Method development tip for maleic hydrazide – Tips & Suggestions

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ARTICLES, APPNOTES, FAQ (9187)
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October 1, 2012
Bill Ciccone

2.o™ HPLC Columns FAQ / ANP Aqueous Normal Phase Articles / ARTICLES, APPNOTES, FAQ / Cogent Diamond Hydride Information / Diamond Hydride FAQ / How To... Information / HPLC Column Information / HPLC Columns FAQ / HPLC Tips and Suggestions / Reversed Phase Columns FAQ / Trouble Shooting Suggestions / TYPE-C Column Information / TYPE-C in General FAQ

Maleic hydrazide has a pKa of 5.62 and a logP of -1.96. This logP value means it is very hydrophilic and will NOT retain well in Reversed Phase HPLC.

For this reason, we would suggest you try a method in Aqueous Normal Phase ANP using the Cogent Diamond Hydride™ column in order to retain it. The pKa of 5.62 suggests that a mobile phase additive of 0.1% formic acid would work best. And 0.1% TFA can also be investigated if detection is not done by MS.

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