Retention with Good Peak Shape for this Polar Compound

This Method illustrates how the MS compatible HPLC UV Methods described in various application notes can be successfully adapted for LCMS.

The Peak Shape for this polar compound meets the System Suitability specification for the USP Tailing Factor.

Ciprofloxacin 332.1405 m/z (M+H)+

Method Conditions
Column: Cogent Diamond Hydride™, 4µm, 100Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150mm
Mobile Phase:
A: 50% DI Water / 50% 2-Propanol / 0.1% Formic Acid (v/v)
B: Acetonitrile / 0.1% Formic Acid (v/v)

Time (minutes) %B
0 90
3 10
6 10
7 90

Post Time: 3 minutes
Injection vol.: 1µL
Flow rate: 0.4mL / minute
Detection: UV @ 254nm
Sample Preparation: A 250mg strength Ciprofloxacin tablet was ground and dissolved in 25mL of 50:50 Solvent A / Solvent B diluent. Solution was sonicated 10 minutes and filtered through 0.45µm Nylon Syringe Filter (MicroSolv Tech Corp.). Sample for injection was diluted 1:100 with the same diluent.
t0: 0.9 minutes

Note: Ciprofloxacin is a Fluoroquinolone antibiotic which acts by inhibition of DNA Gyrase and Topoisomerase IV. It was once used as a drug of last resort for bacterial infections, but accumulated resistance has made the drug less effective in recent years.


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