Tubing Information – MicroSolv Technology Corporation

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Additional Specifications for HPLC Low Pressure, PFA Tubing.

Some extruders of PFA tubing list the specifications below on their website. Physical Density (g/cc): 2.12 – 2.17 Water Absorption (%) < 0.03 Standard Percent Crystallinity (%): 48 – 70 Refraction Index: 1.350 Radiation Resistance (MRad): 1 – 10 Oxygen Index (%): > 95 Mechanical Hardness, Shore D: 55 – 60 Ultimate Tensile Strength (MPa): […]

Are the ends of the Pre-cut Stainless Steel HPLC Tubing polished?

The ends of the pre-cut SS tubing are polished. They have flat, burr free ends and a clean finish. Click HERE for High Pressure HPLC Tubing Ordering Information including SS tubing.

Calculating the internal volume of tubing

To calculate the internal volume (V), you need to know the length of tubing (L) and the inner diameter (ID). You then use the formula for volume of a cylinder. Convert L and ID into cm first. (1 inch = 2.54 cm). This gives V in cm3. 1 cm3 = 1 mL. You can then […]

Can I Use a PEEK Finger Tight Fitting on Stainless Steel Tubing? Can I Use a SS Fitting on PEEK Tubing in HPLC? FAQ

Yes, you can very effectively use PEEK Fittings on Stainless Steel Tubing as long as you take into account the making Pressure Ratings of the Fitting you are using and the expected Pressures in your System. Generally speaking, a PEEK Fitting is preferred for all HPLC Applications up to 5,000 psi and No, you should […]

Can I use the Flanging Tool for PEEK tubing?

The “Flanging Tool” (MicroSolv cat# 45099-001) or Kit (cat# 45099-002) is designed for use with relatively soft tubing that is used with low pressure. It can be used for FEP, ETFE, PFA, or PTFE tubing. Since the flange is made by mechanical means and not by heat the flange is more long lasting and durable, however, the tool […]

Can the MicroSolv Super-T PEEK tubing items be used in medical applications?

In general, PEEK is FDA-approved as a long term implantent and can be sterilized. This refers only to the natural type of PEEK tubing and does not include tubing with stripes or markings. The dashed stripes from the Super-T tube can be removed with acetone if desired. However, these tubing items are designed for instrument use and using […]

Do you offer a low-adsorption tubing suitable for proteins?

The closest tubing we recommend would be FEP or PTFE. PTFE has micro-pores, and proteins may ‘creep’ in there. FEP is preferred because it has the same chemical stability but without the micro-pores. However, you would have to test it for your proteins as we could not guarantee it would be appropriate for every protein […]

How is pressure inside tubing calculated for HPLC?

We have a useful Pressure Calculator on our website in which you determine the contribution your tubing ID will bring to the overall back pressure in your system. This can be handy when you are setting up an HPLC system and want to know what ID tubing would be best to use. The lower the […]

How to Cut PEEK Tubing, Use a Clean Cut90 Cutter.

It is Extremely Important to have a Proper Cut for all HPLC Tubing Including PEEK Tubing. The Cutting Blade of the Clean-Cut90™ PEEK Tubing Cutter (Shown Below) has 2 sides. One side will be marked on the tool with an Arrow, and this produces the Perpendicular Cut (90° angle) that you want to use. Be […]

How To Cut Stainless Steel Tubing for HPLC.

Cutting Stainless Steel Tubing for HPLC can be challenging. Here are some tips and instructions on cutting tubing using the MicroSolv tubing cutter, Catalog number 46001-01. Introduce the Tubing into the Cutter and lightly Tighten the Screw. If you tighten too much you will deform the Tubing walls. Turn the Cutter a few times around […]

Is PEEK Tubing or PEEK Fittings Autoclavable? FAQ

Yes, many Scientists have used PEEK Successfully when Autoclaving PEEK Tubing and or PEEK Fittings using Gamma Irradiation, Heat or Ethylene Oxide sterilization. Click HERE for PEEK Tubing Information

Is Stainless Steel Tubing or Fittings Autoclavable? FAQ

Yes, many Scientists have used 316 Stainless Steel Successfully when Autoclaving Tubing and or Fittings for HPLC using Gamma Irradiation, Heat or Ethylene Oxide Sterilization Methods. Click HERE for Stainless Steel Tubing Information

Maximum Pressure Chart for Stainless Steel Tubing

Below is a chart for the suggested maximum pressure that our stainless steel tubing can withstand under use in HPLC. MTC part no. O.D. (in.) I.D. (in.) I.D. Tolerance (in.) Length (cm) Max. Pressure (psi) 49405-05 1/16″ 0.005 ±0.001 5 21,600 49405-10 1/16″ 0.005 ±0.001 10 21,600 49405-20 1/16″ 0.005 ±0.001 20 21,600 49405-30 1/16″ […]

Sample Loops

Sample Loops are available in a variety of internal volumes. Some of these will have several turns of the loop but others will not have a loop at all. In the latter case, the reason is because the tubing length is too small to make a loop. Whether or not there is a loop will […]

Solvents and Solutions that Should be Avoided when Using PEEK Fittings or Tubing.

Solvents and Chemicals that should be Avoided when using PEEK Tubing and Fittings include: Nitric acid Sulfuric acid Halogenated Acids, including hydrofluoric, hydrobromic, and hydroiodic acids* Pure Halogenated gases Methylene chloride THF (Tetrahydrofuran) DMSO (Dimethyl Sulfoxide) *Note that you can use Hydrochloric Acid, all other HPLC grade solvents and HPLC buffers with most applications of […]

Specifications of different stainless steel grades

Cogent™ HPLC column hardware is stainless steel T1 grade, AISI 316 LC type. HPLC system tubing is AISI 316, T2 grade. Elemental assays of the various stainless steel grades are given in the following table: Stainless Steel Tubing Product Page

What are The Temperature and Pressure Specifications for Stainless Steel Tubing? FAQ

It is very hard to claim any particular Pressure or Temperature rating for Stainless Steel Tubing used in HPLC Applications without knowing the Specifics of Use as there are many Types of SS Tubing used in the Laboratories. Seamless Welded, Double Welded and Annealed Tubing have different Specs making this a difficult Question to answer. […]

What Does 316 in 316 Stainless Steel Mean? FAQ

“316” or “Grade 316” is a class of Stainless Steel (SS) used by the Steel Industry to define a specific Quality; it is a Grade of Steel which MicroSolv offers in our Laboratory Tubing, Fittings and HPLC Columns. According to Atlas Steel Australia, “Grade 316 is the Standard Molybdenum-Bearing Grade, second in importance to 304 […]

What Does Stainless in Stainless Steel Mean? FAQ

According to Wikipedia, Stainless Steel does not Corrode, Rust or Stain like other Carbon Steel does due to the Chromium added to the Steel. Wikidpedia article about Stainless Steel

What is the maximum pressure tolerance for 0.020” ID SS Tubing?

The pressure tolerance for this tubing is 15,800 psi. Please refer to our maximum pressure tolerance chart for full listings of our tubing products. High Pressure Tubing Ordering Information

What is the pH Range of 316 Stainless Steel Laboratory Tubing, Fittings and Sparging Stones? FAQ

The useful pH Range of 316 Stainless Steel Tubing, Fittings and Sparging Stones is 1 – 14. More information about stainless steel tubing

What is the pH range of PEEK laboratory tubing and fittings?

According the some manufacturers of PEEK, the useful pH range of PEEK tubing and fittings for HPLC is 2 – 14 for use with HPLC solvents and buffers. However, I would be very careful with pH 0 to 6 for long term exposure. PEEK is manufactured using Phosphoric Acid and some of the granulate may even still contain […]

What is the Useful Temperature Range of 316 Stainless Steel Laboratory Products?

The useful Temperature Range of Stainless Steel (SS) related products such as Tubing and HPLC Fittings is up to 240°C.

What is the Useful Temperature Range of PEEK Laboratory Products?

The Useful Temperature Range of PEEK related products for Chromatography is up to 100 °C (tubing); up to 125 °C (fittings). PEEK is sometimes rated to 250°C but… It will not be as stable (lower burst pressure for tubing) as it is normally and some Solvents may interact Stronger than under RT with PEEK. PEEK […]

Why is it not recommended to use FEP tubing above 80°C when the melting point is much higher?

FEP has a melting point of 260°C and a general recommended maximum operating temperature during usage of around 200°C. If pressure is applied in the tubing, however, the maximum temperature should be lowered accordingly. The maximum temperature during usage should be 80°C under these conditions. If no pressure is applied, the tubing can be used […]

Category: Tubing Information