Maleic Hydrazide has a pKa of 5.62 and a logP of -1.96. This logP value means it is very Hydrophilic and will not retain well in Reversed Phase HPLC.

For this reason, we would suggest you try the Cogent Diamond Hydride™ column in Aqueous Normal Phase (ANP) mode in order to analyze and retain it. The pKa of 5.62 suggests that a mobile phase additive of 0.1% Formic Acid would work best. And 0.1% TFA can also be investigated if detection is not done by MS.

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