How to reduce peak broadening due to poor column connections

The spring-loaded “Direct Adaptive” Di-Ad™ HPLC column connectors were designed for speed and convenience when changing between different column brands on your instrument. 

In the example below, we demonstrate how this error-free process using DiAd fittings can also reduce peak broadening from common poor connections.

Trace Fitting Used Height (mAU) Plates (N)
Black PEEK 31.33 6306
Red Poorly Inserted PEEK 30.36 6023
Blue Di-Ad™ 35.37      7160


Method Conditions:
Direct Adaptive HPLC column connector: Double end fitting

Catalog No.: 49910-10-DD
Dimensions: 0.010″ ID x 1/16th” OD, 100mm long

Column: Cogent Bidentate C8™, 4μm, 100Å.
Catalog No.: 40008-10P
Dimensions: 4.6mm x 100mm
Mobile Phase: 40% acetonitrile / 60% DI water 0.1 % formic acid
Injection vol.: 1µL
Flow rate: 1.0mL / minute
Detection: UV 254nm
Sample Preparation: 0.1mg / mL phenol in mobile phase.

Notes: Column efficiency: theoretical plates (N) 1/2 Height   N = 5.54((tR/W)2)
tR = Retention time of peak
W = Width of peak measured at 1/2 height