AppNotes ANP/HILIC Methods – MicroSolv Technology Corporation

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1,4-Benzodiazepines in Urine Analyzed using RP and ANP – AppNote

The analyses of 1,4-Benzodiazepines using the Cogent Diol Column from Urine Samples after Solid Phase Extraction using two types of Retention Modes are shown in this Note. Four Compounds were well retained and separated in both HPLC Modes shown in Figures A and B; however, the Retention Order was changed depending on the Gradient used. It […]

1,4-Benzodiazepines in Urine by ANP – AppNote

1,4-Benzodiazepines from Urine Samples were analyzed using a Cogent Diamond Hydride 2.o HPLC Column successfully after Solid Phase Extraction (SPE). Four available Compounds (shown below) were well retained and separated. The procedure could be used for determination of this class of compounds in urine samples and other body fluids. Peaks: 1. Temazepam 301.0739 m/z [M+H]+ […]

3-Hydroxy-3-Methylglutaric acid, HMG in Urine by HPLC – AppNote

Important Biomarker for Diabetes and HMG Disorder is well retained by HPLC. A selective, specific, and sensitive HPLC method with LCMS Analysis has been developed for the determination of 3-hydroxy-3-methylglutaric acid (a.k.a. Meglutol) in urine samples. The method can be also used in the analysis of plasma samples after precipitation of plasma proteins with Acetonitrile. […]

Acetone as the Organic Mobile Phase Component – AppNote

“Green” substitute for separation of amino acids Caution: Acetone and Amines can react When working with Acetone as an HPLC solvent it is important to note that primary amines could react with Acetone to form imines. However, to achieve this reaction the mobile phase would most likely need to be free of water and or […]

Acetyl-L-Carnitine (ALC) in Plasma – AppNote

Excellent LC-MS method in spiked plasma sample For more detailed application, click here. Attachments No 240 Acetyl-L-Carnitine in Plasma.pdf 0.3 Mb Download File

Acrylamide HPLC analysis method – AppNote

Easy & Precise Retention Attachments No 199 Acrylamide.pdf 0.3 Mb Download File

Advantage of Using Cogent TYPE-C Columns for Drugs of Abuse Analysis – AppNote

Analysis of drugs of abuse encompasses a wide variety of compounds, including controlled substances, prescription drugs, and even over-the-counter formulations. These drugs can be problematic to analyze due to several problems. First, many have similar structures (e.g. morphine and hydromorphone), making adequate selectivity difficult to obtain in a chromatographic separation. Second, a good number of […]

Advantages of Cogent HPLC Columns for Analyzing Amazonian Bark Extract – AppNote

The Amazon rainforest is the most biodiverse region in the world. Over one quarter of natural medicines have been found in rainforests, and new species are constantly being discovered. Therefore, there is a need for analytical study of the indigenous flora and fauna in developing new pharmaceuticals. In some cases, natives of the region have […]

Alprazolam (Xanax®) – AppNote

Robust Separation of API from USP Internal Standard Attachments No 183 Alprazolam Xanax.pdf 0.6 Mb Download File

Alprazolam (Xanax®) HPLC Method Transferred – AppNote

Benefit of 2.2um Column Analyzing Alprazolam by HPLC In this AppNote, we show that a transfer from a 4µm to 2.2µm Column, higher efficiency is observed with the smaller particle size Column. This can be very useful for analyses requiring detection at low concentrations. The Peak shape is very sharp using the 2.2um Column (Figure. […]

Amikacin & Tobramycin – AppNote

Highly Polar Antibiotic Separation These two Antibiotic agents present a considerable challenge to chromatographers. As highly hydrophilic compounds, they are generally poorly retained by conventional Reversed Phase methods that rely on an analytes hydrophobicity. In addition, they exhibit very low UV absorption and therefore more sophisticated detection methods are often required. In this application, an […]

Amiloride and Triamterene in Urine – AppNote

LCMS Method for Spiked Urine Sample This application note describes the separation of two important drugs for treatment of hypertension and congestive heart failure. Both compounds have numerous amine groups, which can contribute to Peak tailing in some instances. However, the Peak shapes observed here are both symmetrical and very efficient. This is accomplished using […]

Amino Acids, Non Derivatized – AppNote

From Synthetic Urine Attachments No 58 Non Derivatized Amino Acids.pdf 0.2 Mb Download File

Amoxicillin – AppNote

Orthogonal retention method to USP assay Attachments No 135 Amoxicillin.pdf 0.2 Mb Download File

Analysis of Litchi Seed Extracts – AppNote

Hypoglycin A and Methylenecyclopropylglycine are two compounds found in the extract of Litchi Seeds that studies have shown to be toxic. Methylenecyclopropylglycine inhibits the synthesis of fatty acids from Glucose, and therefore undernourished children are more at risk since their Glucose reserves are low. This study used LC-MS with a Cogent Diamond Hydride™ Column to […]

Analysis of Nucleotides by ANP – AppNote

ATP & AMP separated in Aqueous Normal Phase (ANP) Attachments No 95 Analysis of Nucleotides by ANP.pdf 0.1 Mb Download File

Analysis of Thiopurines – AppNote

Separation using LCMS Gradient Method The Cogent Diamond Hydride Column with gradient elution was used for analysis of Thiopurines. Figure A shows the Peak of 6-TG, which also can be analyzed using Isocratic conditions. Good retention and symmetrical peak shape were obtained under the analysis conditions. Figure B represents two separated Thiopurines (Mono and Di- […]

Analyte Ionization using L-tyrosine – AppNote

L-Tyrosine capsule Attachments No 179 LC-MS Tips for Promoting Analyte Ionization.pdf 0.4 Mb Download File

Anatoxin-A Method Development Strategy for a Polar Compound – AppNote

Using Anatoxin-a Attachments No 142 Method Development Strategy for a Polar Compound.pdf 0.3 Mb Download File

Anatoxin-a, ANTX-A – AppNote

Potent Neurotoxin Attachments No 141 Anatoxin-a ANTX-A.pdf 0.3 Mb Download File

Arginine, Lysine & Histidine, Basic Amino Acids – AppNote

In Synthetic or Human Urine Attachments No 60 Basic Amino Acids.pdf 0.3 Mb Download File

Aspartic Acid & Arginine: Amino Acids – AppNote

Reduced Sample Prep and Handling Attachments No 63 Easy Amino Acid Method.pdf 0.2 Mb Download File

Asymmetric Dimethylarginine ADMA – AppNote

LC-MS method in plasma samples Attachments No 169 Asymmetric Dimethylarginine ADMA.pdf 0.3 Mb Download File

Atenolol Tablet – AppNote

No Ion Pairings Needed Attachments No 250 Atenolol Tablet.pdf 0.4 Mb Download File

ATP, UDP-Glucose UDP-Galactose Separation from Red Blood Cells – AppNote

Separation of Nucleotides In this application, we present an easy-to-use protocol for sensitive detection and quantification of nucleotides in red blood cell extracts. Retention times were very reproducible with %RSD around 0.4. The desired separation between UDP-Glu and UDP-Gal was achieved, all other nucleotides eluted later and did not interfere with the analysis. ATP represents […]

Avoid Sample Cleanup for Food and Beverages – AppNote

3 Methods to Avoid Sample Cleanup with Histamine, Limonin & Folic Acid Food and beverage analysis is complicated by the complex nature of the sample. In many cases, extensive sample cleanup techniques such as Solid Phase Extraction (SPE) may be required to avoid problems associated with the sample matrix. In this Application Note, three strategies […]

B Vitamins Analysis by HPLC – AppNote

Separation of Five Highly Polar Vitamins The B Vitamins are all highly polar and often ion pair agents are needed in Reverse Phase in order to retain them. However, these agents are incompatible with LCMS and therefore limit the applications of these methods. In this method using the Diamond Hydride Column, good separation is obtained […]

B-Vitamin tablet analysis – AppNote

Separation of hydrophilic vitamins in a formulation Attachments No 171 B-Vitamin Tablet Analysis.pdf 0.6 Mb Download File

Baclofen Analysis by HPLC – AppNote

Very Polar API Retained with Precision This compound has two ionizable functional groups (an amine and a carboxylic acid), which makes it quite suitable for retention using this method. A simple gradient produced a very sharp peak for this analyte. These method conditions can be applied to the routine analysis of Baclofen tablet formulations. Peak: […]

Benadryl® Gel Capsule HPLC method – AppNote

Assay method for diphenhydramine HCl without ion pair agents Attachments No 194 Benadryl Gel Capsule.pdf 0.4 Mb Download File

Benzylpiperazine (BZP) – AppNote

Analysis in Hair Samples using LCMS An analytical protocol was developed for analysis of Benzylpiperazine in human hair samples. The elucidation of the chromatographic method was challenging due to the polar nature of BZP. The limits of detection / quantification for this method were determined to be 0.05 ng / mg for Benzylpiperazine in hair […]

Bile Acids from Urine – AppNote

Separation in LCMS using the Diamond Hydride Attachments No 55 Bile Acids from Urine.pdf 0.2 Mb Download File

Bradykinin (BK) Analysis – AppNote

Peptide analyzed in saliva sample Attachments No 236 Bradykinin.pdf 0.3 Mb Download File

Brazilian Tree Bark Analysis by LCMS – AppNote

Identification of Quercetin, Rutin, and “Compound 1” in a Brazilian Tree Bark Over one quarter of natural medicines have been discovered in rainforests, and therefore analytical study of the indigenous flora and fauna is necessary. The Amazonian rainforest in Brazil is the most biodiverse region in the world. A Brazilian tree bark extract (Brownea Grandiceps) […]