10 Phenolic Acids Separated with LCMS – AppNote

Compounds Shown were Extracted from Commercial Rice. Commercial Rice Extract 3 was spiked with ten standards and analyzed. The Cogent Phenyl Hydride Column was an excellent choice to use for analysis of phenolic compounds in the sample with Reversed Phase HPLC. The retention and separation of all nine available standards was possible using the method […]

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5 Phenolic Compounds Separated by LCMS – AppNote

Commercial Rice Extract A commercial rice extract was analyzed and peaks were assigned based on retention times and m/z values for the compounds of interest using the Cogent Phenyl Hydride Column . After method validation the developed procedure can be used to evaluate the quality of rice and to develop the best extraction procedure. Peaks: […]

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Aegeline in Protein Sport Mix – AppNote

LC-MS analysis of harmful substance in food supplement The data demonstrates the use of the Cogent Bidentate C18 2.o™ Column for analysis of dietary supplement samples. Isolation of the Aegeline peak is readily obtained with an extracted ion chromatogram (EIC) in the positive ion mode. Chromatographically, the peak is well retained and highly symmetrical. The […]

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Analysis of Litchi Seed Extracts – AppNote

Hypoglycin A and Methylenecyclopropylglycine are two compounds found in the extract of Litchi Seeds that studies have shown to be toxic. Methylenecyclopropylglycine inhibits the synthesis of fatty acids from Glucose, and therefore undernourished children are more at risk since their Glucose reserves are low. This study used LC-MS with a Cogent Diamond Hydride™ Column to […]

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B Vitamins Analysis by HPLC – AppNote

Separation of Five Highly Polar Vitamins The B Vitamins are all highly polar and often ion pair agents are needed in Reverse Phase in order to retain them. However, these agents are incompatible with LCMS and therefore limit the applications of these methods. In this method using the Diamond Hydride Column, good separation is obtained […]

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Bisphenol A, BPA an HPLC Method – AppNote

Toxic Substance Found in Consumer Products Analyzed by HPLC Bisphenol A (BPA) is a challenging compound for analysis by HPLC. Monitoring of this environmental and health toxicant is a necessary process for surveillance as well as risk assessment. As shown in the chromatograms below (5 overlaid), a Cogent Bidentate C8 Column produces Peak shapes that […]

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Bisphenol A, S, & F Separated by HPLC – AppNote

Separation of Epoxy Resins Found in Consumer Products & Food Many consumer products are using BPA alternatives in recent years due to health concerns regarding toxicity of these compounds.  An analytical HPLC method is shown in this AppNote to distinguish amongst these various Bisphenol compounds potentially in chemicals and foods. Excellent resolution was obtained for […]

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BPA, BPF, and BPS in Water Transferred from Baby Bottles – AppNote

Ever since Bisphenol A (BPA) has become banned in baby bottle products in the U.S. due to its reported endocrine-disrupting effects, manufacturers have had to use alternative materials. Two such materials with properties similar to BPA are Bisphenol S (BPS) and Bisphenol F (BPF). The problem is that these compounds may in fact be just […]

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Brazilian Tree Bark Analysis by LCMS – AppNote

Identification of Quercetin, Rutin, and “Compound 1” in a Brazilian Tree Bark Over one quarter of natural medicines have been discovered in rainforests, and therefore analytical study of the indigenous flora and fauna is necessary. The Amazonian rainforest in Brazil is the most biodiverse region in the world. A Brazilian tree bark extract (Brownea Grandiceps) […]

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Caffeine Content in Coffee Analyzed by HPLC – AppNote

Espresso, Regular, and De-Caffeinated Coffee Caffeine Content Caffeine is separated from other matrix components in three common Coffee beverages in this AppNote. A shot of Espresso (Figure A) had the highest content, followed by regular drip Coffee (Figure B) as expected. In De-Caffeinated Coffee, Caffeine is extracted from the coffee beans but even after numerous […]

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Cyanidin-3-O-Glucoside Analysis in Fruit- AppNote

Analysis in Fruit and Vegetable Extracts In this analysis of proprietary fruit & vegetable extracts, the presence of Cyanidin-3-O-Glucoside was confirmed in three out of four extracts. The peaks were symmetrical and retained beyond the dead volume. The method after validation can be used to quality control of commercial fruit extracts as well in studies […]

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Determination of Phenolic Compound by LCMS – AppNote

Compounds in Olive Leaves Extract A commercial olive extract was analyzed using the Cogent Phenyl Hydride Column. Only one oleuropein peak was detected and it was symmetrical and well retained. The results were reproducible (%RSD = 0.2 for retention times). According to the literature [1] the extract from olive leaves should contain additional compounds. To […]

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Download an Analysis of Harmful Substances in Dietary Supplements – AppNote

Dietary Supplements have had, in notable cases, ingredients added to them that have been implicated in serious health issues and even death. Two such pernicious compounds are Aegeline and 1,3-Dimethylamylamine. These two can be readily separated from the dietary supplement matrix using the Cogent Bidentate C18 2.o™   and Cogent Diamond Hydride™ Columns by LCMS […]

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Fructose and Glucose in Coca-Cola Analyzed by HPLC with RI – AppNote

Separation of Polar Sugars in Coca Cola© The high sugar content of soda beverages can be observed in this Chromatographic separation of Fructose and Glucose in cola. The two Sugars are retained and separated with the use of the Cogent Amide Column. Data from reference standards is also shown for peak identity confirmation. A preconditioning […]

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Galactose and Sucrose – AppNote

Retention and Separation of Mono and Disaccharides This application demonstrates the ability of the Cogent Amide Column to retain two highly polar test solutes. Sucrose, for example, has a log P of -3.7 and hence would be unlikely to retain in Reversed Phase. Excellent separation is obtained here for these Mono and Disaccharides. The noisy […]

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Glycosidic Constituents of Mesquite Flour Analysis with LCMS – AppNote

Observed in Mesquite flour extracts, these compounds are Glycosidic Flavones and Anthocyanins. The Glycoside moieties impart polar characteristics to the analytes, which makes them amenable to retention by the Aqueous Normal Phase (ANP) retention mode. In this application note, the Cogent Phenyl Hydride Column was used to obtain Chromatographic Separation. The same column can be […]

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Glycosidic Flavonoids Analyzed by HPLC – AppNote

Separation of Diosmin, Eriocitrin, and Naringin In this method, three Glycosidic flavonoid standards are separated with good resolution. The Cogent Bidentate C18 2.o™ Column produces high efficiency peaks with reproducible retention (see figure overlay). Separation is also observed for a small impurity peak, eluting between peaks 1 and 2. This separation of standards could be […]

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Histamine Analysis in Tuna by LCMS – AppNote

LC-MS method without derivatization A small amount of Histamine was found in a Tuna sample (Figure A) after an extraction procedure and analysis using the Cogent Diamond Hydride Column and MS detection. In Figure B, a Tuna sample was spiked before the extraction procedure at a level of 0.5 mg/L and Figure C shows the […]

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Histamine Content in Red Wine by LCMS – AppNote

Demonstrating Low Histamine Content in Red Wine Samples Two red wine samples were tested for Histamine using the Cogent Diamond Hydride Column in an LC-MS method. The wine shown in Figure B was claimed to have virtually no Histamine but had no analytical evidence to support it. The claim was verified using this simple LCMS […]

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Histamine Content in Red Wine Using IPA – AppNote

Use Isopropyl Alcohol for Improved Peak Shape of Histamine  This method for red wine analysis is simple, fast, robust and does not require derivatization like many other HPLC methods.  When Isopropanol (IPA) was not used in the A Solvent, the Histamine Peak was highly symmetrical in the standard (Compare Fig. C  and D) but distorted […]

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Histamine in Cheese Analyzed by LCMS – AppNote

No Derivatization Required for this Analysis of Histamine A small but measurable amount of Histamine was found in a Cheese sample (Figure A) after an extraction procedure and analysis using the Cogent Diamond Hydride Column with MS detection. Two spiked Cheese samples were also analyzed. In Figure B, the Cheese sample was spiked before the […]

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L-Theanine in Green Tea – AppNote

L-Theanine in Green Tea L-Theanine does not have significant UV absorption, but detection at a low wavelength (200 nm) was found to be suitable. With an expected sample concentration of around 10 ppm, a calibration curve in the range 2–200 ppm would be appropriate. The data showed good linearity in the studied range, with a […]

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Limonin in Orange Juice Analyzed by HPLC – AppNote

Reproducible Separation from Sample Matrix Peaks An Orange Juice Sample was spiked at the level of 250 ppm of Limonin and filtered (MicroSolv Tech Corp. 0.45μm Nylon Syringe Filter)  before injection. Several Peaks from to the Juice Sample Matrix were separated (peaks1–5), the Limonin peak was well separated from the other Peaks and was easy […]

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Milk Extract Analyzed by LCMS – AppNote

Analysis of Composition using LCMS Milk components are polar compounds that are not well retained or resolved by traditional Reversed Phase Chromatography. In addition, many of the compounds do not contain strong chromophores, resulting in low specificity and sensitivity in UV detection. The analysis using the Cogent Diamond Hydride Column and MS detection provides separation […]

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Mycotoxins – AppNote

The detection of mycotoxins has been an area of increasing focus in ensuring human health due to their high toxicity even at low concentrations. These chemicals are produced by various species of mold, including Aspergillus Penicillium, Paecilomyces, and Fusarium types. Exposure can come from ingestion of infested food products or, in the case of fungal […]

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Oleic Acid & Elaidic Acid – AppNote

Advantages of shape selectivity in cis/trans separations The separation of cis and trans isomers by Reversed Phase can be challenging, as the two compounds often have little difference in hydrophobicity. In cases such as these, additional selectivity mechanisms may be necessary to obtain resolution. Furthermore, they cannot be resolved by MS because they have the […]

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Oleuropein in Olive Leaves Extract Analyzed by HPLC – AppNote

Separation from Matrix Peaks The main peak (Oleuropein) was observed to be well separated from matrix components in this HPLC-UV based approach. This method is useful for analysts that are not using LC-MS and therefore need good Chromatographic Separation in order to perform accurate quantitation. The matrix peaks do not co-elute with the main peak, […]

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Oleuropein in Olive Leaves Extract Analyzed by LCMS – AppNote

High Efficiency Using a near-UHPLC Column for Oleuropein In this AppNote, the Analyte Peak is symmetrical and well retained while the results were very reproducible (%RSD = 0.06 for Retention Times). This Method can be used to analyze and evaluate the extraction of Olive Leaves. According to the literature, Olive Leave Extracts should contain the […]

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Peonidin-3-Glucoside Analyzed by LCMS – AppNote

LC-MS method for Anthocyanin- Fruit or Vegetable Extracts The Cogent Phenyl Hydride™ Column was used for analysis of proprietary fruit or vegetable extracts. The presence of Peonidin-3-O–Glucoside was confirmed in two out of four extracts. The peaks were symmetrical and retained beyond the dead volume. Due to the MS detection and comparison of the retention […]

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