Additional Specifications for HPLC Low Pressure, PFA Tubing. Specifications

Some extruders of PFA tubing list the specifications below on their website. Physical Density (g/cc): 2.12 – 2.17 Water Absorption (%) < 0.03 Standard Percent Crystallinity (%): 48 – 70 Refraction Index: 1.350 Radiation Resistance (MRad): 1 – 10 Oxygen Index (%): > 95 Mechanical Hardness, Shore D: 55 – 60 Ultimate Tensile Strength (MPa): […]

Read More

Can I use the Flanging Tool for PEEK tubing? FAQ

The “Flanging Tool” (45099-001) or Kit (45099-002) is designed for use with relatively soft tubing that is used with low pressure. It can be used for FEP, ETFE, PFA, or PTFE tubing. Since the flange is made by mechanical means and not by heat the flange is more long lasting and durable, however, the tool is […]

Read More

Can the Super-T PEEK Tubing Items be Used in Medical Applications? FAQ

In general, PEEK is FDA-approved as a long term implantent and can be sterilized. This refers only to the natural type of PEEK tubing and does not include tubing with stripes or markings. The dashed stripes from the Super-T tube can be removed with acetone if desired. However, these tubing items are designed for instrument use and using […]

Read More

How is Pressure Inside Tubing Calculated for HPLC? FAQ

We have a useful Pressure Calculator on our website in which you determine the contribution your tubing ID will bring to the overall back pressure in your system. This can be handy when you are setting up an HPLC system and want to know what ID tubing would be best to use. The lower the […]

Read More

How to Calculate the Internal Volume of Tubing.

To calculate the internal volume (V), you need to know the length of tubing (L) and the inner diameter (ID). You then use the formula for volume of a cylinder. Convert L and ID into cm first. (1 inch = 2.54 cm). This gives V in cm3. 1 cm3 = 1 mL. You can then […]

Read More

How to Cut PEEK Tubing, Use a Clean Cut90 Cutter.

It is Extremely Important to have a Proper Cut for all HPLC Tubing Including PEEK Tubing. The Cutting Blade of the Clean-Cut90™ PEEK Tubing Cutter (shown below) has 2 sides. One side will be marked on the tool with an Arrow, and this produces the Perpendicular Cut (90° angle) that you want to use. Be […]

Read More

How To Cut Stainless Steel Tubing for HPLC.

Cutting Stainless Steel Tubing for HPLC can be challenging. Here are some tips and instructions on cutting tubing using the MicroSolv tubing cutter, Catalog number 46001-01. Introduce the Tubing into the Cutter and lightly Tighten the Screw. If you tighten too much you will deform the Tubing walls. Turn the Cutter a few times around […]

Read More

Maximum Pressure Chart for Stainless Steel Tubing

Below is a chart for the suggested maximum pressure that our stainless steel tubing can withstand under use in HPLC. MTC part no. O.D. (in.) I.D. (in.) I.D. Tolerance (in.) Length (cm) Max. Pressure (psi) 49405-05 1/16″ 0.005 ±0.001 5 21,600 49405-10 1/16″ 0.005 ±0.001 10 21,600 49405-20 1/16″ 0.005 ±0.001 20 21,600 49405-30 1/16″ […]

Read More

Sample Loops

Sample Loops are available in a variety of internal volumes. Some of these will have several turns of the loop but others will not have a loop at all. In the latter case, the reason is because the tubing length is too small to make a loop. Whether or not there is a loop will […]

Read More

Solvents and Solutions that Should be Avoided when Using PEEK Fittings or Tubing.

Solvents and Chemicals that should be Avoided when using PEEK Tubing and Fittings include: Nitric acid Sulfuric acid Halogenated Acids, including hydrofluoric, hydrobromic, and hydroiodic acids* Pure Halogenated gases Methylene chloride THF  (Tetrahydrofuran) DMSO  (Dimethyl Sulfoxide) *Note that you can use Hydrochloric Acid, all other HPLC grade solvents and HPLC buffers with most applications of […]

Read More

What Does 316 in 316 Stainless Steel Mean? FAQ

“316” or “Grade 316” is a class of Stainless Steel (SS) used by the Steel Industry to define a specific Quality; it is a Grade of Steel which MicroSolv offers in our Laboratory Tubing, Fittings and HPLC Columns. According to Atlas Steel Australia, “Grade 316 is the Standard Molybdenum-Bearing Grade, second in importance to 304 […]

Read More

What is the pH range of PEEK laboratory tubing and fittings?

According the some manufacturers of PEEK, the useful pH range of PEEK tubing and fittings for HPLC is 2 – 14 for use with HPLC solvents and buffers. However, I would be very careful with pH 0 to 6 for long term exposure. PEEK is manufactured using Phosphoric Acid and some of the granulate may even still contain […]

Read More

What is the Useful Temperature Range of PEEK Laboratory Products?

The Useful Temperature Range of PEEK related products for Chromatography is up to 100 °C (tubing); up to 125 °C (fittings). PEEK is sometimes rated to 250°C but… It will not be as stable (lower burst pressure for tubing) as it is normally and some Solvents may interact Stronger than under RT with PEEK. PEEK […]

Read More