% Formic Acid at a Low Flow Rate.
If needed, uninstall the Column and reinstall in a Reverse Direction and wash it with 50:50 Methanol/Water with 0.1% Formic Acid for 60 minutes.
Never let the column dry out.
